公开(公告)号：KR1019980070038A

公开(公告)日：1998-10-26

申请号：KR1019970054925

申请日：1997-10-24

Applicant: 한국과학기술연구원

Inventor： 김철호 ,  김화영 ,  이상기 ,  최의성 ,  손정훈

IPC: C12N15/31 ,  C12N15/81

Abstract: 본 발명은 한세눌라 폴리모르파(Hansenula polymorpha) DL-1(ATCC 26012) 유래의 다중병렬도입형(multiple tandem integrating) 자기복제서열(autonomously replicating sequence, ARS) 및 이를 포함하는 발현벡터에 관한 것이다.

公开(公告)号：KR100252787B1

公开(公告)日：2000-04-15

申请号：KR1019970054925

申请日：1997-10-24

Applicant: 한국과학기술연구원

Inventor： 김철호 ,  김화영 ,  이상기 ,  최의성 ,  손정훈

IPC: C12N15/31 ,  C12N15/81

Abstract: PURPOSE: Provided are multiple tandem integrating autonomously replicating sequence(ARS) originated from Hansenula polymorpha and an expression vector including the same. The vector including this sequence is very useful for the production of an exogenous protein from Hansenula polymorpha due to its high efficiency and stability of transformation. CONSTITUTION: A vector for inserting polynucleotide coding hetero protein into chromosome of Hansenula polymorpha is composed of the followings: (a) multiple tandem integrating ARS including repetitive sequence of 5'-GGGTGGCG-3' at 3' terminal originated from Hansenula polymorpha DL-1(ATCC 26012); (b) a promotor; (c) polynucleotide coding hetero protein located at downstream of the promotor; and (d) a terminator located at downstream of the nucleotide.

Abstract translation: 目的：提供源自多形汉逊酵母的多重串联整合自主复制序列（ARS）和包含其的表达载体。 包含该序列的载体由于其转化的高效率和稳定性而非常适用于从多形汉逊酵母（Hansenula polymorpha）生产外源蛋白质。 构成：用于将多核苷酸编码杂蛋白插入多形汉逊酵母染色体的载体由以下组成：（a）多重串联整合ARS，包括来自多形汉逊酵母DL-1的3'末端的5'-GGGTGGCG-3'的重复序列 （ATCC 26012）; （b）促进者; （c）位于启动子下游的编码杂蛋白的多核苷酸; 和（d）位于核苷酸下游的终止子。