公开(公告)号：KR1019940000578A

公开(公告)日：1994-01-18

申请号：KR1019920011007

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  최의성 ,  손정훈 ,  이상권

IPC: C12N15/15

公开(公告)号：KR100102176B1

公开(公告)日：1996-07-16

申请号：KR1019920011007

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  최의성 ,  손정훈 ,  이상권

IPC: C12N15/15

公开(公告)号：KR1019960001818B1

公开(公告)日：1996-02-05

申请号：KR1019920011007

申请日：1992-06-24

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  최의성 ,  손정훈 ,  이상권

IPC: C12N15/15

Abstract: The hirudin expression vector pYEGa-HIR5 (KCTC 8518P) containing hirudin gene (I) consists of linked promoter GAL1-GAL10, alpha factor of yeasts (a on plasmid means alpha), peptide sequence for leu-asp-lys-arg which is hydrolyzed by KEX2 as operator endopeptidase and hirudin gene. The other plasmid pYEGa-HIR525 (KCTC 8519BP) (II) consists of GAL10 as promoter rather than GAL1-GAL10 of (I). (I) and (II) are useful for yeast expression to solve the problems which in E. coli the expressed hirudin is hydrolysed and the N-terminal methionine modified hirudin does not have the activity.

Abstract translation: 含有水蛭素基因（I）的水蛭素表达载体pYEGa-HIR5（KCTC 8518P）由连接的启动子GAL1-GAL10，酵母的α因子（a在质粒表示α）组成，leu-asp-lys-arg的肽序列被水解 通过KEX2作为操作者内肽酶和水蛭素基因。 另一个质粒pYEGa-HIR525（KCTC 8519BP）（II）由GAL10作为启动子而不是（I）的GAL1-GAL10组成。 （I）和（II）可用于酵母表达以解决在大肠杆菌中表达的水蛭素被水解并且N端甲硫氨酸修饰的水蛭素不具有活性的问题。