Abstract:
PURPOSE: Provided are a vector including human epidermal growth factor(EGF) which promotes the growth of human epidermal cells and inhibits the secretion of gastric acid and a manufacturing method of EGF by using the vector. CONSTITUTION: The vector including EGF is manufactured by the steps of: inserting T-mox(MOX terminator) into a recognition site of pUC19 vector to prepare pUC-Tmox vector; inserting LEU2 gene into a recognition site of pUC-Tmox to prepare pUC-TaLE vector; inserting P-mox gene(MOX promotor), alpha pre-proleader(ppL) sequence and EGF gene into a recognition site of SalI of pUC-TaLE vector; cutting pU-EGF2 vector with EcoRI restriction enzyme; and inserting a fragment including P-mox/ppL/hEGF/T-mox into a recognition site of EcoRI of aP3 vector.