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公开(公告)号：KR100378323B1
公开(公告)日：2003-03-29
申请号：KR1019990014356
申请日：1999-04-22
IPC: C07H13/08
CPC classification number: A61K31/704 , A61K36/69
Abstract: The present invention relates to psychotropic polygalasaponins and its extraction and isolation from the roots of Polygalae tenuifolia. The inhibitory activity of the extract from the roots of Polygalae tenuifolia on stereotyped behaviors and climbing behaviors induced by apomorphine was examined, and a single extract fraction that showed an activity particularly in behavioral pharmacology model was obtained from the roots of Polygalae tenuifolia. The object of the invention is therefore aimed at providing a new version of antipsychotic drug, polygalasaponic compound itself and a method to extract and isolate polygalasaponic compound from the roots of Polygalae Tenuifolia.
Abstract translation: 本发明涉及精神性多果皂苷及其从远志（Polygalae tenuifolia）根提取和分离。 研究了远志根部提取物对阿朴吗啡诱导的刻板行为和爬行行为的抑制活性，并从远志草的根部获得了一种特别在行为药理学模型中表现出活性的单一提取物部分。 因此，本发明的目的是提供一种新的抗精神病药物，多聚半乳糖化合物本身以及从远志藤根提取和分离多聚半乳糖化合物的方法。
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公开(公告)号：KR100290532B1
公开(公告)日：2001-12-05
申请号：KR1019970040065
申请日：1997-08-22
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: PURPOSE: Provided are novel strain, fungus MT51005 (KCTC 8787P) and a composition for inhibiting the activity of phospholipase C which is an important enzyme for signal transfer in cell. The novel strain produces inhibitor of abnormal cell growth by inhibiting phospholipase C. CONSTITUTION: The composition for inhibiting the activity of phospholipase C comprises 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene-1'-nyl)-benzaldehyde represented by the formula(1) and/or 2,4-dihydroxy-3-ethyl-6-(1'-methylpentene)-benzaldehyde represented by the formula(2) separated and purified from fungus MT51005 (KCTC 8787P).
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公开(公告)号：KR1020000066933A
公开(公告)日：2000-11-15
申请号：KR1019990014356
申请日：1999-04-22
IPC: C07H13/08
CPC classification number: A61K31/704 , A61K36/69
Abstract: PURPOSE: A psychotropic polygalasaponin based compound is provided which inhibits a stereotyped behavior and climbing behavior which are induced by apomorphine. CONSTITUTION: A psychotropic polygalasaponin is represented by formula 1 representation. An extraction method are characterized by comprising the following steps: (i)after grinding Polygalae Radix up to 70-230 mesh, extracting three times with 3-5 fold amount of alcohol solution; (ii)evaporating and condensing the filtrate at 70-76 cm Hg and at 60-70°C; (iii)after suspending the condensate in distilled water of 10 fold volume ratio, shaking the above thing by adding alcohol solvent of 1-2 fold and extracting three times and separating alcohol layer; (iv)condensing the above alcohol solvent fraction at 70-76 cmHg and at 60-70°C and obtaining an extract; (v)after suspending the above extract with distilled water of 50-100 fold adsorbing in resin HP-20 and draining active material with 70% methanol and condensing for obtaining an extract; (vi)after condensing the above extract with distilled water of 50-100 fold 2-3 times in common ratio, suspending homogeneously with same amount of distilled water and freeze-drying for obtaining a powder extract; (vii)dissolving the powder extract in ether and obtaining a precipitate;(viii)after saturating the precipitate with distilled water, passing through HP-20 resin and eluting an active site with methanol and adsorbing in silica gel and eluting active material by using ethyl acetate/methanol/water; (ix)adsorbing the active material in silica gel again and fractionating with mixed solvent of trichloro methane/methanol/ethanol/10%-acetic acid.
