公开(公告)号：KR102015360B1

公开(公告)日：2019-08-28

申请号：KR1020170003826

申请日：2017-01-10

Applicant: 광주과학기술원 ,  한국과학기술연구원 ,  건국대학교 산학협력단

Inventor： 김민곤 ,  이석 ,  송창선 ,  김상경 ,  한겨레

IPC: G01N33/543 ,  G01N33/53

公开(公告)号：KR1019990065396A

公开(公告)日：1999-08-05

申请号：KR1019980000669

申请日：1998-01-13

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  김철호 ,  송기방 ,  김민곤 ,  서정우 ,  정봉현

IPC: C12P19/02

Abstract: 본 발명은 라넬라 아쿠아틸리스 유래의 레반수크라제를 이용하여 설탕과 알콜로부터 알킬 베타-디-프럭토시드를 생산하는 방법에 관한 것이다.
본 발명은 메틸렌, 에틸렌, 글리세롤, 에틸렌글리콜, 프로필렌글리콜과 같은여러 알콜을 사용하여 생산되는 알킬 베타-디-프럭토시드의 종류를 다양하였다. 또 알콜농도는 높히고 반응 온도는 저온으로 유지하여 생산량도 향상시켰다.

公开(公告)号：KR1020000056647A

公开(公告)日：2000-09-15

申请号：KR1019990006137

申请日：1999-02-24

Applicant: 한국과학기술연구원

Inventor： 정봉현 ,  김민곤 ,  김철호 ,  최순자 ,  이상기

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C07K1/14

Abstract: PURPOSE: Lipase purified with an organic solvent, its preparation method and chiral medical and pharmaceutical products using the lipase are provided, which lipase is improved in the enantiomeric ratio and is useful to prepare optically pure (S)-ketoprofen, (S)-ibuprofen and (S)-suprofen. CONSTITUTION: A lipase with improved enantiomeric ratio is prepared by adding 0.3-1.0 times by volume of an organic solvent to Candida rugosa lipase solution to collect the first-stage precipitate; adding 0.3-1.0 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the second-stage precipitate; and 0.8-1.2 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the precipitate. The organic solvent is acetone or isopropane.

Abstract translation: 目的：提供用有机溶剂纯化的脂肪酶，其制备方法和使用脂肪酶的手性医药产品，该脂肪酶的对映体比例得到改善，可用于制备光学纯的（S） - 酮洛芬，（S） - 布洛芬 和（S） - 布洛芬。 构成：通过向假丝酵母脂肪酶溶液中加入0.3-1.0倍体积的有机溶剂来收集第一阶段沉淀物来制备具有改善的对映异构体比例的脂肪酶; 将基于原始脂肪酶溶液的体积的有机溶剂的0.3-1.0倍体积添加到上清液中，以收集第二阶段沉淀物; 和0.8-1.2倍体积的有机溶剂，基于原始脂肪酶溶液到上清液的体积，收集沉淀物。 有机溶剂为丙酮或异丙醇。

公开(公告)号：KR100320270B1

公开(公告)日：2002-01-10

申请号：KR1019990006137

申请日：1999-02-24

Applicant: 한국과학기술연구원

Inventor： 정봉현 ,  김민곤 ,  김철호 ,  최순자 ,  이상기

IPC: C12N9/20

Abstract: 본발명은유기용매로정제한리파제효소및 이를이용한키랄의약품의제조방법에관한것으로캔디다루고사유래의리파제효소를유기용매침전에의해부분정제하여광학분활반응에대해선택성이높은효소를제공하고상기광학분활반응성에대해선택성이높은효소는가수분해반응에의해광학적으로순수한비스토로이드계소염진통제 (S)-케토프로펜, (S)-이뷰프로펜, (S)-슈프로펜을생산하는뛰어난효과가있다.

公开(公告)号：KR100257118B1

公开(公告)日：2000-05-15

申请号：KR1019980000669

申请日：1998-01-13

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  김철호 ,  송기방 ,  김민곤 ,  서정우 ,  정봉현

IPC: C12P19/02

Abstract: PURPOSE: A method for producing alkyl beta-di-fructoside using levansucrase is provided, thereby the novel alkyl beta-di-fructoside can be produced in higher yield. CONSTITUTION: The alkyl beta-di-fructoside is similar to alkyl glucoside which is used for drugs, foods and cosmetics as sugar surfactant and represented by formula (I), in which R is methyl, ethyl, glycerol, ethyleneglycol or propyleneglycol. The alkyl beta-di-fructoside is produced by the steps of: adjusting the hydrogen concentration of reaction solution containing 20 to 80%(v/v) of alcohol to pH 5.8 to 6.2 with MES buffer solution; and reacting 50 to 100 g/l of sugar with levansucrase in the reaction solution at 10 to 20 deg. C, in which the alcohol is selected from the group consisting of methanol, ethanol, ethyleneglycol, propyleneglycol and glycerol.

Abstract translation: 目的：提供一种使用左曲聚糖酶制备β-二氢蝶啶糖苷的方法，从而可以更高的产率生产新的β-二氢果糖苷。 构成：β-二糖果糖苷类似于烷基葡糖苷，其用作药物，食品和化妆品作为糖表面活性剂并由式（I）表示，其中R是甲基，乙基，甘油，乙二醇或丙二醇。 通过以下步骤制备烷基β-二果糖苷：通过MES缓冲液将含有20-80％（v / v）醇的反应溶液的氢浓度调节至pH5.8至6.2; 并在反应溶液中在10至20℃下使50至100g / l的糖与左甘蔗脂酶反应。 C，其中醇选自甲醇，乙醇，乙二醇，丙二醇和甘油。