公开(公告)号：KR100442574B1

公开(公告)日：2004-08-02

申请号：KR1020010053264

申请日：2001-08-31

Applicant: 주식회사농심

Inventor： 김재훈 ,  남희섭 ,  최인재 ,  남수완 ,  김재범

IPC: C12N1/16

Abstract: PURPOSE: Provided are a novel Saccharomyces cerevisiae M40-10(KCTC 1030BP) which contains increased RNA content and obtained by mutation of Saccharomyces cerevisiae using EMS(Ethyl Methanesulfonate), and its mass-producing method by culturing it in a medium, such as CSL(corn steep liquor) and molasses. CONSTITUTION: The novel mutant strain, Saccharomyces cerevisiae M40-10(KCTC 1030BP) is prepared by treating Saccharomyces cerevisiae with a chemical mutagen, EMS; and selecting and separating a colony which has no viability in a medium containing metallic salt, KCl, inhibiting RNase activity. Therefore, the novel strain has increased RNA content.

Abstract translation: 目的：提供一种新型酿酒酵母M40-10（KCTC 1030BP），其含有增加的RNA含量并通过使用EMS（乙基甲磺酸盐）突变酿酒酵母而获得，并且通过在培养基如CSL中培养其批量生产方法 （玉米浆）和糖蜜。 构成：通过用化学诱变剂EMS处理酿酒酵母，制备新的突变株酿酒酵母M40-10（KCTC 1030BP） 并在含有金属盐KCl的培养基中选择并分离没有活力的菌落，从而抑制RNase活性。 因此，该新型菌株具有增加的RNA含量。

公开(公告)号：KR1020030018736A

公开(公告)日：2003-03-06

申请号：KR1020010053264

申请日：2001-08-31

Applicant: 주식회사농심

Inventor： 김재훈 ,  남희섭 ,  최인재 ,  남수완 ,  김재범

IPC: C12N1/16

Abstract: PURPOSE: Provided are a novel Saccharomyces cerevisiae M40-10(KCTC 1030BP) which contains increased RNA content and obtained by mutation of Saccharomyces cerevisiae using EMS(Ethyl Methanesulfonate), and its mass-producing method by culturing it in a medium, such as CSL(corn steep liquor) and molasses. CONSTITUTION: The novel mutant strain, Saccharomyces cerevisiae M40-10(KCTC 1030BP) is prepared by treating Saccharomyces cerevisiae with a chemical mutagen, EMS; and selecting and separating a colony which has no viability in a medium containing metallic salt, KCl, inhibiting RNase activity. Therefore, the novel strain has increased RNA content.

Abstract translation: 目的：提供一种新型酿酒酵母M40-10（KCTC 1030BP），其含有增加的RNA含量并通过使用EMS（乙基甲磺酸盐）的酿酒酵母突变获得，并且其批量生产方法通过在诸如CSL的培养基中培养 （玉米浆）和糖蜜。 构成：通过用化学诱变剂EMS处理酿酒酵母制备新型突变菌株，酿酒酵母M40-10（KCTC 1030BP）; 并且在含有金属盐的KCl的培养基中选择和分离不具有活性的菌落，抑制RNase活性。 因此，新菌株RNA含量增加。