公开(公告)号：KR1020180094188A

公开(公告)日：2018-08-23

申请号：KR1020170019783

申请日：2017-02-14

Applicant: 충남대학교산학협력단 ,  대한민국(농촌진흥청장) ,  경운대학교 산학협력단

Inventor： 권오유 ,  최지영 ,  권기상 ,  이정훈

IPC: G01N1/40 ,  C07K1/14 ,  C07K1/34

CPC classification number: G01N1/4077 ,  C07K1/14 ,  C07K1/34 ,  G01N2001/4088

Abstract: 본발명은귀뚜라미혈림프를대량으로분리하는방법에관한것으로, 혈림프분리과정중에산화를방지하면서, 대량으로혈림프를분리함으로써귀뚜라미의질병진단, 신약개발및 새로운식품재료개발을위한연구에필요한혈림프를확보할수 있을것으로기대된다.

公开(公告)号：KR101911398B1

公开(公告)日：2018-10-25

申请号：KR1020170019783

申请日：2017-02-14

Applicant: 충남대학교산학협력단 ,  대한민국(농촌진흥청장) ,  경운대학교 산학협력단

Inventor： 권오유 ,  최지영 ,  권기상 ,  이정훈

IPC: G01N1/40 ,  C07K1/14 ,  C07K1/34

Abstract: 본발명은귀뚜라미혈림프를대량으로분리하는방법에관한것으로, 혈림프분리과정중에산화를방지하면서, 대량으로혈림프를분리함으로써귀뚜라미의질병진단, 신약개발및 새로운식품재료개발을위한연구에필요한혈림프를확보할수 있을것으로기대된다.

公开(公告)号：KR101679406B1

公开(公告)日：2016-11-24

申请号：KR1020150123982

申请日：2015-09-02

Applicant: 충남대학교산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 권오유 ,  최지영 ,  권기상 ,  김홍근 ,  박관호 ,  서현우

IPC: C12Q1/68 ,  C12N15/10 ,  C07K1/34 ,  G01N1/40

Abstract: 본발명은곤충의질병진단을위해혈림프조성물을제조하고, 이로부터엑소좀을분리하고, 엑소좀에존재하는생체분자를분리하는방법에관한것으로서, 상기곤충질병진단용혈림프조성물로부터엑소좀을분리하고, 분리한엑소좀으로부터질병과관련된단백질및 핵산을포함하는생체분자들을분리할수 있다. 분리한생체분자들을이용해곤충의질병진단에활용함으로써곤충의대량사육시 질병으로인한대규모의피해를줄임으로써곤충사육농가의안정적인수입원확보에기여할수 있다.

公开(公告)号：KR1020090072683A

公开(公告)日：2009-07-02

申请号：KR1020070140875

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09

Abstract: An elongation factor-1 alpha promoter of a silk worm is provided to increase the expression of foreign gene and massively produce recombinant protein. An elongation factor-1 alpha gene sequence of the sequence number 1 comprises gene area and promoter of the elongation factor-1 alpha gene. A cDNA sequence of elongation factor-1 alpha gene is denoted the sequence 2. The amino acid sequence denoted by the sequence number 3 is deduced. The maximum promoter activation area of elongation factor-1 alpha gene comprises 1-1542 sequences.

Abstract translation: 提供丝虫的延伸因子-1α启动子以增加外源基因的表达并大量生产重组蛋白。 序列号1的延伸因子-1α基因序列包含延伸因子-1α基因的基因区域和启动子。 延伸因子-1α基因的cDNA序列被表示为序列2.由序列号3表示的氨基酸序列被推导出。 延伸因子-1α基因的最大启动子活化区域包含1-1542个序列。

公开(公告)号：KR1020070060439A

公开(公告)日：2007-06-13

申请号：KR1020050119819

申请日：2005-12-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 윤은영 ,  구태원 ,  황재삼 ,  최광호 ,  강석우 ,  권오유

IPC: C12N15/63 ,  C12N15/10

Abstract: A transformed insect cell containing human beta-1,4-galactosyltransferase and rat alpha-2,6-sialyltransferase and having an enhanced sugar chain addition function and a production method of glycoproteins from the same transformed insect cells are provided to improve production stability and economic efficiency of useful sugar proteins derived from human by using inexpensive insect cells. The transformed insect cell containing human beta-1,4-galactosyltransferase and rat alpha-2,6-sialyltransferase and having the enhanced sugar chain addition function is produced by transforming an insect cell line Sf9 with a recombinant vector containing the human beta-1,4-galactosyltransferase gene and rat alpha-2,6-sialyltransferase gene. The glycoproteins which are added sialic acid or sialic acid and galactose are produced by culturing the transformed insect cell.

Abstract translation: 提供含有人β-1,4-半乳糖基转移酶和大鼠α-2,6-唾液酸转移酶的转化的昆虫细胞，并且具有增强的糖链添加功能和来自相同转化的昆虫细胞的糖蛋白的制备方法以提高生产稳定性和经济性 通过使用便宜的昆虫细胞从人类衍生的有用糖蛋白的效率。 通过用包含人β-1的重组载体转化昆虫细胞系Sf9来产生含有人β-1,4-半乳糖基转移酶和大鼠α-2,6-唾液酸转移酶并具有增强的糖链加成功能的转化的昆虫细胞， 4-半乳糖基转移酶基因和大鼠α-2,6-唾液酸转移酶基因。 通过培养转化的昆虫细胞产生加入唾液酸或唾液酸和半乳糖的糖蛋白。

公开(公告)号：KR100414182B1

公开(公告)日：2004-01-07

申请号：KR1020010039221

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  황재삼 ,  강석우 ,  권오유

IPC: C12N15/10

Abstract: PURPOSE: Provided are a nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, and an amino acid sequence thereof, thereby increasing the expression of useful proteins and consequently mass-producing them economically. CONSTITUTION: The nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, is represented by the SEQ ID NO:1, and its amino acid sequence is represented by the SEQ ID NO:2.

