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1.发明授权이.콜라이 세포로부터 유용한 펩티드를 유전공학적 방법으로 생산하기 위해 제조된 발현벡터, 형질전환균주 및 그의 제조방법 失效通过基因工程方法，转化菌株及其生产方法从大肠杆菌细胞制备有用肽的表达载体及其制备方法
公开(公告)号：KR1019890005072B1
公开(公告)日：1989-12-09
申请号：KR1019870014289
申请日：1987-12-15
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: In the method, PCT10 is treated with BamHI in buffer II to give 1.1 kb DNA segment containing β-galactosidase sequence. The segment is treated with Sau3A to get 600 bp DNA segment to code N-terminal 200 amino acids of β-galactosidase. The segment is ligated with PUC18 to give PUCCT30 (KCTC 83249). PUCCT 30 is treated with BamHI and SalI to give 600 bp DNA segment, which is ligated with pT-6IN to get PCT 30.
Abstract translation: 在该方法中，PCT10在缓冲液II中用BamHI处理，得到含有β-半乳糖苷酶序列的1.1kb DNA片段。 该段用Sau3A处理得到600bp的DNA片段，以编码β-半乳糖苷酶的N-末端200个氨基酸。 将该片段与PUC18连接，得到PUCCT30（KCTC 83249）。 用BamHI和SalI处理PUCCT 30，得到600bp的DNA片段，其与pT-6IN连接得到PCT 30。
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