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公开(公告)号：KR1020110124828A
公开(公告)日：2011-11-18
申请号：KR1020100044235
申请日：2010-05-12
Applicant: 전남대학교산학협력단
IPC: C12N5/0735 , C13K1/00 , C07K14/78 , C12N5/02
Abstract: PURPOSE: A method for enhancing fibronectin using glucose of high concentration is provided to stimulate G1 progress and to proliferate embryonic stem cells. CONSTITUTION: A method for enhancing fibronectin synthesis is performed through angiotensin II and deformability growth factor-beta1(TGF-beta1) in case of treating mouse embryonic stem cells with 25mM or more of glucose. The glucose of high concentration activates angiotensin II type 1 receptor. The mouse embryonic stem cells are ES-E14TG2a or ES-R1. A medium for proliferating mouse embryonic stem cells contains 25mM or more of glucose.
Abstract translation: 目的：提供使用高浓度葡萄糖增强纤连蛋白的方法，以刺激G1进展并增殖胚胎干细胞。 构成：在用25mM或更多葡萄糖处理小鼠胚胎干细胞的情况下，通过血管紧张素II和变形性生长因子-β1（TGF-β1）进行增强纤连蛋白合成的方法。 高浓度葡萄糖激活血管紧张素II 1型受体。 小鼠胚胎干细胞是ES-E14TG2a或ES-R1。 用于增殖小鼠胚胎干细胞的培养基含有25mM或更多的葡萄糖。
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公开(公告)号：KR1020110008672A
公开(公告)日：2011-01-27
申请号：KR1020090066138
申请日：2009-07-21
Applicant: 전남대학교산학협력단
Abstract: PURPOSE: A method for inducing proliferation of mouse embryo stem cells using linoleic acid is provided to enhance activation of cell cycle regulatory proteins in the stem cells. CONSTITUTION: A mouse embryo stem cell is proliferated by linoleic acid. A method for inducing proliferation of the mouse embryo stem cells comprises: a step of activating G protein-coupled receptor 40(GPR40) to stimulate phospholipase C; a step of activating protein kinase C(PKC); a step of activating p44/42 mitogen-activated protein kinase(MAPK); and a step of increasing activation of cell cycle regulatory protein of the stem cells. A medium for proliferating the stem cells contains 10^-7-10^-9 M of linoleic acid.
Abstract translation: 目的：提供使用亚油酸诱导小鼠胚胎干细胞增殖的方法，以增强干细胞中细胞周期调节蛋白的活化。 构成：小鼠胚胎干细胞被亚油酸增殖。 诱导小鼠胚胎干细胞增殖的方法包括：激活G蛋白偶联受体40（GPR40）以刺激磷脂酶C的步骤; 激活蛋白激酶C（PKC）的步骤; 激活p44 / 42丝裂原活化蛋白激酶（MAPK）的步骤; 以及增加干细胞的细胞周期调节蛋白活化的步骤。 用于增殖干细胞的培养基含有10 -7〜10 -9 M的亚油酸。
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公开(公告)号：KR1020110054181A
公开(公告)日：2011-05-25
申请号：KR1020090110740
申请日：2009-11-17
Applicant: 전남대학교산학협력단
Abstract: PURPOSE: A method for inducing the proliferation of embryonic stem cells using caveolin-1 is provided to induct extracellular signal-regulated kinase1/2(Erk1/2) and mouse embryonic stem cell proliferation. CONSTITUTION: A method for inducing the proliferation mouse embryonic stem cells using caveolin-1 comprises: a step of activating caveolin-1 and focal adhesion kinase(FAK); a step of activating phosphatidylinositol 3-kinase(PI3K)/protein kinase(Akt) to induce ERK1/2; a step of increasing cell cycle regulatory protein and MMP-2 expressions. A medium for enhancing the proliferation of the mouse embryonic stem cell contains caveolin-1.
Abstract translation: 目的：提供使用窖蛋白-1诱导胚胎干细胞增殖的方法，以诱导细胞外信号调节激酶1/2（Erk1 / 2）和小鼠胚胎干细胞增殖。 构成：使用窖蛋白-1诱导增殖小鼠胚胎干细胞的方法包括：激活窖1和粘着斑激酶（FAK）的步骤; 激活磷脂酰肌醇3-激酶（PI3K）/蛋白激酶（Akt）以诱导ERK1 / 2的步骤; 增加细胞周期调控蛋白和MMP-2表达的一个步骤。 用于增强小鼠胚胎干细胞增殖的培养基含有窖1。
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Patent Agency Ranking
公开(公告)号：KR100836827B1
公开(公告)日：2008-06-10
申请号：KR1020070034482
申请日：2007-04-09
Applicant: 전남대학교산학협력단
CPC classification number: C12M21/06 , C12M23/10 , C12M25/06 , C12N5/0606
Abstract: A cell culture dish is provided to increase the differentiation efficiency of embryonic stem cells due to being free from physical damage of an embryoid body and contamination by microorganism during a process of moving the embryoid body into a floating culture medium and omit a suspension culturing step by supplying enough nutrition to the embryoid body. A cell culture dish(10) for forming an embryoid body from embryonic stem cells comprises: a petridish(50) where a receiving space with a certain depth is formed; a cell culture dish cover(20) which opens and closes the petridish; and a plurality of supports(30) which are equipped at the lower portion of the cover and have a groove(40) with a certain shape at an end of the lower portion, wherein the culture dish is made of polycarbonate, polyethylene, polypropylene, or glass.
