公开(公告)号：KR1020130072533A

公开(公告)日：2013-07-02

申请号：KR1020110140000

申请日：2011-12-22

Applicant: 조선대학교산학협력단

Inventor： 유진철 ,  조승식 ,  최윤희 ,  박다정 ,  송재경 ,  성치남 ,  유하영

IPC: C12N9/20 ,  C12N1/20 ,  C07K1/16 ,  C07K16/40

Abstract: PURPOSE: A lipase produced by Streptomyces sp. is provided to catalyze transesterification for producing biodiesel and to properly produce biodiesel. CONSTITUTION: A lipase for producing biodiesel is isolated from Streptomyces sp. (CS326) and is identified to have a single protein band through SDS-PAGE. The molecular weight of the lipase is 17 kDa. The optimal reaction temperature and pH of the lipase are 40 deg. C and pH 7.0. The lipase is a heat resistant enzyme. The lipid decomposition activity is increased to 100% by adding Triton X-100 and is suppressed to 5% by adding SDS. The lipid decomposition activity is completely suppressed in deoxycholic acid and is suppressed by 50% using EDTA and EGTA. The lipid decomposition activity is suppressed by Mg^2+, Fe^2+, Na^+, Mn^2+, Ca^2+, and Co^2+. The rate constant is Km=0.24mM and V_max=4.6mM/min/mg using Lineweaver-Burk plot.

Abstract translation: 目的：由链霉菌属（Streptomyces sp。）生产的脂肪酶 被提供用于催化酯交换以生产生物柴油并适当地生产生物柴油。 构成：用于生产生物柴油的脂肪酶是从链霉菌属（Streptomyces sp。 （CS326），并通过SDS-PAGE鉴定为具有单个蛋白质条带。 脂肪酶的分子量为17 kDa。 脂肪酶的最佳反应温度和pH为40度。 C和pH 7.0。 脂肪酶是耐热酶。 通过加入Triton X-100将脂质分解活性提高至100％，并通过加入SDS将其抑制至5％。 脂肪分解活性在脱氧胆酸中完全抑制，并使用EDTA和EGTA抑制50％。 脂肪分解活性受到Mg ^ 2 +，Fe ^ 2 +，Na +，Mn ^ 2 +，Ca ^ 2 +和Co ^ 2 +的抑制。 使用Lineweaver-Burk图，速率常数为Km = 0.24mM，V_max = 4.6mM / min / mg。