公开(公告)号：KR100458965B1

公开(公告)日：2004-12-03

申请号：KR1020020011577

申请日：2002-03-05

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  이승환 ,  이승현 ,  선남규

IPC: A23L1/312

Abstract: PURPOSE: Provided is a manufacture of bovine and porcine plasma protein powders by treating low dose of gamma-ray, thereby preventing contamination by microorganisms, and removing biological risk factors while maintaining physicochemical properties of the plasma protein. CONSTITUTION: A manufacture of bovine and porcine plasma protein powders comprises the steps of: separating plasma from bovine and porcine using a centrifuger; precipitating the plasma using 2-10% trichloroacetic acid, 0.6-3N of hydrochloric acid, 50-70% of ammonium sulfate and the like; drying the precipitated plasma to give powder thereof; and packing the powder with polyethylene packing material and treating gamma-ray.

Abstract translation: 目的：提供一种通过处理低剂量的γ射线来制造牛和猪血浆蛋白质粉末，从而防止微生物污染，并且在保持血浆蛋白质的物理化学性质的同时去除生物危险因素。 组成：牛和猪血浆蛋白粉的生产包括以下步骤：用离心机从牛和猪中分离血浆; 使用2-10％三氯乙酸，0.6-3N盐酸，50-70％硫酸铵等沉淀血浆; 干燥沉淀的等离子体以得到其粉末; 并用聚乙烯包装材料包装粉末并处理γ射线。

公开(公告)号：KR1020030072044A

公开(公告)日：2003-09-13

申请号：KR1020020011577

申请日：2002-03-05

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  이승환 ,  이승현 ,  선남규

IPC: A23L1/312

Abstract: PURPOSE: Provided is a manufacture of bovine and porcine plasma protein powders by treating low dose of gamma-ray, thereby preventing contamination by microorganisms, and removing biological risk factors while maintaining physicochemical properties of the plasma protein. CONSTITUTION: A manufacture of bovine and porcine plasma protein powders comprises the steps of: separating plasma from bovine and porcine using a centrifuger; precipitating the plasma using 2-10% trichloroacetic acid, 0.6-3N of hydrochloric acid, 50-70% of ammonium sulfate and the like; drying the precipitated plasma to give powder thereof; and packing the powder with polyethylene packing material and treating gamma-ray.

Abstract translation: 目的：提供通过治疗低剂量γ射线的牛和猪血浆蛋白粉的制造，从而防止微生物的污染，并且在维持血浆蛋白质的物理化学性质的同时去除生物危险因素。 构成：牛和猪血浆蛋白粉的制造包括以下步骤：使用离心机从牛和猪分离血浆; 使用2-10％三氯乙酸，0.6-3N盐酸，50-70％硫酸铵等沉淀血浆; 干燥沉淀的等离子体，得到粉末; 并用聚乙烯包装材料包装粉末并处理γ射线。

公开(公告)号：KR1020010068159A

公开(公告)日：2001-07-13

申请号：KR1020010023416

申请日：2001-04-30

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  정혜영 ,  김숙현 ,  김지연 ,  선남규 ,  이진희 ,  이승환

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.

Abstract translation: 目的：提供莴苣根热水提取物中低分子量血管紧张素转换酶（ACE）抑制剂的制备方法，这是一个有效和经济的过程。 构成：莴苣根的低分子量ACE抑制剂的制造过程包括以下步骤：通过洗莴苣，分离根，向根中加入蒸馏水，并在100℃回流2小时提取，除去杂质。 ，60deg.C 过滤，离心30min。 在4℃，8000rpm下收集上层溶液; 使用超滤膜（M.W.的限制为1000）对上部溶液进行超滤，以制备提取物; 通过膜滤纸过滤，装载高效液相色谱法，分离出一个具有最高活性的级分F4; 然后使用反相HPLC收集F4，冷冻干燥和纯化。

