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公开(公告)号：KR100262818B1
公开(公告)日：2000-08-01
申请号：KR1019970075846
申请日：1997-12-29
Applicant: 한국과학기술연구원
Abstract: PURPOSE: Provided is a recombinant plasmid, pAc/18L1-L2, which simultaneously expresses L1 and L2 genes of capsid proteins of Human Papillomavirus(HPV) 18 type in an insect cell. Also, a recombinant baculovirus pAc/18L1-L2 including this recombinant plasmid is provided, which produces Human Papillomavirus 18 type in a large quantity to help the development of preventive vaccine against Human Papillomavirus 18 type. CONSTITUTION: A method for preparation of pseudo Human Papillomavirus 18 type is composed of the following steps of: preparing a recombinant expression vector pAc/18L1-L2 which produces capsid proteins, L1 and L2 of HPV 18 type; transforming Sf-21 insect cells with the recombinant plasmid and baculovirus DNA to prepare a recombinant baculovirus pAc/18L1-L2(KCTC 0414BP); culturing and separating it to obtain Human Papillomavirus 18 type like particle.
Abstract translation: 目的：提供一种重组质粒pAc / 18L1-L2，同时在昆虫细胞中表达人乳头瘤病毒（HPV）18型衣壳蛋白的L1和L2基因。 此外，提供了包含该重组质粒的重组杆状病毒pAc / 18L1-L2，其大量产生人乳头瘤病毒18型，以帮助开发针对人乳头状瘤病毒18型的预防性疫苗。 构成：拟人类乳头瘤病毒18型的制备方法由以下步骤组成：制备重组表达载体pAc / 18L1-L2，其产生HPV18型衣壳蛋白L1和L2; 用重组质粒和杆状病毒DNA转化Sf-21昆虫细胞，制备重组杆状病毒pAc / 18L1-L2（KCTC 0414BP）; 培养和分离以获得人乳头状瘤病毒18型样颗粒。
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公开(公告)号：KR1019970043048A
公开(公告)日：1997-07-26
申请号：KR1019950055519
申请日：1995-12-23
Abstract: 본 발명은 사람 파필로마바이러스 18형 E7 유전자를 함유하는 재조합 플라스미드에 의해 형질전환되어 가용성 18형 E7 재조합 단백질을 고효율로 생산할 수 있는 대장균 형질전환체, 대장균 형질전환체를 이용하여 비융합형이고 가용성인 18형 E7재조합 단백질을 제조하는 방법 및 상기 재조합 단백질에 대해 특이적인 폴리클로날 항체에 관한 것이다.
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公开(公告)号：KR1020000000844A
公开(公告)日：2000-01-15
申请号：KR1019980020709
申请日：1998-06-03
Applicant: 한국과학기술연구원
CPC classification number: C12N15/866 , A61K38/162 , A61K39/12
Abstract: PURPOSE: A method of delivering genes into various liver cancer cells using virus-like particles (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: A method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex with polyoxyethylene-polyoxypropylene block copolymer before transfecting into liver cancer cell line.
Abstract translation: 目的：提供使用病毒样颗粒（VLP）将基因递送到各种肝癌细胞中的方法，以通过将基因与VLP结合然后用聚氧乙烯 - 聚氧丙烯嵌段共聚物进行处理来提高递送效率。 构成：一种方法包括以下步骤：通过将乳头瘤病毒衣壳抗原或其他病毒衣壳抗原感染到昆虫细胞中而获得的重组蛋白中产生VLP; 通过将聚赖氨酸与VLP缀合产生VLP-PL缀合物; 分别通过连接质粒pCMVβ-gal，pSV2CAT，pCMV-p53获得VLP-PL-pCMVβ-gal质粒复合物，VLP-PL-pSV2CAT质粒复合物或VLP-PL-pCMV-p53质粒复合物; 在转染肝癌细胞株前用聚氧乙烯 - 聚氧丙烯嵌段共聚物处理复合物。
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公开(公告)号：KR100209295B1
公开(公告)日：1999-07-15
申请号：KR1019960038373
申请日：1996-09-05
IPC: C07K7/06
Abstract: 본 발명은 사람 파필로마바이러스 16형 E6 단백질 항원에 대한 세포독성 T 임파구(cytotoxic T lymphocyte, CTL)를 유도하거나 이 바이러스에 대한 인체의 면역성을 조절할 수 있는 신규한 펩타이드에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR100184715B1
公开(公告)日：1999-05-01
申请号：KR1019950055521
申请日：1995-12-23
Abstract: 본 발명은 동물세포내에서 사람 파필로마바이러스 18형 E7 재조합 단백질을 발현시킬 수 있는 형질전환체 및 사람 파필로마바이러스 18형 E7 재조합 단백질을 유효성분으로 함유함을 특징으로 하여 이 바이러스에 의해 감염된 세포를 특이적으로 괴사시키는 자궁암 면역치료제 조성물에 관한 것이다. 본 발명은 또한, 상기 재조합 단백질을 유효성분으로 함유하는 자궁암 예방백신에 관한 것이다.
