公开(公告)号：KR1019940026066A

公开(公告)日：1994-12-08

申请号：KR1019940010902

申请日：1994-05-19

Applicant: 한국과학기술연구원 ,  주식회사 녹십자홀딩스

Inventor： 유명희 ,  권기선 ,  이기녕 ,  신화수

IPC: C07K14/655

公开(公告)号：KR100133252B1

公开(公告)日：1998-04-14

申请号：KR1019940010902

申请日：1994-05-19

Applicant: 한국과학기술연구원 ,  주식회사 녹십자홀딩스

Inventor： 유명희 ,  권기선 ,  이기녕 ,  신화수

IPC: C07K14/655

Abstract: The mutagenized recombinant AT of the present invention wherein some amino acids of the amino acid sequence of a wild-type AT are replaced with another amino acid, which has an enhanced thermoresistance compared to the wild-type AT while maintaining its activity. A vector containing a gene encoding the recombinant AT, a microorganism transformed with the vector and a process for producing the recombinant AT with a higher thermoresistance using the microorganism are also disclosed.

Abstract translation: 本发明的诱变重组AT，其中野生型AT的氨基酸序列的一些氨基酸被替换为另一种氨基酸，其与野生型AT相比具有增强的耐热性，同时保持其活性。 还公开了含有编码重组AT的基因的载体，用载体转化的微生物和使用该微生物生产具有较高的热电阻的重组AT的方法。

公开(公告)号：KR1019930000686A

公开(公告)日：1993-01-15

申请号：KR1019910010937

申请日：1991-06-28

Applicant: 한국과학기술연구원

Inventor： 유명희 ,  이승철 ,  신화수 ,  이기녕 ,  이상철

IPC: C12N15/57

Abstract: 내용 없음

公开(公告)号：KR100072006B1

公开(公告)日：1994-03-17

申请号：KR1019910010937

申请日：1991-06-28

Applicant: 한국과학기술연구원

Inventor： 유명희 ,  이승철 ,  신화수 ,  이기녕 ,  이상철

IPC: C12N15/57

公开(公告)号：KR1019930012114B1

公开(公告)日：1993-12-24

申请号：KR1019910010937

申请日：1991-06-28

Applicant: 한국과학기술연구원

Inventor： 유명희 ,  이승철 ,  신화수 ,  이기녕 ,  이상철

IPC: C12N15/57

Abstract: A genomic gene encoding of human alpha-1-antitrypsin was cloned into a plasmid pET8C. E.coli BL21 (DE3) was transformed by the recombinant plasmids and a transformant (KCTC 0009BP) carrying the recombinant plasmid pEAT8 was screened. The transformant was cultured in a M9ZB medium at 37 deg.C and IPTG (0.04 mM) was added to the culture at OD600 = 0.7. After incubation at 40 deg.C for 3 hrs, the cell pellet was recovered by centrifugation. The cell pellet was treated by sonication and the precipitate containing alpha-1- antitrypsin was recovered by centrifugation. The precipitate was dissolved in a buffer containing 8M area and the alpha-1-antitrypsin was purified by DEAE-Sephacel chromatography and Mono-Q chromatography of FPLC.

Abstract translation: 将编码人α-1-抗胰蛋白酶的基因组基因克隆到质粒pET8C中。 通过重组质粒转化大肠杆菌BL21（DE3），筛选携带重组质粒pEAT8的转化体（KCTC 0009BP）。 将转化体在37℃的M9ZB培养基中培养，并将ODT = 0.7的IPTG（0.04mM）加入培养物中。 在40℃温育3小时后，通过离心回收细胞沉淀。 通过超声处理细胞沉淀，并通过离心回收含有α-1-抗胰蛋白酶的沉淀物。 将沉淀物溶解在含有8M面积的缓冲液中，通过DEAE- Sephacel层析和FPLC的Mono-Q色谱纯化α-1-抗胰蛋白酶。