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公开(公告)号：KR1020000056647A
公开(公告)日：2000-09-15
申请号：KR1019990006137
申请日：1999-02-24
Applicant: 한국과학기술연구원
IPC: C12N9/20
Abstract: PURPOSE: Lipase purified with an organic solvent, its preparation method and chiral medical and pharmaceutical products using the lipase are provided, which lipase is improved in the enantiomeric ratio and is useful to prepare optically pure (S)-ketoprofen, (S)-ibuprofen and (S)-suprofen. CONSTITUTION: A lipase with improved enantiomeric ratio is prepared by adding 0.3-1.0 times by volume of an organic solvent to Candida rugosa lipase solution to collect the first-stage precipitate; adding 0.3-1.0 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the second-stage precipitate; and 0.8-1.2 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the precipitate. The organic solvent is acetone or isopropane.
Abstract translation: 目的：提供用有机溶剂纯化的脂肪酶，其制备方法和使用脂肪酶的手性医药产品，该脂肪酶的对映体比例得到改善，可用于制备光学纯的（S） - 酮洛芬，（S） - 布洛芬 和（S） - 布洛芬。 构成：通过向假丝酵母脂肪酶溶液中加入0.3-1.0倍体积的有机溶剂来收集第一阶段沉淀物来制备具有改善的对映异构体比例的脂肪酶; 将基于原始脂肪酶溶液的体积的有机溶剂的0.3-1.0倍体积添加到上清液中，以收集第二阶段沉淀物; 和0.8-1.2倍体积的有机溶剂，基于原始脂肪酶溶液到上清液的体积，收集沉淀物。 有机溶剂为丙酮或异丙醇。
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Patent Agency Ranking
公开(公告)号：KR100252194B1
公开(公告)日：2000-04-15
申请号：KR1019970051930
申请日：1997-10-10
Applicant: 한국과학기술연구원
IPC: A61K31/732 , A61K36/232
CPC classification number: C08B37/0045 , A61K31/715 , C08B37/00
Abstract: PURPOSE: A novel pectin polysaccharides(Angellan-I, Angellan-II) separated from Angelica gigas Nakai is provided, which contains galacturonic acid, arabinose and galactose, therefore shows immune-increasing effect and also can be used as an immune-increasing reagent in the basic study for immunology. CONSTITUTION: A process for the separation of novel pectin polysaccharides(Angellan-I, Angellan-II) from Angelica gigas Nakai comprises the steps of: pulverizing Angelica gigas Nakai root into pieces, adding water and extracting while circulating at 100deg.C for 1hr; adding 3times of ethanol to the residual solution, leaving at 4deg.C for 3hrs and centrifuging to get extracted precipitates; dissolving the ethanol extract in water to remove impurities, adding 3times of ethanol to the residual solution again, centrifuging and processing dialysis; freeze-drying the reagent to get crude polysaccharides; and separating the crude polysaccharides into acidic polysaccharides(Angellan I) and neutral polysaccharides(Angellan II) by dissolving, washing, centrifuging, doing dialysis and freeze-drying.
Abstract translation: 目的：提供从当归纳西分离的新型果胶多糖（Angellan-I，Angellan-II），其含有半乳糖醛酸，阿拉伯糖和半乳糖，因此具有免疫增加作用，并且还可用作免疫增加试剂 免疫学的基础研究。 构成：从当归Nakai中分离出新型果胶多糖（Angellan-I，Angellan-II）的方法，包括以下步骤：将当归Nakai根粉碎成片，加水并在100℃循环提取1小时; 向剩余溶液中加入3倍的乙醇，在4℃下放置3h，离心得到提取的沉淀物; 将乙醇提取液溶解于水中去除杂质，再次向剩余溶液中加入3倍乙醇，离心和加工透析液; 冷冻干燥试剂得到粗多糖; 并通过溶解，洗涤，离心，进行透析和冷冻干燥将粗多糖分离成酸性多糖（Angellan I）和中性多糖（Angellan II）。
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公开(公告)号：KR1019990031277A
公开(公告)日：1999-05-06
申请号：KR1019970051930
申请日：1997-10-10
Applicant: 한국과학기술연구원
IPC: A61K31/732 , A61K36/232
Abstract: 본 발명은 참당귀(Angelica gigas Nakai)로 부터 분리한 펙틴질 다당인 안젤란-I과 안젤란-II 등의 물질, 이들의 순수분리방법 및 이들의 항암제 또는 면역증강제로서의 용도에 관한 것이다. 더욱 상세하게는 참당귀에서 분리한 갈락트론산, 아라비노스, 그리고 갈락토스 등을 함유하는 면역증강제의 조성물 이온교환수지를 사용하는 이들물질의 분리 정제하는 방법, 이들 물질의 암을 포함한 면역관련질환의 예방, 치료, 및 치료후의 면역증강을 위한 약물로서의 이용에 관한 것, 이들물질의 면역학 기초연구를 위한 면역증강 시료로서의 이용에 관한 것이다.
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公开(公告)号：KR100302451B1
公开(公告)日：2001-12-28
申请号：KR1019980018158
申请日：1998-05-20
Applicant: 한국과학기술연구원
IPC: C12N9/26
Abstract: PURPOSE: Provided is a method for manufacturing invertase and yeast extract from yeast at the same time by extracting the invertase under optimal conditions to obtain the most active invertase, concentrating through membrane process to get the concentrated invertase solution and obtaining the yeast extract by self digestion method of the residue and the filtrate. CONSTITUTION: The method comprises the steps of: extracting the invertase from the shake culture medium of yeast with papain; centrifuging or micro-filtering the extract to separate the supernatant and the yeast; obtaining the invertase concentrate by ultrafiltering; and acquiring the yeast extract by self digesting the mixture of the above centrifuged or micro-filtered yeast and the above filtrate of the ultrafiltration.
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公开(公告)号：KR100320270B1
公开(公告)日：2002-01-10
申请号：KR1019990006137
申请日：1999-02-24
Applicant: 한국과학기술연구원
IPC: C12N9/20
Abstract: 본발명은유기용매로정제한리파제효소및 이를이용한키랄의약품의제조방법에관한것으로캔디다루고사유래의리파제효소를유기용매침전에의해부분정제하여광학분활반응에대해선택성이높은효소를제공하고상기광학분활반응성에대해선택성이높은효소는가수분해반응에의해광학적으로순수한비스토로이드계소염진통제 (S)-케토프로펜, (S)-이뷰프로펜, (S)-슈프로펜을생산하는뛰어난효과가있다.
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公开(公告)号：KR1019990085614A
公开(公告)日：1999-12-15
申请号：KR1019980018158
申请日：1998-05-20
Applicant: 한국과학기술연구원
IPC: C12N9/26
Abstract: 본 발명은 효모로부터 인버타제(invertase)와 효모엑기스를 동시에 제조하는 방법에 관한 것이다.
본 발명은 최대활성의 인버타제를 추출할 수 있는 최적 조건하에서 인버타제를 추출하고 막공정으로 농축하여 인버타제 농축액을 얻은 후 잔류 효모와 여과액을 혼합하고 자가소화반응시켜 효모엑기스를 얻으므로써 효모로부터 인버타제와 효모엑기스를 동시에 제조하는 뛰어난 효과가 있다.
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