Abstract translation: 提供细胞培养皿以增加胚胎干细胞的分化效率，这是由于在将胚状体移动到浮游培养基中的过程中没有胚状体的物理损伤和微生物的污染，并且省略了悬浮培养步骤 为胚状体提供足够的营养。 用于从胚胎干细胞形成胚状体的细胞培养皿（10）包括：形成具有一定深度的接收空间的眼（50）; 细胞培养皿盖（20），其打开和关闭花瓣; 以及多个支撑件（30），其设置在所述盖的下部并且在所述下部的端部具有一定形状的槽（40），其中所述培养皿由聚碳酸酯，聚乙烯，聚丙烯， 或玻璃。
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公开(公告)号：KR101185893B1
公开(公告)日：2012-09-25
申请号：KR1020090124373
申请日：2009-12-15
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 저산소증 유도 아폽토시스를 억제하는 미드카인의 배아줄기세포 보호방법에 관한 것이다. 더욱 상세하게는 지질단백질 수용체-관련 단백질-1(LRP-1)을 통한 HIF-1α를 활성화시켜 아폽토시스를 억제하고, 카탈라제의 활성화 및 c-Jun N-말단 키나제(JNK)/스트레스-활성화 단백질 키나제(SAPK) 및 핵 전사인자 NF-κB의 하향 조절을 통해 마우스 배아줄기세포의 저산소증 유도 아폽토시스를 억제하는 미드카인의 배아줄기세포 보호방법 및 상기 미드카인을 아폽토시스 억제 유효 물질로 포함하는 배아줄기세포 증식 배지에 관한 것이다.
배아줄기세포, 미드카인, 아폽토시스, 배지, 저산소증-
公开(公告)号：KR101097767B1
公开(公告)日：2011-12-22
申请号：KR1020090066138
申请日：2009-07-21
Applicant: 전남대학교산학협력단
Abstract: 본발명은리놀레산을이용한마우스배아줄기세포의증식을유도시키는방법에관한것이다. 더욱상세하게는리놀레산은 G 단백질-커플링수용체 40(GPR40)을활성화하여포스포리파아제 C를자극한후 단백질키나제 C(PKC)를활성화시켜 p44/42 미토겐-활성화된단백질키나제(MAPK) 활성화하여마우스배아줄기세포의세포주기조절단백질의활성화를증가시킴으로써마우스배아줄기세포의증식을유도하는방법및 리놀레산을포함하는마우스배아줄기세포증식용배지에관한것이다.
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公开(公告)号：KR1020110067680A
公开(公告)日：2011-06-22
申请号：KR1020090124373
申请日：2009-12-15
Applicant: 전남대학교산학협력단
Abstract: PURPOSE: A method for protecting embryonic stem cells using midkine which suppresses hypoxia-induced apoptosis is provided to activate HIF-1alpha. CONSTITUTION: A method for protecting embryonic stem cells comprises a step of treating midkine for HIF-1 alpha activation and hypoxia-induced apoptosis suppression. A medium for proliferating the embryonic stem cells contains midkine as an active ingredient for suppressing apoptosis.
Abstract translation: 目的：提供一种使用抑制缺氧诱导的细胞凋亡的中期因子来保护胚胎干细胞的方法，以激活HIF-1α。 构成：保护胚胎干细胞的方法包括治疗中期因子以进行HIF-1α激活和缺氧诱导的凋亡抑制的步骤。 用于增殖胚胎干细胞的培养基含有midkine作为抑制凋亡的活性成分。
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公开(公告)号：KR1020080024868A
公开(公告)日：2008-03-19
申请号：KR1020060089576
申请日：2006-09-15
Applicant: 전남대학교산학협력단
Abstract: Water channel proteins aquaporins which participate in water movement are provided to promote secretion of vaginal lubricating liquid. A vaginal lubricant is also provided to promote secretion of vaginal lubricating liquid by modulating expressions of the water channel proteins. A vaginal lubricant for promoting secretion of vaginal lubricating liquid modulates the expressions of water channel proteins aquaporins including aquaporin-1, aquaporin-2, aquaporin-3 and aquaporin-8, wherein the aquaporin-1 is expressed in capillary blood vessel, venule and smooth muscle in vaginal tissue, and the aquaporin-2 and aquaporin-3 are expressed in vaginal tissue epithelial cytoplasm.
Abstract translation: 提供参与水运动的水通道蛋白质水通道蛋白，以促进阴道润滑液的分泌。 还提供阴道润滑剂以通过调节水通道蛋白的表达来促进阴道润滑液的分泌。 用于促进阴道润滑液分泌的阴道润滑剂调节水通道蛋白水通道蛋白的表达，包括水通道蛋白1，水通道蛋白2，水通道蛋白3和水通道蛋白-8，其中水通道蛋白-1在毛细血管，小静脉和平滑肌中表达 肌肉在阴道组织中，水通道蛋白2和水通道蛋白-3在阴道组织上皮细胞质中表达。
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