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1.发明公开
公开(公告)号：KR1020140104234A
公开(公告)日：2014-08-28
申请号：KR1020130018159
申请日：2013-02-20
Applicant: 전북대학교산학협력단 , (주)엔비엠
Abstract: The present invention relates to a method for high production of thermostable recombinant arabinanase enzyme originated from phanerochaete chrysosporium. If arabinanase and xylanase produced from transformed yeast of the present invention are processed in biomass, the efficiency of biomass decomposition is increased by synergism of the two enzymes. Arabinanase of the present invention has high value in industrial utilization as a coenzyme added to feed additives, additives for paper manufacture, detergent additives, additives for biomass preprocess to produce bio-energy, etc.
Abstract translation: 本发明涉及一种高产量来源于金孢子虫的热稳定性重组阿拉伯聚糖酶的方法。 如果由本发明的转化酵母产生的阿拉伯聚糖酶和木聚糖酶在生物质中加工，则通过两种酶的协同作用增加生物质分解的效率。 本发明的阿拉伯糖酶在添加到饲料添加剂中的辅酶，造纸用添加剂，洗涤剂添加剂，用于生产生物能源的生物质预处理添加剂等方面具有高的工业利用价值。
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公开(公告)号：KR1020120052794A
公开(公告)日：2012-05-24
申请号：KR1020100114104
申请日：2010-11-16
Applicant: 전북대학교산학협력단 , 재단법인 전주농생명소재연구원 , (주)엔비엠
Abstract: PURPOSE: A recombinant vector for Oriza sativa transformation and a method for preparing bovine trypsin using the same are provided to obtain a large amount of trypsin through a simple process. CONSTITUTION: A recombinant vector for Oriza sativa transformation comprises an Oriza sativa-derived alpha-amylase 3D(RAmy3D) promoter and a gene encoding bovine trypsin which is operatively linked in a transcriptional direction. The promoter and gene has a base sequence of sequence numbers 1 and 2. The recombinant vector is Pmyt111. A transgenic Oriza sativa is prepared by the recombinant vector. A method for preparing a large amount of bovine trypsin comprises: a step of constructing the recombinant vector; a step of transforming the recombinant vector to Oriza sativa callus; and a step of culturing the callus.
Abstract translation: 目的：提供用于转基因植物的重组载体和使用其的牛胰蛋白酶的制备方法，以通过简单的方法获得大量的胰蛋白酶。 构成：用于苜蓿苜蓿转化的重组载体包含渥太华苜蓿来源的α-淀粉酶3D（RAmy3D）启动子和编码牛胰蛋白酶的基因，其在转录方向上可操作地连接。 启动子和基因具有序列号1和2的碱基序列。重组载体是Pmyt111。 通过重组载体制备转基因玉米螟。 制备大量牛胰蛋白酶的方法包括：构建重组载体的步骤; 将重组载体转化到Oriza sativa愈伤组织的步骤; 和培养愈伤组织的步骤。
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公开(公告)号：KR1020030014976A
公开(公告)日：2003-02-20
申请号：KR1020010048982
申请日：2001-08-14
Applicant: 전북대학교산학협력단
IPC: C12N5/14
CPC classification number: C12P21/02 , C07K14/535 , C12N5/0025 , C12N15/8257 , C12N2501/998 , C12N2510/00 , C12N2510/02
Abstract: PURPOSE: A method for mass-producing recombinant proteins using plant cells is provided, thereby cheaply mass-producing human granulocyte macrophage colony stimulating factors(hGM-CSF). CONSTITUTION: The method for mass-producing recombinant proteins using plant cells is characterized by adding at least one polymeric compound selected from the group consisting of gelatin, polyethylene glycol(PEG) and polyvinylpyrrolidine(PVP) into a medium for culturing a transformant, wherein the transformant is capable of expressing human granulocyte macrophage colony stimulating factor(hGM-CSF); the polymeric compound is added in an amount of 0.5 to 5 wt.%; the polymeric compound is added within 6 days of the cultivation of the transformant; and the transformant is Nicotiana tabacum pMYO64(KCTC 0670BP).
