公开(公告)号：KR1020030014976A

公开(公告)日：2003-02-20

申请号：KR1020010048982

申请日：2001-08-14

Applicant: 전북대학교산학협력단

Inventor： 이재화 ,  권태호 ,  양문식

IPC: C12N5/14

CPC classification number: C12P21/02 ,  C07K14/535 ,  C12N5/0025 ,  C12N15/8257 ,  C12N2501/998 ,  C12N2510/00 ,  C12N2510/02

Abstract: PURPOSE: A method for mass-producing recombinant proteins using plant cells is provided, thereby cheaply mass-producing human granulocyte macrophage colony stimulating factors(hGM-CSF). CONSTITUTION: The method for mass-producing recombinant proteins using plant cells is characterized by adding at least one polymeric compound selected from the group consisting of gelatin, polyethylene glycol(PEG) and polyvinylpyrrolidine(PVP) into a medium for culturing a transformant, wherein the transformant is capable of expressing human granulocyte macrophage colony stimulating factor(hGM-CSF); the polymeric compound is added in an amount of 0.5 to 5 wt.%; the polymeric compound is added within 6 days of the cultivation of the transformant; and the transformant is Nicotiana tabacum pMYO64(KCTC 0670BP).

Abstract translation: 目的：提供使用植物细胞大规模生产重组蛋白的方法，从而廉价地批量生产人粒细胞巨噬细胞集落刺激因子（hGM-CSF）。 构成：使用植物细胞大量生产重组蛋白质的方法的特征在于将至少一种选自明胶，聚乙二醇（PEG）和聚乙烯吡咯烷酮（PVP）的聚合物）加入培养转化体的培养基中，其中 转化体能够表达人粒细胞巨噬细胞集落刺激因子（hGM-CSF）; 以0.5〜5重量％的量加入高分子化合物; 在培养转化体后6天内加入高分子化合物; 并且转化体是烟草pMYO64（KCTC 0670BP）。

公开(公告)号：KR1020120110295A

公开(公告)日：2012-10-10

申请号：KR1020110028060

申请日：2011-03-29

Applicant: 전북대학교산학협력단

Inventor： 이재화 ,  박현진 ,  진은정 ,  박승문

IPC: C12N1/12 ,  C12Q1/02

Abstract: PURPOSE: A medium composition for culturing algae and a culturing method are provided to improve product amount of micro algae and to recue carbon dioxide. CONSTITUTION: A medium composition for culturing microalgae contains 0.4-1.0% of alginic acid as an active ingredient. The microalgae are Nannochloropsis sp. Nannochloropsis sp. is Nannochloropsis oculata. The medium is prepared with a composition containing alginic acid. A method for culturing microalgae comprises a step of culturing the microalgae using the culture medium at pH 7.0-pH 8.75.

Abstract translation: 目的：提供用于培养藻类的培养基组合物和培养方法，以改善微藻的产物量和再生二氧化碳。 构成：用于培养微藻的培养基组合物含有0.4-1.0％的藻酸作为活性成分。 微藻是Nannochloropsis sp。 南芥属 是Nannochloropsis oculata。 该培养基用含有海藻酸的组合物制备。 培养微藻的方法包括使用pH 7.0〜pH 8.75的培养基培养微藻的步骤。