公开(公告)号：KR1020100038258A

公开(公告)日：2010-04-14

申请号：KR1020080097442

申请日：2008-10-04

Applicant: 충남대학교산학협력단

Inventor： 송규용 ,  서상희 ,  김영호 ,  박용진 ,  박천정

IPC: A61K36/258 ,  A61P31/16

Abstract: PURPOSE: A linear double strand RNA molecule reducing expression of different target genes is provided to induce gene silence regardless of inversion orientation of siRNA. CONSTITUTION: A linear double strand RNA molecule contains 2-10 short interference RNAs(siRNA) which are serially connected. The siRNA comprises 18-24 nucleotides. The linear double strand RNA molecule contains two successive siRNAs. Each strand contains two 3'-terminal nucleotides which is not formed in base pair. A recombinant expression vector comprises double strand DNA sequence expressing the linear double strand RNA molecule. The expression of a target gene is suppressed by introducing the recombinant expression vector and linear type double strand RNA molecule in cells.

Abstract translation: 目的：提供减少不同靶基因表达的线性双链RNA分子，以诱导基因沉默，而不管siRNA的倒置方向如何。 构成：线性双链RNA分子含有2-10个短连接RNA（siRNA），它们连接在一起。 siRNA包含18-24个核苷酸。 线性双链RNA分子含有两个连续的siRNA。 每条链含有两个未在碱基对中形成的3'末端核苷酸。 重组表达载体包含表达线性双链RNA分子的双链DNA序列。 通过在细胞中引入重组表达载体和线型双链RNA分子来抑制靶基因的表达。