Abstract:
A method of increasing isoflavone content by hydropriming and osmopriming treatment of soybeans consecutively is provided to increase the isoflavone content by 10 to 40% due to soybeans. The germinated soybeans with increased isoflavone content are used to manufacture high functional fermented soybeans. Soybeans are soaked in purified water at 20 to 25deg.C for 4 to 6hr, hydrated, soaked in inorganic salt solutions for 4 to 6hr and germinated at 20 to 25deg.C and 85% relative humidity for 24 to 48hr in an incubator to give germinated soybeans with increased isoflavone content. The inorganic salt is any one selected from NaCl, KNO3 or MgSO4. High functional fermented soybeans are obtained by steaming the germinated soybeans with increased isoflavone content at 121deg.C for 60min, cooling to 50deg.C, inoculating with 2% by volume of Bacillus subtilis cultures and fermenting at 40deg.C for 72hr.