公开(公告)号：KR102167336B1

公开(公告)日：2020-10-20

申请号：KR1020180141792

申请日：2018-11-16

Applicant: 충남대학교산학협력단 ,  김선혜

Inventor： 정원석 ,  채중령 ,  강지연 ,  설찬용 ,  김선혜 ,  최주원 ,  성석현

IPC: B01D39/14

公开(公告)号：KR102167314B1

公开(公告)日：2020-10-20

申请号：KR1020180141804

申请日：2018-11-16

Applicant: 충남대학교산학협력단 ,  김선혜

Inventor： 정원석 ,  채중령 ,  강지연 ,  설찬용 ,  김선혜 ,  최주원 ,  성석현

IPC: B01D39/14

公开(公告)号：KR1020020036981A

公开(公告)日：2002-05-17

申请号：KR1020020014640

申请日：2002-03-19

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  선남규 ,  김지연 ,  김소연 ,  이승현 ,  이용우 ,  이승희 ,  김선혜

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A process for preparing low molecular weight angiotensin converting enzyme(ACE) inhibitor from the extract of wild chrysanthemum is provided, thereby effectively and cheaply preparing the ACE inhibitor. CONSTITUTION: The process for preparing low molecular weight angiotensin converting enzyme(ACE) inhibitor from the extract of wild chrysanthemum comprises the steps of: washing wild chrysanthemum with water and extracting it using a reflux condenser at 100 deg.C for 2 hours; filtering and centrifuging at 4 deg.C and 8000 rpm for 30 minutes to obtain the supernatant; ultrafiltering the supernatant with molecular weight cut off(MWCO) of 1000 dalton to obtain a crude extract of wild chrysanthemum; and sequentially subjecting the crude extract of wild chrysanthemum to gel filtration chromatography, HPLC, ion exchange chromatography and reverse HPLC to obtain the low molecular weight angiotensin converting enzyme(ACE) inhibitor.

Abstract translation: 目的：提供从野菊花提取物制备低分子量血管紧张素转换酶（ACE）抑制剂的方法，从而有效且廉价地制备ACE抑制剂。 构成：从野菊花提取物制备低分子量血管紧张素转换酶（ACE）抑制剂的方法包括以下步骤：用水洗涤野菊花，并在100℃下使用回流冷凝器提取2小时; 过滤并在4℃和8000rpm下离心30分钟，得到上清液; 超分子量截留（MWCO）为1000道尔顿的上清液，以获得野菊花的粗提物; 并依次对野菊花提取物进行凝胶过滤层析，HPLC，离子交换层析和反相HPLC，得到低分子量血管紧张素转换酶（ACE）抑制剂。