Abstract translation: 目的：提供一种精神分析多聚赖草皂苷的化合物，其抑制由阿朴吗啡诱导的定型行为和攀爬行为。 构成：精神分裂多糖分子由式1表示。 提取方法的特征在于包括以下步骤：（i）研磨多达70-230目后，用3-5倍量的醇溶液萃取三次; （ii）在70-76cm Hg和60-70℃下蒸发并冷凝滤液; （iii）将冷凝物悬浮在10倍体积比的蒸馏水中后，加入1-2倍的酒精溶剂振荡上述物质，萃取3次，分离醇层; （iv）将上述醇溶剂级分在70-76cmHg和60-70℃下冷凝并得到提取物; （v）将吸附在树脂HP-20中的50-100倍的蒸馏水悬浮在上述提取物上，并用70％甲醇排出活性物质并冷凝得到提取物; （vi）将上述提取物与常规比例为50-100倍的蒸馏水冷凝2-3次后，用相同量的蒸馏水均匀悬浮，冷冻干燥得到粉末提取物; （vii）将粉末提取物溶解在乙醚中并获得沉淀物;（viii）在用蒸馏水使沉淀物饱和后，通过HP-20树脂并用甲醇洗脱活性位点并吸附在硅胶中，并用乙基 乙酸乙酯/甲醇/水; （ix）再次将活性物质吸附在硅胶中，并用三氯甲烷/甲醇/乙醇/ 10％乙酸的混合溶剂进行分馏。
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公开(公告)号：KR1020000026775A
公开(公告)日：2000-05-15
申请号：KR1019980044441
申请日：1998-10-19
Applicant: 한국과학기술연구원
IPC: A61K31/37
CPC classification number: C07D215/233 , A61K31/47
Abstract: PURPOSE: An angiotensin II receiving body binding inhibitor, which shows good inhibitive activity of the angiotensin II receiving body binding and good antagonistic action against vascular contraction induced by the angiotensin II, and a preparing method thereof are provided. CONSTITUTION: The angiotensin II receiving body binding inhibitor consists of a compound of following formula 1, where R represents aliphatic group having 5-20 carbon atoms, derivative thereof or salt thereof. The method comprises steps of: (i) extracting Evodiae Fructus with low class alcohol and then with acetate; and (ii) carrying out silica gel column chromatography, reverse phase low pressure column chromatography and high pressure liquid phase column chromatography on the extracted Evodiae Fructus. The pharmaceutical composition contains the compound of the formula 1 as an effective ingredient.
Abstract translation: 目的：提供血管紧张素II受体结合抑制剂，其具有良好的血管紧张素II受体结合的抑制活性和对血管紧张素II诱导的血管收缩的良好拮抗作用及其制备方法。 构成：血管紧张素II受体结合抑制剂由下式1的化合物组成，其中R表示具有5-20个碳原子的脂族基团，其衍生物或其盐。 该方法包括以下步骤：（i）用低级醇，然后用乙酸提取葫芦果; 和（ⅱ）对萃取的枸杞子进行硅胶柱层析，反相低压柱层析和高压液相柱层析。 药物组合物含有式1的化合物作为有效成分。
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5.发明公开신균주 곰팡이 MT51005(KCTC 8787P)와 이 균주로부터생산된 포스포리파제 C 활성저해용 조성물 失效一种新的菌株真菌MT51005（KCTC 8787P）和一种用于抑制菌株产生的磷脂酶C活性的组合物
公开(公告)号：KR1019990017226A
公开(公告)日：1999-03-15
申请号：KR1019970040065
申请日：1997-08-22
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: 본 발명은 신균주 곰팡이 MT51005(KCTC 8787P)와 포스포리파제 C 활성저해용 조성물에 관한 것으로서, 더욱 상세하게는 세포내 신호전달과정의 중요효소인 포스포리파제 C(Phospholipase C)의 활성을 저해하여 세포의 이상증식을 억제할 수 있는 물질을 생산하는 신규 곰팡이 균주 MT51005와, 곰팡이 MT51005의 배양액으로부터 분리 정제된 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐-1'-닐)-벤즈알데하이드, 2,4-디하이드록시-3-에틸-6-(1'-메틸펜텐)-벤즈알데하이드, 또는 이들 각각을 유효성분으로 함유한 포스포리파제 C 활성저해용 조성물에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR100314903B1
公开(公告)日：2001-11-23
申请号：KR1019990005821
申请日：1999-02-22
Applicant: 한국과학기술연구원
IPC: C12N1/16
Abstract: 본발명은핵산을다량함유하고있는칸디다속의새로운효모균주인칸디다노베지카 ()에관한것으로, 구체적으로살펴보면본 발명의칸디다속의새로운미생물인칸디다노베지카 CRM-9802 (CRM-9802)는조미성분인 GMP(guanosine monophosphate)와 IMP(inosine monophosphate)로변환시킬수 있는 RNA의함량이높은유용한특징을가지고있으므로, RNA 분해법에의한핵산조미료생산에있어우수한 RNA제공원으로이용될수 있을뿐 아니라효모자체의균체추출물상태로만들어지는천연조미료의제조에효과적으로사용될수 있다.