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公开(公告)号：KR1019970009811A
公开(公告)日：1997-03-27
申请号：KR1019950024888
申请日：1995-08-12
Applicant: 한국과학기술연구원
IPC: A61K38/00
Abstract: 본 발명은 인체 파필로마바이러스 16형 E6 단백질을 포함하는 자궁암 면역치료제에 관한 것으로, 인체 파필로마바이러스 16형 E6 단백질은 항원으로 사용되어 항체 반응 및 면역 반응을 유도하고 암세포 살해능을 갖는 면역 세포를 활성화시켜 암세포를 효과적으로 살해하므로 자궁암을 치료하는데 유용하게 이용될 수 있다.
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公开(公告)号：KR100345250B1
公开(公告)日：2003-01-15
申请号：KR1019980020709
申请日：1998-06-03
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method of delivering genes into various liver cancer cells using virus-like particles (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: A method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex with polyoxyethylene-polyoxypropylene block copolymer before transfecting into liver cancer cell line.
Abstract translation: 目的：提供使用病毒样颗粒（VLP）将基因递送至各种肝癌细胞的方法，以通过将基因结合至VLP然后用聚氧乙烯 - 聚氧丙烯嵌段共聚物处理来提高递送效率。 构成：一种方法包括以下步骤：从通过将乳头瘤病毒衣壳抗原或其他病毒衣壳抗原感染到昆虫细胞中获得的重组蛋白产生VLP; 通过将聚赖氨酸与VLP缀合产生VLP-PL缀合物; 通过分别连接质粒pCMVβ-gal，pSV2CAT，pCMV-p53获得VLP-PL-pCMVβ-gal质粒复合物，VLP-PL-pSV2CAT质粒复合物或VLP-PL-pCMV- 在转染入肝癌细胞系之前用聚氧乙烯 - 聚氧丙烯嵌段共聚物处理复合物。
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公开(公告)号：KR100345249B1
公开(公告)日：2003-01-15
申请号：KR1019980021123
申请日：1998-06-08
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method of delivering genes into various uterine cancer cells using a virus-like particle (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: The method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex polyoxyethylene-polyoxypropylene block copolymer before transfecting into uterine cancer cell line.
Abstract translation: 目的：提供使用病毒样颗粒（VLP）将基因递送至各种子宫癌细胞的方法，以通过将基因与VLP结合然后用聚氧乙烯 - 聚氧丙烯嵌段共聚物处理来提高递送效率。 构成：该方法包括以下步骤：从感染乳头瘤病毒衣壳抗原或其他病毒衣壳抗原到昆虫细胞中获得的重组蛋白产生VLP; 通过将聚赖氨酸与VLP缀合产生VLP-PL缀合物; 通过分别连接质粒pCMVβ-gal，pSV2CAT，pCMV-p53获得VLP-PL-pCMVβ-gal质粒复合物，VLP-PL-pSV2CAT质粒复合物或VLP-PL-pCMV- 在转染入子宫癌细胞系之前处理复合聚氧乙烯 - 聚氧丙烯嵌段共聚物。
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公开(公告)号：KR1020000001077A
公开(公告)日：2000-01-15
申请号：KR1019980021123
申请日：1998-06-08
Applicant: 한국과학기술연구원
CPC classification number: C12N15/866 , A61K38/162
Abstract: PURPOSE: A method of delivering genes into various uterine cancer cells using a virus-like particle (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: The method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex polyoxyethylene-polyoxypropylene block copolymer before transfecting into uterine cancer cell line.
Abstract translation: 目的：提供使用病毒样颗粒（VLP）将基因递送到各种子宫癌细胞的方法，以通过将基因与VLP结合然后用聚氧乙烯 - 聚氧丙烯嵌段共聚物进行处理来提高递送效率。 构成：该方法包括以下步骤：通过将乳头瘤病毒衣壳抗原或其他病毒衣壳抗原感染昆虫细胞获得的重组蛋白产生VLP; 通过将聚赖氨酸与VLP缀合产生VLP-PL缀合物; 分别通过连接质粒pCMVβ-gal，pSV2CAT，pCMV-p53获得VLP-PL-pCMVβ-gal质粒复合物，VLP-PL-pSV2CAT质粒复合物或VLP-PL-pCMV-p53质粒复合物; 在转染子宫癌细胞株前处理复合的聚氧乙烯 - 聚氧丙烯嵌段共聚物。
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