Abstract translation: 目的：提供使用植物细胞大规模生产重组蛋白的方法，从而廉价地批量生产人粒细胞巨噬细胞集落刺激因子（hGM-CSF）。 构成：使用植物细胞大量生产重组蛋白质的方法的特征在于将至少一种选自明胶，聚乙二醇（PEG）和聚乙烯吡咯烷酮（PVP）的聚合物）加入培养转化体的培养基中，其中 转化体能够表达人粒细胞巨噬细胞集落刺激因子（hGM-CSF）; 以0.5〜5重量％的量加入高分子化合物; 在培养转化体后6天内加入高分子化合物; 并且转化体是烟草pMYO64（KCTC 0670BP）。
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4.发明授权백색 부후균 유래의 탄수화물 결합 모듈을 갖는 재조합 아세틸 자일란 에스터라아제 다기능 효소의 고생산 방법 有权用于从Phanerochaete chrysosporium的碳水化合物结合模块生产重组双功能乙酰木聚糖酯酶的方法
公开(公告)号：KR101439965B1
公开(公告)日：2014-09-12
申请号：KR1020130013785
申请日：2013-02-07
Applicant: 전북대학교산학협력단 , (주)엔비엠
Abstract: 본 발명은 백색 부후균 유래의 탄수화물 결합 모듈을 갖는 재조합 아세틸 자일란 에스터라아제 다기능 효소의 고생산 방법에 관한 것으로, 백색 부후균 유래의 탄수화물 결합 모듈 (CBM)을 갖는 아세틸 자일란 에스터라아제 유전자로 형질전환된 효모에서 생산된 아세틸 자일란 에스터라아제는 자일란 구조 (backbone)의 아세틸기를 제거하고 자일란 및 리그닌을 분해하는 활성을 가질 뿐만 아니라, 자일라나아제와 동시에 사용했을 때 바이오매스 분해 활성을 증가시킬 수 있으므로 사료 첨가제, 종이 제조, 세제, 리그노셀룰로오스계 바이오매스 분해 및 바이오 에너지 생산 등을 위한 산업적 효소로 유용하게 이용할 수 있을 것으로 기대된다.
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公开(公告)号：KR1020140100715A
公开(公告)日：2014-08-18
申请号：KR1020130013785
申请日：2013-02-07
Applicant: 전북대학교산학협력단 , (주)엔비엠
Abstract: The present invention relates to a method for high production of recombinant bifunctional acetyl xylan esterase with a carbohydrate binding module derived from phanerochaete chrysosporium. Acetyl xylan esterase produced from yeast which is transformed to an acetyl xylan esterase gene with a carbohydrate binding module(CBM) derived from phanerochaete chrysosporium not only has an activity of removing an acetyl group of a xylan backbone structure and decomposing xylan and lignin, but also improves an activity of decomposing biomass when used with xylanase at the same time, thereby being used as an industrial enzyme for feed additives, production of paper, a detergent, decomposition of lingo-cellulose-based biomass, and production of bio-energy.
Abstract translation: 本发明涉及一种高产量生产重组双功能乙酰木聚糖酯酶的方法，该方法具有衍生自phanerochaete chrysosporium的碳水化合物结合模块。 由酵母生产的由乙酰木聚糖酯酶基因转化成乙酰木聚糖酯酶基因的乙酰木聚糖酯酶不仅具有去除木聚糖骨架结构的乙酰基并分解木聚糖和木质素的活性， 提高了与木聚糖酶同时使用时分解生物质的活性，从而被用作饲料添加剂的工业酶，纸，洗涤剂的生产，基于纤维素的生物质的分解和生物能的生产。
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Patent Agency Ranking
公开(公告)号：KR1020130087709A
公开(公告)日：2013-08-07
申请号：KR1020120008771
申请日：2012-01-30
Applicant: 전북대학교산학협력단 , (주)엔비엠
Abstract: PURPOSE: A method for purifying recombinant human chymotrypsinogen B2 from a transgenic rice cell culture liquid is provided to easily purify a large amount of the culture liquid with a high purity of 90% and reproducibility. CONSTITUTION: A method for purifying recombinant human Chymotrypsinogen B2 which is expressed in a transgenic rice cell culture liquid comprises the steps of: adjusting the Ph concentration of the culture liquid using strong acid to pH 2.5-3.5; incubating the culture liquid at 4 °C for 12-24 hours and centrifuging the culture liquid; removing salts and impurities from the culture liquid using an ultrafilter membrane and concentrating; and adsorbing chymotrypsinogen B2 from the concentrated culture liquid using a strong cation exchanger.
