公开(公告)号：KR1020030038057A

公开(公告)日：2003-05-16

申请号：KR1020010069469

申请日：2001-11-08

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  이정란 ,  김영옥 ,  홍지연

IPC: G01N33/533

Abstract: PURPOSE: A single-stepped immunocomplex detection reagent is provided which has high reproducibility and increased stability. It is used for the primary screening to detect agricultural chemicals. CONSTITUTION: A single-stepped immunocomplex detection reagent comprises an immune antibody for a compound represented by the formula(1) and a fluorescent tracer antigen. In the formula(1), R1 is the alkyl group, an ethyl group (-CH2CH3) or an isopropyl group (-CH(CH3)2); R2 is the alkyl group, an ethyl group (-CH2CH3), an isopropyl group (-CH(CH3)2) or a cyanoisopropyl group (-C(CN)-(CH3)2); and R3 is chloride or methyl sulfide (-SCH3). The immune antibody is selected from the group consisting of antibodies for atrazine, simazine, cyanazine, and ametryn. The fluorescent tracer antigen is prepared by labeling R2 and/or R3 of the compound of the formula(1) with a fluorescent material.

Abstract translation: 目的：提供具有高再现性和稳定性的单步免疫复合物检测试剂。 它用于初步筛选以检测农药。 构成：单步免疫复合物检测试剂包含由式（1）表示的化合物的免疫抗体和荧光示踪剂抗原。 式（1）中，R 1为烷基，乙基（-CH 2 CH 3）或异丙基（-CH（CH 3）2））。 R2是烷基，乙基（-CH2CH3），异丙基（-CH（CH3）2）或氰基异丙基（-C（CN） - （CH3）2））。 且R 3为氯或甲硫醚（-SCH 3）。 免疫抗体选自莠去津，西马拉平，氰嗪和莠去津的抗体。 通过用荧光材料标记式（1）化合物的R 2和/或R 3来制备荧光示踪剂抗原。

公开(公告)号：KR1020030008748A

公开(公告)日：2003-01-29

申请号：KR1020010043630

申请日：2001-07-20

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  김은경 ,  최정은 ,  이정란

IPC: G01N33/543

Abstract: PURPOSE: Provided are an mmunostrip paper for the simultaneous detection of philopon and cannabis without involving additional reagents. CONSTITUTION: The mmunostrip paper for the simultaneous detection of philopon and cannabis comprises the parts of: (i) a sample membrane(1) coated with protein and sugar; (ii) a glass fiber membrane(2) positioned under the sample membrane(1) and containing a drug antibody-colored minute particle complex; (iii) a polyester supporting nitrocellulose membrane(3) connected vertically to the glass fiber membrane(2), and containing a test resulting line as a drug-protein complex and a test controlling line as a second antibodies of the drugs in different sites; (iv) a reaction solution absorbing membrane(4) connected vertically to the polyester supporting nitrocellulose membrane(3); and supporting bars positioned under each membrane, wherein the sugar is dextran; the protein is bovine serum albumin; the colored minute particle is colloidal; and the supporting bar is made of polyester resin.

Abstract translation: 目的：提供用于同时检测philopon和大麻而不涉及额外试剂的mmunostrip纸。 构成：用于同时检测philopon和大麻的mmunostrip纸包括以下部分：（i）涂有蛋白质和糖的样品膜（1）; （ii）位于样品膜（1）下方并含有药物抗体着色的微粒子复合体的玻璃纤维膜（2） （iii）垂直于玻璃纤维膜（2）连接的聚酯支撑硝酸纤维素膜（3），并且包含作为药物 - 蛋​​白质复合物的测试结果线和作为不同部位药物的第二抗体的测试控制线; （iv）垂直于聚酯支撑硝酸纤维素膜（3）连接的反应溶液吸收膜（4）; 和位于每个膜下方的支撑杆，其中所述糖是葡聚糖; 蛋白质是牛血清白蛋白; 有色微粒是胶体的; 支撑杆由聚酯树脂制成。

公开(公告)号：KR100444698B1

公开(公告)日：2004-08-21

申请号：KR1020010069469

申请日：2001-11-08

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  이정란 ,  김영옥 ,  홍지연

IPC: G01N33/533

Abstract: PURPOSE: A single-stepped immunocomplex detection reagent is provided which has high reproducibility and increased stability. It is used for the primary screening to detect agricultural chemicals. CONSTITUTION: A single-stepped immunocomplex detection reagent comprises an immune antibody for a compound represented by the formula(1) and a fluorescent tracer antigen. In the formula(1), R1 is the alkyl group, an ethyl group (-CH2CH3) or an isopropyl group (-CH(CH3)2); R2 is the alkyl group, an ethyl group (-CH2CH3), an isopropyl group (-CH(CH3)2) or a cyanoisopropyl group (-C(CN)-(CH3)2); and R3 is chloride or methyl sulfide (-SCH3). The immune antibody is selected from the group consisting of antibodies for atrazine, simazine, cyanazine, and ametryn. The fluorescent tracer antigen is prepared by labeling R2 and/or R3 of the compound of the formula(1) with a fluorescent material.

Abstract translation: 目的：提供单步免疫复合物检测试剂，具有高重复性和稳定性。 它用于初级筛选以检测农药。 组成：单步免疫复合物检测试剂包含式（1）代表的化合物和荧光示踪抗原的免疫抗体。 式（1）中，R 1为烷基，乙基（-CH 2 CH 3）或异丙基（-CH（CH 3）2）。 R 2为烷基，乙基（-CH 2 CH 3），异丙基（-CH（CH 3）2）或氰基异丙基（-C（CN） - （CH 3）2）。 和R3是氯或甲硫（-SCH3）。 免疫抗体选自阿特拉津，西玛津，氰嗪和莠灭净的抗体。 荧光示踪抗原通过用荧光物质标记式（1）化合物的R2和/或R3来制备。