公开(公告)号：KR1020020082660A

公开(公告)日：2002-10-31

申请号：KR1020010022373

申请日：2001-04-25

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  홍지연

IPC: C12N5/18

CPC classification number: C12N5/163 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/31 ,  C12N2510/02 ,  G01N33/5308 ,  G01N33/577

Abstract: PURPOSE: Provided are hybridoma cell lines producing monoclonal antibodies simultaneously responsive to DDT, and its metabolites, thereby detecting DDT and its metabolites remaining in the body or the environment. CONSTITUTION: The hybridoma cell lines, DDA1A3(KCLRF-BP-00040), DDA1A1(KCLRF-BP-00039) and DDA1A4(KCLRF-BP-00041), produce monoclonal antibodies which simultaneously react to DDT and its metabolites, such as dichlorodiphenyltrichloroethan(DDT), dichlorodiphenyldichloroethylene(DDE), dichlorodiphenyldichloroethan(DDD) and dichlorodiphenylacetic acid(DDA). Therefore, DDT and its metabolites can be detected by using the monoclonal antibodies produced by the above hybridoma cell lines and their matching ligands coated on a microplate.

Abstract translation: 目的：提供产生同时对滴滴涕反应的单克隆抗体及其代谢物的杂交瘤细胞系，从而检测残留在人体或环境中的滴滴涕及其代谢物。 构成：杂交瘤细胞系DDA1A3（KCLRF-BP-00040），DDA1A1（KCLRF-BP-00039）和DDA1A4（KCLRF-BP-00041）产生与DDT及其代谢物（如二氯二苯基三氯乙烷 DDT），二氯二苯基二氯乙烯（DDE），二氯二苯基二氯乙烷（DDD）和二氯二苯乙酸（DDA）。 因此，可以通过使用由上述杂交瘤细胞系产生的单克隆抗体及其匹配的涂布在微量培养板上的配体来检测DDT及其代谢物。

公开(公告)号：KR100444698B1

公开(公告)日：2004-08-21

申请号：KR1020010069469

申请日：2001-11-08

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  이정란 ,  김영옥 ,  홍지연

IPC: G01N33/533

Abstract: PURPOSE: A single-stepped immunocomplex detection reagent is provided which has high reproducibility and increased stability. It is used for the primary screening to detect agricultural chemicals. CONSTITUTION: A single-stepped immunocomplex detection reagent comprises an immune antibody for a compound represented by the formula(1) and a fluorescent tracer antigen. In the formula(1), R1 is the alkyl group, an ethyl group (-CH2CH3) or an isopropyl group (-CH(CH3)2); R2 is the alkyl group, an ethyl group (-CH2CH3), an isopropyl group (-CH(CH3)2) or a cyanoisopropyl group (-C(CN)-(CH3)2); and R3 is chloride or methyl sulfide (-SCH3). The immune antibody is selected from the group consisting of antibodies for atrazine, simazine, cyanazine, and ametryn. The fluorescent tracer antigen is prepared by labeling R2 and/or R3 of the compound of the formula(1) with a fluorescent material.

Abstract translation: 目的：提供单步免疫复合物检测试剂，具有高重复性和稳定性。 它用于初级筛选以检测农药。 组成：单步免疫复合物检测试剂包含式（1）代表的化合物和荧光示踪抗原的免疫抗体。 式（1）中，R 1为烷基，乙基（-CH 2 CH 3）或异丙基（-CH（CH 3）2）。 R 2为烷基，乙基（-CH 2 CH 3），异丙基（-CH（CH 3）2）或氰基异丙基（-C（CN） - （CH 3）2）。 和R3是氯或甲硫（-SCH3）。 免疫抗体选自阿特拉津，西玛津，氰嗪和莠灭净的抗体。 荧光示踪抗原通过用荧光物质标记式（1）化合物的R2和/或R3来制备。

公开(公告)号：KR100428812B1

公开(公告)日：2004-04-28

申请号：KR1020010022373

申请日：2001-04-25

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  홍지연

IPC: C12N5/18

Abstract: PURPOSE: Provided are hybridoma cell lines producing monoclonal antibodies simultaneously responsive to DDT, and its metabolites, thereby detecting DDT and its metabolites remaining in the body or the environment. CONSTITUTION: The hybridoma cell lines, DDA1A3(KCLRF-BP-00040), DDA1A1(KCLRF-BP-00039) and DDA1A4(KCLRF-BP-00041), produce monoclonal antibodies which simultaneously react to DDT and its metabolites, such as dichlorodiphenyltrichloroethan(DDT), dichlorodiphenyldichloroethylene(DDE), dichlorodiphenyldichloroethan(DDD) and dichlorodiphenylacetic acid(DDA). Therefore, DDT and its metabolites can be detected by using the monoclonal antibodies produced by the above hybridoma cell lines and their matching ligands coated on a microplate.

Abstract translation: 目的：提供产生单克隆抗体的杂交瘤细胞系，其同时对DDT及其代谢物有反应，由此检测残留在体内或环境中的DDT及其代谢物。 组成：杂交瘤细胞系DDA1A3（KCLRF-BP-00040），DDA1A1（KCLRF-BP-00039）和DDA1A4（KCLRF-BP-00041）产生可同时与DDT及其代谢物反应的单克隆抗体，如二氯二苯基三氯乙烷 DDT），二氯二苯基二氯乙烯（DDE），二氯二苯基二氯乙烷（DDD）和二氯二苯基乙酸（DDA）。 因此，通过使用由上述杂交瘤细胞系产生的单克隆抗体及其在微孔板上包被的匹配配体，可以检测到DDT及其代谢物。

公开(公告)号：KR1020030038057A

公开(公告)日：2003-05-16

申请号：KR1020010069469

申请日：2001-11-08

Applicant: 한국과학기술연구원

Inventor： 최명자 ,  이정란 ,  김영옥 ,  홍지연

IPC: G01N33/533

Abstract: PURPOSE: A single-stepped immunocomplex detection reagent is provided which has high reproducibility and increased stability. It is used for the primary screening to detect agricultural chemicals. CONSTITUTION: A single-stepped immunocomplex detection reagent comprises an immune antibody for a compound represented by the formula(1) and a fluorescent tracer antigen. In the formula(1), R1 is the alkyl group, an ethyl group (-CH2CH3) or an isopropyl group (-CH(CH3)2); R2 is the alkyl group, an ethyl group (-CH2CH3), an isopropyl group (-CH(CH3)2) or a cyanoisopropyl group (-C(CN)-(CH3)2); and R3 is chloride or methyl sulfide (-SCH3). The immune antibody is selected from the group consisting of antibodies for atrazine, simazine, cyanazine, and ametryn. The fluorescent tracer antigen is prepared by labeling R2 and/or R3 of the compound of the formula(1) with a fluorescent material.

Abstract translation: 目的：提供具有高再现性和稳定性的单步免疫复合物检测试剂。 它用于初步筛选以检测农药。 构成：单步免疫复合物检测试剂包含由式（1）表示的化合物的免疫抗体和荧光示踪剂抗原。 式（1）中，R 1为烷基，乙基（-CH 2 CH 3）或异丙基（-CH（CH 3）2））。 R2是烷基，乙基（-CH2CH3），异丙基（-CH（CH3）2）或氰基异丙基（-C（CN） - （CH3）2））。 且R 3为氯或甲硫醚（-SCH 3）。 免疫抗体选自莠去津，西马拉平，氰嗪和莠去津的抗体。 通过用荧光材料标记式（1）化合物的R 2和/或R 3来制备荧光示踪剂抗原。