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7.发明授权신균주 스트렙토마이세스속 MT1882-V(KCTC 8800P)와 피에리시딘 A1의 인터루킨-2생성 억제제로서의 용도 失效新的STREPTOMYCES SP。 MT1882-V和PIERICIDIN A1作为免疫抑制剂
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8.发明授权신균주 곰팡이 슈달레세리아 속 MT60109(KCTC 8804P)와 포스포리파제 C 활성저해용 조성물 失效PSEUDALLESCHERIA SP。 MT60109和PHOSPHOLIPASE C抑制剂的药物组合物
公开(公告)号：KR100224476B1
公开(公告)日：1999-10-15
申请号：KR1019970052304
申请日：1997-10-13
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: 본 발명은 신균주 곰팡이 슈탈레세리아 속(Pseudallescheria sp.) MT60109(KCTC 8804P)와 포스포리파제 C 활성저해용 조성물에 관한 것으로, 더욱 상세하게는 세포내 신호전달과정이 중요효소인 포스포리파제(Phospholipase C)의 활성을 저해하여 세포의 이상신호 전달과정을 억제할 수 있는 물질을 생산하는 신규 곰팡이 균주 슈달레세리아 속 MT60109와, 이 균주의 배양액으로부터 분리 정제된 신규 화합물 티엘라빈 F(thielavin F)를 유효성분으로 함유한 포스포리파제 C 활성저해용 조성물에 관한 것이다.
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9.发明公开신균주 곰팡이 슈달레세리아 속 MT60109(KCTC 8804P)와 포스포리파제 C 활성저해용 조성물 失效抑制磷脂酶C活性的组合物和新的菌株真菌Sduarecia属MT60109（KCTC 8804P）
公开(公告)号：KR1019990031527A
公开(公告)日：1999-05-06
申请号：KR1019970052304
申请日：1997-10-13
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: 본 발명은 신균주 곰팡이 슈달레세리아 속(
Pseudallescheria sp.) MT60109(KCTC 8804P)와 포스포리파제 C 활성저해용 조성물에 관한 것으로서, 더욱 상세하게는 세포내 신호전달과정의 중요효소인 포스포리파제 C(Phospholipase C)의 활성을 저해하여 세포의 이상신호 전달과정을 억제할 수 있는 물질을 생산하는 신규 곰팡이 균주 슈달레세리아 속 MT60109와, 이 균주의 배양액으로부터 분리 정제된 신규 화합물 티엘라빈 F(thielavin F)를 유효성분으로 함유한 포스포리파제 C 활성저해용 조성물에 관한 것이다.-
公开(公告)号：KR1020000056471A
公开(公告)日：2000-09-15
申请号：KR1019990005821
申请日：1999-02-22
Applicant: 한국과학기술연구원
IPC: C12N1/16
Abstract: PURPOSE: New strain of Candidanorvegica, CRM-9802 (KCTC 8900P) containing high concentration of ribonucleic acid (RNA) is provided which is used to manufacture a nucleotide spice from RNA of Candida cell extract. CONSTITUTION: Soil sample is mixed with sterile saline and vortexed. The supernatant is plated on YM agar and incubated at 30°C for 24-48 hours. The isolated colony is cultured and RNA content of each colony is measured. The colony showing high content of RNA is selected and its morphological, physiological and cultural characteristics are identified. The isolated yeast forms creamy egg shape colony on YM plate and does not make spores. The range of temperature for growth is from 15 to 35°C. Under anaerobic condition it metabolizes D-glucose and D-cellobiose as carbon sources. On the other hand, D-xylose, L-rhamnose, cellobios, salicin, arbutin, glycerol, D-glucitol, citrate and ethanol can be metabolized at aerobic conditions. Nitrate, nitrite, L-lysine and creatine can be used as a nitrogen source at aerobic conditions. The yeast is cultured in YM medium and harvested at 10 OD and RNA content of cell is measured by orcinol method. The RNA content of Candida norvegica is 12-13% of cell mass.
Abstract translation: 目的：提供含有高浓度核糖核酸（RNA）的新一代Candidanorvegica，CRM-9802（KCTC 8900P），用于从念珠菌细胞提取物的RNA制备核苷酸调味剂。 构成：将土壤样品与无菌盐水混合并涡旋。 将上清液接种在YM琼脂上，并在30℃下孵育24-48小时。 培养分离的菌落，测定每个菌落的RNA含量。 选择显示高含量RNA的菌落，确定其形态，生理和文化特征。 分离的酵母在YM板上形成奶油蛋形菌落，不产生孢子。 生长温度范围为15〜35℃。 在厌氧条件下，将D-葡萄糖和D-纤维二糖代谢为碳源。 另一方面，D-木糖，L-鼠李糖，纤维二糖，水杨蛋白，熊果苷，甘油，D-葡萄糖醇，柠檬酸盐和乙醇可以在需氧条件下代谢。 硝酸盐，亚硝酸盐，L-赖氨酸和肌酸可用作有氧条件下的氮源。 将酵母在YM培养基中培养并在10℃下收获，并通过α-淀粉酶法测定细胞的RNA含量。 假丝酵母的RNA含量为细胞质量的12-13％。
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