公开(公告)号：KR100447534B1

公开(公告)日：2004-09-08

申请号：KR1020010023416

申请日：2001-04-30

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  원미선 ,  정혜영 ,  김숙현 ,  김지연 ,  선남규 ,  이진희 ,  이승환

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A manufacturing process for a low molecular weight of angiotensin converting enzyme(ACE) inhibitor from hot water extract of lettuce root is provided, which is an efficient and economical process. CONSTITUTION: The manufacturing process for a low molecular weight of ACE inhibitor from lettuce root comprises the steps of: removing an impurities by washing lettuce, separating its root, adding distilled water to the root, and extracting by making reflux for 2hrs at 100deg.C, 60deg.C; filtering, centrifuging for 30min. at 4deg.C, 8000rpm, and collecting the upper solution; ultra-filtering the upper solution using ultra-filter membrane(limit of M.W. is 1000) to prepare the extract; filtering through membrane filter paper, loading on high performance liquid chromatography, and separating a fraction, F4 with highest activity; and then gathering the F4, freeze-drying, and purifying using reversed phase HPLC.

Abstract translation: 目的：提供一种低分子量的生菜根热水提取物中的血管紧张素转化酶（ACE）抑制剂的制造工艺，这是一种高效且经济的工艺。 组成：低分子量生菜根ACE抑制剂的制造工艺包括以下步骤：通过洗涤生菜，分离其根，将蒸馏水加入根中，并通过在100℃回流2小时进行提取来除去杂质。 ，60deg.C; 过滤，离心30分钟。 在4℃，8000rpm下，收集上层溶液; 使用超滤膜（M.W.的极限为1000）超滤上层溶液以制备提取物; 通过膜滤纸过滤，上样于高效液相色谱仪，并分离出具有最高活性的馏分F4; 然后收集F4，冷冻干燥，并使用反相HPLC纯化。

公开(公告)号：KR1020020036981A

公开(公告)日：2002-05-17

申请号：KR1020020014640

申请日：2002-03-19

Applicant: 충남대학교산학협력단

Inventor： 송경빈 ,  선남규 ,  김지연 ,  김소연 ,  이승현 ,  이용우 ,  이승희 ,  김선혜

IPC: A61K36/28 ,  A61P9/12

Abstract: PURPOSE: A process for preparing low molecular weight angiotensin converting enzyme(ACE) inhibitor from the extract of wild chrysanthemum is provided, thereby effectively and cheaply preparing the ACE inhibitor. CONSTITUTION: The process for preparing low molecular weight angiotensin converting enzyme(ACE) inhibitor from the extract of wild chrysanthemum comprises the steps of: washing wild chrysanthemum with water and extracting it using a reflux condenser at 100 deg.C for 2 hours; filtering and centrifuging at 4 deg.C and 8000 rpm for 30 minutes to obtain the supernatant; ultrafiltering the supernatant with molecular weight cut off(MWCO) of 1000 dalton to obtain a crude extract of wild chrysanthemum; and sequentially subjecting the crude extract of wild chrysanthemum to gel filtration chromatography, HPLC, ion exchange chromatography and reverse HPLC to obtain the low molecular weight angiotensin converting enzyme(ACE) inhibitor.

Abstract translation: 目的：提供从野菊花提取物制备低分子量血管紧张素转换酶（ACE）抑制剂的方法，从而有效且廉价地制备ACE抑制剂。 构成：从野菊花提取物制备低分子量血管紧张素转换酶（ACE）抑制剂的方法包括以下步骤：用水洗涤野菊花，并在100℃下使用回流冷凝器提取2小时; 过滤并在4℃和8000rpm下离心30分钟，得到上清液; 超分子量截留（MWCO）为1000道尔顿的上清液，以获得野菊花的粗提物; 并依次对野菊花提取物进行凝胶过滤层析，HPLC，离子交换层析和反相HPLC，得到低分子量血管紧张素转换酶（ACE）抑制剂。