Abstract translation: 目的：提供一种从转基因水稻细胞培养液中纯化重组人糜蛋白酶原B2的方法，以高纯度90％和重复性容易地纯化大量的培养液。 构成：用于纯化在转基因水稻细胞培养液中表达的重组人糜蛋白酶原B2的方法包括以下步骤：使用强酸将培养液的Ph浓度调节至pH 2.5-3.5; 将培养液在4℃下孵育12-24小时，离心培养液; 使用超滤膜从培养液中除去盐和杂质并浓缩; 并使用强阳离子交换剂从浓缩培养液中吸附糜蛋白酶原B2。
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公开(公告)号：KR3006948760000S
公开(公告)日：2013-05-30
申请号：KR3020120011377
申请日：2012-03-09
Applicant: 전북대학교산학협력단
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8.发明授权백색 부후균 유래의 재조합 자일로시다아제/아라비노퓨라노시다아제 다기능 효소의 고생산 방법 有权用于高效生产重组双功能木糖苷酶/阿拉伯呋喃糖苷酶的方法来自PHANEROCHAETE CHROSOSPORIUM
公开(公告)号：KR101418784B1
公开(公告)日：2014-07-11
申请号：KR1020130012708
申请日：2013-02-05
Applicant: 전북대학교산학협력단 , (주)엔비엠
Abstract: The present invention relates to a method for highly producing a multi-functional recombinant xylosidase/arabinofuranosidase enzyme which is derived from a phanerochaete chrysosporium. The present invention is able to largely produce a recombinant β-xylosidase/α-L-arabinofuranosidase which is a decomposition enzyme of hemicellulose which can be widely used in feed additives, paper manufacturing, detergent, and bio energy production by using a yeast (Pichia pastoris) transformed from a β-xylosidase/α-L- arabinofuranosidase gene which is derived from a Phanerochate chrysosporium.
Abstract translation: 本发明涉及一种高度生产多功能重组木糖苷酶/阿拉伯呋喃糖苷酶的方法，所述多功能重组木糖苷酶/阿拉伯呋喃糖苷酶衍生自金孢子虫（Phanerochaete chrysosporium）。 本发明能够在很大程度上产生作为半纤维素分解酶的重组β-木糖苷酶/α-L-阿拉伯呋喃糖苷酶，其可以广泛用于饲料添加剂，造纸，洗涤剂和使用酵母的生物能量生产（毕赤氏酵母 巴斯德毕赤氏酵母）从衍生自Phanerochate chrysosporium的β-木糖苷酶/α-L-阿拉伯呋喃糖苷酶基因转化。
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公开(公告)号：KR101244025B1
公开(公告)日：2013-03-14
申请号：KR1020100114104
申请日：2010-11-16
Applicant: 전북대학교산학협력단 , 재단법인 전주농생명소재연구원 , (주)엔비엠
Abstract: 본 발명은 벼 형질전환용 재조합 벡터 및 이를 이용한 소 트립신의 대량생산방법에 관한 것이다.
본 발명의 벼 형질전환용 재조합 벡터로 형질전환된 벼에서 매우 간단한 공정을 통해 트립신을 대량으로 생산할 수 있으며 생산효율 역시 배지 1L 당 15㎎ 이상으로 매우 높다.-
公开(公告)号：KR1020120128375A
公开(公告)日：2012-11-27
申请号：KR1020110046276
申请日：2011-05-17
Applicant: 전북대학교산학협력단
IPC: A01G7/04 , A01G9/00 , F21V21/36 , F21V29/56 , F21V29/70 , F21Y101/02 , F21Y103/00
CPC classification number: A01G7/045 , A01G9/00 , A01G9/20 , F21V21/36 , F21V29/56 , F21V29/70 , F21Y2101/00 , Y10S47/06
Abstract: PURPOSE: An LED lamp cultivating apparatus for a plant factory is provided to adjust the distance among plants and an LED lamp and the light amount from the LED lamp by the growth speed of the plants. CONSTITUTION: An LED lamp cultivating apparatus for a plant factory comprises the following: a frame body(10) including a plant cultivation space; a cultivation box(20) located on the bottom of the frame body for planting plants; an LED lamp(30) installed on the upper side of the cultivation box for lighting plants; a lifting member(60) ascending and descending a supporter for fixing the LED lamp for adjusting the light amount and the distance among plants and the LED lamp; LEDs(32) attached to the LED lamp, and installed on a printed circuit board(34); and a water-cooled heat radiation member for installing the printed circuit board.
Abstract translation: 目的：提供一种用于工厂的LED灯培养装置，以通过植物的生长速度来调节植物和LED灯之间的距离以及来自LED灯的光量。 构成：用于植物厂的LED灯培养装置包括：包括植物栽培空间的框体（10）; 位于框架体底部用于种植植物的栽培箱（20）; 安装在用于照明设备的栽培箱的上侧的LED灯（30） 提升构件（60），用于上升和下降用于固定LED灯的支撑件，用于调节植物和LED灯之间的光量和距离; 连接到LED灯的LED（32），并安装在印刷电路板（34）上; 以及用于安装印刷电路板的水冷散热构件。
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