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公开(公告)号：KR101371416B1
公开(公告)日：2014-03-19
申请号：KR1020120035213
申请日：2012-04-05
Applicant: 전북대학교산학협력단
Abstract: 본 발명은 밀기울 (wheat bran), 인산이수소칼륨 (KH
2 PO
4 ) 및 황산수소칼륨 (KHSO
4 )을 포함하는 것을 특징으로 하는 자일라나아제 (xylanase)를 생산하는 재조합 피키아 파스토리스 (
Pichia
pastoris ) 균주의 자일라나아제 (xylanase) 생산용 배지 조성물 및 자일라나아제를 생산하는 재조합 피키아 파스토리스 균주를 상기 자일라나아제 (xylanase) 생산용 배지 조성물이 함유된 발효조에 투입하여 배양하는 단계를 포함하는 자일라나아제 생산 방법을 제공한다.-
2.发明公开
公开(公告)号：KR1020130113006A
公开(公告)日：2013-10-15
申请号：KR1020120035213
申请日：2012-04-05
Applicant: 전북대학교산학협력단
Abstract: PURPOSE: Xylanase produced by recombinant Pichia pastoris is provided to largely reduce the amount of chemicals needed for bleaching craft pulp, thereby being used as an eco-friendly bleaching agent. CONSTITUTION: A medium composition for recombinant Pichia pastoris strains for producing xylanase contains 7.56-15.22 g/L of wheat bran, 0.13-1.46 g/L of potassium dihydrogen phosphate (KH_2PO_4), and 0.02-0.25 g/L of potassium hydrogen sulfate (KHSO_4). A method for producing xylanase comprises the step of: putting the recombinant Pichia pastoris strains in the medium composition; and culturing the recombinant Pichia pastoris strains. The recombinant Pichia pastoris strains are cultured at 24-27°C, a pH concentration of 5.5-6.5, a stirring rate of 160-180 rpm, and an air flow rate of 0.5-1.5 vvm under the presence of 0.5-1.25% methanol.
Abstract translation: 目的：提供重组巴斯德毕赤酵母产生的木聚糖酶，大大减少漂白工艺纸浆所需化学品的用量，从而被用作环保漂白剂。 构成：用于生产木聚糖酶的重组毕赤酵母菌株的培养基组合物含有7.56-15.22g / L的麦麸，0.13-1.46g / L的磷酸二氢钾（KH_2PO_4）和0.02-0.25g / L的硫酸氢钾（ KHSO_4）。 制备木聚糖酶的方法包括以下步骤：将重组毕赤酵母菌株置于培养基组合物中; 并培养重组毕赤酵母菌株。 重组巴斯德毕赤酵母菌株在0.5〜0.25％甲醇存在下，在24-27℃，pH 5.5〜6.5，搅拌速度160〜180rpm，空气流速0.5〜1.5vvm下培养 。
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