Abstract translation: 目的：提供从家蚕的培养细胞中分离的编码蛋白质二硫键异构酶（PDI）的cDNA的核苷酸序列及其氨基酸序列，由此增加有用蛋白质的表达，并因此经济地批量生产它们。 构成：从家蚕的培养细胞分离的编码蛋白质二硫键异构酶（PDI）的cDNA的核苷酸序列由SEQ ID NO：1表示，其氨基酸序列由SEQ ID NO：2表示。

公开(公告)号：KR100918016B1

公开(公告)日：2009-09-24

申请号：KR1020070140875

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09

Abstract: 본 발명은 누에로부터 분리한 연장인자-1 알파 유전자의 전체 게놈 유전자 서열 및 최대 프로모터 활성 부위에 관한 것이다.
누에, 프로모터, 형질전환운반체, 액틴3, 연장인자-1 알파, 루시퍼레이즈, piggyBac

公开(公告)号：KR1020080059702A

公开(公告)日：2008-07-01

申请号：KR1020060133334

申请日：2006-12-26

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  손봉희 ,  강석우 ,  김성완 ,  권오유

IPC: C12N15/33 ,  C12N15/63 ,  C12N15/10

Abstract: A hr3(homologous region 3) enhancer isolated from Bombyx mori nucleopolyhedrovirus K1 is provided to maximize the activity of promoter and produce a large quantity of a recombinant protein by optimizing the location and direction of the enhancer in the expression vector. A hr3 enhancer isolated from Bombyx mori nucleopolyhedrovirus K1 has the nucleotide sequence of SEQ ID NO:1. The mass-production of a recombinant protein is accomplished by locating the hr3 enhancer of SEQ ID NO:1 in a site downstream of the SV40 poly A site in 3' to 5' direction in a protein expression vector.

Abstract translation: 提供从家蚕核多角体病毒K1分离的hr3（同源区3）增强子，以通过优化表达载体中增强子的位置和方向来最大化启动子的活性并产生大量的重组蛋白。 从家蚕核多角体病毒K1分离的hr3增强子具有SEQ ID NO：1的核苷酸序列。 通过将SEQ ID NO：1的hr3增强子定位在蛋白质表达载体中3'至5'方向的SV40聚A位点下游的位点来实现大量生产重组蛋白。

公开(公告)号：KR1020090072684A

公开(公告)日：2009-07-02

申请号：KR1020070140876

申请日：2007-12-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  최광호 ,  김용보 ,  강석우 ,  강민욱 ,  권오유

IPC: C12N15/31 ,  C07K14/315

Abstract: A promoter and entire genome sequence of heat shock protein isolated from a silk worm is provided to express a excess amount foreign gene and easily select transformant of silk worm. A gene sequence of heat shock protein 70 gene denoted by the sequence 1 comprises gene area and promoter area of heat shock protein 70 isolated silk worm. The Cdna sequence of heat shock protein 70 is denoted by the sequence 2. The amino acid sequence denoted by the sequenc 3 is deduced by cDNA sequence. The maximum promoter activation area of heat shock protein 70 gene comprises 367-1517 sequences.

Abstract translation: 提供从丝虫分离的热休克蛋白的启动子和全基因组序列，以表达过量的外源基因，并容易选择蚕丝的转化体。 由序列1表示的热休克蛋白70基因的基因序列包含热休克蛋白70分离的丝虫的基因面积和启动子面积。 热休克蛋白70的Cdna序列由序列2表示。由序列3表示的氨基酸序列由cDNA序列推导出。 热休克蛋白70基因的最大启动子活化区域包含367-1517个序列。

公开(公告)号：KR1020030002924A

公开(公告)日：2003-01-09

申请号：KR1020010039221

申请日：2001-07-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 구태원 ,  윤은영 ,  황재삼 ,  강석우 ,  권오유

IPC: C12N15/10

Abstract: PURPOSE: Provided are a nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, and an amino acid sequence thereof, thereby increasing the expression of useful proteins and consequently mass-producing them economically. CONSTITUTION: The nucleotide sequence of cDNA coding protein disulfide isomerase(PDI) isolated from the cultured cell of a silkworm, is represented by the SEQ ID NO:1, and its amino acid sequence is represented by the SEQ ID NO:2.

Abstract translation: 目的：提供从蚕的培养细胞分离的cDNA编码蛋白质二硫键异构酶（PDI）的核苷酸序列及其氨基酸序列，从而增加有用蛋白质的表达，从而经济地批量生产它们。 构成：从蚕的培养细胞分离的cDNA编码蛋白质二硫键异构酶（PDI）的核苷酸序列由SEQ ID NO：1表示，其氨基酸序列由SEQ ID NO：2表示。