公开(公告)号：KR101111563B1

公开(公告)日：2012-06-18

申请号：KR1020090090588

申请日：2009-09-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  김혜란 ,  최수련 ,  홍창표 ,  방재욱 ,  진민아 ,  박범석

IPC: C12N15/65 ,  C12N15/29 ,  C12N15/11

Abstract: PURPOSE: A genetic map of Brassica rapa provided to improve the quality of Brassica rapa and to identify an useful gene which is used in protecting crops from diseases. CONSTITUTION: A kit for identifying SSR marker for constructing a genetic map of Brassica rapa contains a primer pair and reagent for PCR. The reagent contains thermoresistant DNA polymerase, dNTPs, and buffer. A method for identifying the SSR marker for constructing the genetic map for Brassica rapa comprises: a step of isolating genomic DNA from Brassica rapa; a step of amplifying a target sequence by PCR; and a step of detecting the PCR product. A method for selecting SSR primer set for constructing the genetic map comprises: a step of selecting BAC clone from base sequence of BAC clone; and a step of designing SSR primer set for 1-3 genetic maps per BAC clone from flaking base sequence of SSR motif.

公开(公告)号：KR1020110032866A

公开(公告)日：2011-03-30

申请号：KR1020090090588

申请日：2009-09-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  김혜란 ,  최수련 ,  홍창표 ,  방재욱 ,  진민아 ,  박범석

IPC: C12N15/65 ,  C12N15/29 ,  C12N15/11

Abstract: PURPOSE: A genetic map of Brassica rapa provided to improve the quality of Brassica rapa and to identify an useful gene which is used in protecting crops from diseases. CONSTITUTION: A kit for identifying SSR marker for constructing a genetic map of Brassica rapa contains a primer pair and reagent for PCR. The reagent contains thermoresistant DNA polymerase, dNTPs, and buffer. A method for identifying the SSR marker for constructing the genetic map for Brassica rapa comprises: a step of isolating genomic DNA from Brassica rapa; a step of amplifying a target sequence by PCR; and a step of detecting the PCR product. A method for selecting SSR primer set for constructing the genetic map comprises: a step of selecting BAC clone from base sequence of BAC clone; and a step of designing SSR primer set for 1-3 genetic maps per BAC clone from flaking base sequence of SSR motif.

Abstract translation: 目的：提供芸苔属的遗传图谱，以提高芸苔属的品质，并确定用于保护作物免受疾病的有用基因。 构成：用于鉴定构建芸苔属遗传图谱的SSR标记的试剂盒含有PCR引物对和试剂。 试剂含有耐热DNA聚合酶，dNTP和缓冲液。 鉴定用于构建芸苔属芸苔遗传图谱的SSR标记物的方法包括：从芸苔属油菜中分离基因组DNA的步骤; 通过PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。 一种选择用于构建遗传图谱的SSR引物组的方法，包括：从BAC克隆的碱基序列中选择BAC克隆的步骤; 以及从SSR基序的片状碱基序列设计每个BAC克隆的1-3个遗传图谱的SSR引物组的步骤。