公开(公告)号：KR1019980067138A

公开(公告)日：1998-10-15

申请号：KR1019970003022

申请日：1997-01-31

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  김용희 ,  배은희 ,  권익찬 ,  윤태영

IPC: A61K9/107

Abstract: 본 발명은 세포내로 유전자 DNA 및 생물학적 활성 약물을 전달할 수 있는 지방 유제 및 그것의 제조방법에 관한 것이다. 또한 본 발명은 지방 유제를 사용하여 세포내로 DNA와 같은 생물학적 활성 약물을 효과적으로 전달하는 방법에 관한 것이다. 본 발명의 지방 유제는 세포내로 유전자를 효과적으로 전달할 수 있고, 특히 혈청 중에서 높은 안정성 및 유전자 전달 효율을 나타냄으로써 임상에서 유전자 치료에 적합한 약물 전달 체계가 될 수 있다.

公开(公告)号：KR1020010063154A

公开(公告)日：2001-07-09

申请号：KR1019990060034

申请日：1999-12-21

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  배은희 ,  권익찬 ,  최귀원

IPC: C12N1/00

CPC classification number: C08J9/28 ,  C08B37/003 ,  C08J2201/0484 ,  C08J2305/08 ,  C08L5/08 ,  C12N5/0075 ,  C12N2533/72

Abstract: PURPOSE: Provided are multi-porous chitosan bead whose pores are big and relatively constant in size and a preparation method thereof. The bead is used as a matrix for cell cultivation and for the study of alternative metabolic organ, bone and cartilage and protein, antibiotics, anti-cancer medicine, polysaccharide, biological active material, animal hormone, and plant hormone. CONSTITUTION: A multi-porous chitosan bead has a pore being 5-20 micrometer in a size at its inside and outside. It is prepared by the following steps of: (a) preparing chitosan solution by dissolving chitosan in acetic acid aqueous solution, water-soluble chitosan solution by dissolving water-soluble chitosan in deionized solution or mixed solution thereof; (b) making it in bead form by adding organic solvent of low temperature or liquid nitrogen; and (c) freeze-drying the resultant bead.

Abstract translation: 目的：提供孔径大且尺寸相对恒定的多孔壳聚糖珠及其制备方法。 该珠被用作细胞培养的基质和用于替代代谢器官，骨和软骨和蛋白质，抗生素，抗癌药物，多糖，生物活性物质，动物激素和植物激素的研究。 构成：多孔壳聚糖珠的内部和外部具有5-20微米的孔径。 通过以下步骤制备：（a）通过将水溶性壳聚糖溶解在去离子溶液或其混合溶液中，通过将壳聚糖溶解在乙酸水溶液，水溶性壳聚糖溶液中制备壳聚糖溶液; （b）通过加入低温或液氮的有机溶剂使其呈珠粒形式; 和（c）冷冻干燥所得的珠粒。

公开(公告)号：KR100375422B1

公开(公告)日：2003-03-10

申请号：KR1019990060034

申请日：1999-12-21

Applicant: 한국과학기술연구원

Inventor： 정서영 ,  배은희 ,  권익찬 ,  최귀원

IPC: C12N1/00

CPC classification number: C08J9/28 ,  C08B37/003 ,  C08J2201/0484 ,  C08J2305/08 ,  C08L5/08 ,  C12N5/0075 ,  C12N2533/72

Abstract: The present invention relates to macroporous chitosan beads having 5-200 mum in size of relatively large and uniform pores that are distributed from surface to core region, and a preparation method thereof compring the following steps; by dropping chitosan solution, aqueous chitosan solution or their mixture into the low-temperature of organic solvent or liquid nitrogen; and by regulating pore size by phase separation method via temperature difference. The macroporous chitosan beads of the present invention make cell culture more efficient than the previous substrate, since cell can attach to them efficiently due to their large surface area, it is easy for cell to be injected into them and cell attached to them can exist longer due to their three-dimensional structure, therefore they can be used for a study about production of protein, abtibiotics, anticancer agent, polysaccharide, physiologically active agent, animal hormone, or plant hormone as well as a study about substitution of metabolic organs, cartilage or bone.

Abstract translation: 本发明涉及一种大孔径壳聚糖珠粒及其制备方法，所述大孔隙大小为5-200μm，具有较大且均匀的从表面到中心区域的孔隙，其制备方法包括以下步骤： 将壳聚糖溶液，壳聚糖水溶液或其混合物滴入低温有机溶剂或液氮中; 并通过温度差通过相分离法调节孔径。 本发明的大孔脱乙酰壳多糖珠粒使细胞培养比先前的基质更有效，因为细胞由于它们的大表面积而可以有效地附着到它们上，细胞很容易注入它们并且细胞附着在它们上的时间可以更长 由于它们的三维结构，因此它们可以用于研究蛋白质，抗生素，抗癌剂，多糖，生理活性剂，动物激素或植物激素的产生，以及关于代谢器官，软骨替代的研究 或骨头。