핵산 추출 방법
    1.
    发明授权
    핵산 추출 방법 有权
    核酸提取方法

    公开(公告)号:KR101006562B1

    公开(公告)日:2011-01-07

    申请号:KR1020080061903

    申请日:2008-06-27

    CPC classification number: C12N15/1017 C12N15/1006

    Abstract: 본 발명은 핵산 추출 방법에 관한 것으로서, 관 형상 튜브 내에 하이드로겔 컬럼을 형성하는 하이드로겔 컬럼 제작 단계와, 세포를 파괴하여 세포 용해물을 얻는 세포 용해물 제작 단계와, 핵산을 하이드로겔 컬럼을 통과하여 배출시키는 핵산 필터링 단계, 및 하이드로겔 컬럼을 통과한 핵산을 외부로 추출하는 핵산 추출 단계를 포함할 수 있다.
    핵산, 단백질, 원심분리, 하이드로겔, 아가로스겔

    핵산 추출 장치
    3.
    发明公开
    핵산 추출 장치 失效
    核酸提取装置

    公开(公告)号:KR1020100001826A

    公开(公告)日:2010-01-06

    申请号:KR1020080061902

    申请日:2008-06-27

    Abstract: PURPOSE: An apparatus for extracting nucleic acid using hydrogel column is provided to extract nucleic acid withough impurity and improve nucleic acid yield. CONSTITUTION: An apparatus for extracting nucleic acid comprises a tube having an outlet opened at one side and a hydrogel support(16) filtering impurities. The apparatus further comprises a housing(12) connected to the outlet. The hydrogel support is made of 1%-2% of agarose gel. The hydrogel support has an injection groove connected in a longitudinal direction. The injection groove is formed at the center of the hydrogel support.

    Abstract translation: 目的:提供使用水凝胶柱提取核酸的装置,以提取不含杂质的核酸,提高核酸产量。 构成:用于提取核酸的装置包括具有在一侧开口的出口的管和用于过滤杂质的水凝胶载体(16)。 该装置还包括连接到出口的壳体(12)。 水凝胶载体由1%-2%的琼脂糖凝胶制成。 水凝胶支撑体具有沿纵向方向连接的注入槽。 注入槽形成在水凝胶支撑体的中心。

    핵산 추출 방법
    4.
    发明公开
    핵산 추출 방법 有权
    核酸提取方法

    公开(公告)号:KR1020100001827A

    公开(公告)日:2010-01-06

    申请号:KR1020080061903

    申请日:2008-06-27

    CPC classification number: C12N15/1017 C12N15/1006 C12M33/04 C12M23/06

    Abstract: PURPOSE: A method for isolating nucleic acid is provided to improve nucleic acid yield and reduce damage to the nucleic acid. CONSTITUTION: A method for isolating nucleic acid comprises: a step of making hydrogel column in a tube; a step of crushing cell to obtain cell lysate; a step of passing cell lysate through hydrogel column; and a step of isolating nucleic acid. The hydrogel column is agarose gel containing 1-2% of agarose. The method further comprises a step of adding lysis buffer and proteinase K.

    Abstract translation: 目的:提供分离核酸的方法以改善核酸产量并减少对核酸的损伤。 构成:分离核酸的方法包括:在管中制备水凝胶柱的步骤; 破碎细胞以获得细胞裂解物的步骤; 将细胞裂解物通过水凝胶柱的步骤; 和分离核酸的步骤。 水凝胶柱是含有1-2%琼脂糖的琼脂糖凝胶。 该方法还包括加入裂解缓冲液和蛋白酶K.

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