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公开(公告)号：KR101101986B1
公开(公告)日：2012-01-02
申请号：KR1020090038047
申请日：2009-04-30
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 야생형 DsRed(Red Fluorescence Protein) 유전자의 N-말단에 3개의 아미노산인 MGS 및 C-말단에 5개의 아미노산인 CGSPG이 연장되어 이루어지는 적색 형광 단백질 FmRed(fast maturating red fluorescent protein)에 관한 것으로, 본 발명에 따른 적색 형광 단백질 FmRed는 바이오센서, 이중 리포터(dual reporter) 시스템이나 형광공명에너지전달(fluorescence resonance energy transfer, FRET)과 같은 형광 단백질 연동 혹은 연속반응 시스템의 이용에 크게 기여할 것이다.
리포터, 탐침자, 형광 단백질-
公开(公告)号：KR1020100119104A
公开(公告)日：2010-11-09
申请号：KR1020090038047
申请日：2009-04-30
Applicant: 전남대학교산학협력단
Abstract: PURPOSE: A red fluorescent protein FmRed(fast maturating red fluorescent protein) is provided to use in continuous reaction system such as fluorescence resonance energy transfer(FRET). CONSTITUTION: A red fluorescent protein FmRed is formed by elongation of MGS amino acids at N-terminal and CGSPG amino acids at C-terminal. The FmRed has an amino acid sequence of sequence number 2. A FmRed gene contains nucleotide sequence of sequence number 1. A method for preparing FmRed using elongation mutagenesis PCR comprises: a step of performing PCR of defective gene of wild type DsRed to construct a mutant gene library; a step of introducing the library into each cell to express mutant protein; and a step of selecting mutant protein having quick fluorescence and dark red.
Abstract translation: 目的：提供红色荧光蛋白FmRed（快速成熟红色荧光蛋白）用于连续反应系统，如荧光共振能量转移（FRET）。 构成：红色荧光蛋白FmRed由C末端的N-末端的MGS氨基酸和CGSPG氨基酸延伸形成。 FmRed具有序列号2的氨基酸序列.FmRed基因含有序列号1的核苷酸序列。使用延伸诱变PCR制备FmRed的方法包括：对野生型DsRed的缺陷型基因进行PCR以构建突变体 基因库; 将文库引入每个细胞以表达突变蛋白的步骤; 以及选择具有快速荧光和深红色的突变蛋白的步骤。
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