인체표피성장인자의유전자를갖는벡터및그를이용한인체표피성장인자의생산방법

    公开(公告)号:KR100295909B1

    公开(公告)日:2001-10-22

    申请号:KR1019940019637

    申请日:1994-08-09

    Abstract: PURPOSE: Provided are a vector including human epidermal growth factor(EGF) which promotes the growth of human epidermal cells and inhibits the secretion of gastric acid and a manufacturing method of EGF by using the vector. CONSTITUTION: The vector including EGF is manufactured by the steps of: inserting T-mox(MOX terminator) into a recognition site of pUC19 vector to prepare pUC-Tmox vector; inserting LEU2 gene into a recognition site of pUC-Tmox to prepare pUC-TaLE vector; inserting P-mox gene(MOX promotor), alpha pre-proleader(ppL) sequence and EGF gene into a recognition site of SalI of pUC-TaLE vector; cutting pU-EGF2 vector with EcoRI restriction enzyme; and inserting a fragment including P-mox/ppL/hEGF/T-mox into a recognition site of EcoRI of aP3 vector.

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