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公开(公告)号：KR101612906B1
公开(公告)日：2016-04-19
申请号：KR1020090067159
申请日：2009-07-23
Abstract: 본발명은피부의기능을향상시키는역학자극의스크리닝(screening) 방법으로서, (a) 피부에역학자극을반복하여가하는단계; (b) 상기역학자극에의한피부의생화학적변화를측정하는단계; 및 (c) 상기생화학적변화를기반으로피부에유용한역학자극을선정하는단계를포함한다. 본발명에따른스크리닝방법은외부자극에대한피부의생화학적변화를측정하여분석함으로써, 피부세포의기능을향상시키는생화학적변화를유도하는역학자극의종류와형태, 크기, 세기, 주기및 시간등을효율적으로스크리닝할수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020140121554A
公开(公告)日：2014-10-16
申请号：KR1020130037728
申请日：2013-04-05
Abstract: 본 발명은 멜라닌 합성 제어 인자 스크리닝 방법 및 장치, 미백 물질 스크리닝 방법에 관한 것으로서, 본 발명의 일 실시예에 따른 멜라닌 합성 제어 인자를 스크리닝 방법은 멜라닌 합성 사이클을 구성하는 단백질들을 각각의 노드로 하여 네트워크화한 하나 이상의 멜라닌 합성 네트워크에 세포 신호전달 경로 신호, 다른 네트워크에 의한 출력 신호, 자외선 신호 및 멜라닌 신호 중 하나 이상을 입력하고, 상기 멜라닌 합성 사이클을 구성하는 단백질들 중 하나 이상의 활성 정보를 변경하는 신호를 해당 노드에 입력하여 생성된 출력 신호를 이용하여 멜라닌 합성 제어 인자를 스크리닝한다.
Abstract translation: 本发明涉及一种用于筛选黑色素合成控制因子的方法和装置，以及用于筛选美白物质的方法。 根据本发明的一个实施方案的筛选黑色素合成控制因子的方法使用通过在细胞信号转导途径信号，输出信号，紫外线信号中输入一个或多个信号而产生的输出信号来屏蔽黑色素合成控制因子 ，以及使用构成黑色素合成循环的蛋白质作为每个网络的节点联网的一种或多种黑色素合成网络的黑色素信号，以及将构成黑色素合成循环的蛋白质的至少一种激活信息修改为相应的信号 节点。
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公开(公告)号：KR100451062B1
公开(公告)日：2004-10-02
申请号：KR1020020025356
申请日：2002-05-08
Applicant: 한국과학기술원
IPC: C12Q1/68
Abstract: PURPOSE: A detection method of cyanobacteria producing microcystin by using PCR is provided, thereby detecting a small amount of microcystin-producing Cyanobacteria. Therefore, water quality can be appropriately maintained. CONSTITUTION: A primer capable of amplifying a specific region of a peptide synthetase gene that is specifically present in the microcystin producing Cyanobacteria is provided, wherein the specific region of the peptide synthetase gene is adenylation domain; the primer is a pair of primers consisting of one primer selected from MCF-1 having the nucleotide sequence of SEQ ID NO: 1 or MCF-2 having the nucleotide sequence of SEQ ID NO: 2, and another primer selected from MCR-1 having the nucleotide sequence of SEQ ID NO: 3 or MCR-2 having the nucleotide sequence of SEQ ID NO: 4. A detection method of microcystin-producing Cyanobacteria by using PCR comprises the steps of: (1) extracting DNAs from various microorganisms; (2) PCR amplifying the DNAs as template using a primer capable of amplifying a specific region of the peptide synthetase gene; (3) subjecting the amplified genes to electrophoresis; and (4) dyeing of the developed gene to analyze the PCR product.
Abstract translation: 目的：提供一种通过使用PCR产生产生微囊藻毒素的蓝细菌的检测方法，由此检测少量产生微囊藻毒素的蓝细菌。 因此，可以适当地维持水质。 本发明提供了能够扩增产生微藻素的蓝细菌中特异性存在的肽合成酶基因的特定区域的引物，其中肽合成酶基因的特定区域是腺苷酸化结构域; 所述引物是由选自具有SEQ ID NO：1的核苷酸序列的MCF-1或具有SEQ ID NO：2的核苷酸序列的MCF-2的一种引物组成的一对引物，和选自MCR-1的另一种引物，所述引物具有 具有SEQ ID NO：4的核苷酸序列的SEQ ID NO：3或MCR-2的核苷酸序列。通过使用PCR检测产生微囊藻毒素的蓝细菌的方法包括以下步骤：（1）从各种微生物中提取DNA; （2）使用能够扩增肽合成酶基因的特定区域的引物，将该DNA作为模板进行PCR扩增; （3）将扩增的基因进行电泳; 和（4）对开发的基因进行染色以分析PCR产物。
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公开(公告)号：KR1020030087336A
公开(公告)日：2003-11-14
申请号：KR1020020025356
申请日：2002-05-08
Applicant: 한국과학기술원
IPC: C12Q1/68
Abstract: PURPOSE: A detection method of cyanobacteria producing microcystin by using PCR is provided, thereby detecting a small amount of microcystin-producing Cyanobacteria. Therefore, water quality can be appropriately maintained. CONSTITUTION: A primer capable of amplifying a specific region of a peptide synthetase gene that is specifically present in the microcystin producing Cyanobacteria is provided, wherein the specific region of the peptide synthetase gene is adenylation domain; the primer is a pair of primers consisting of one primer selected from MCF-1 having the nucleotide sequence of SEQ ID NO: 1 or MCF-2 having the nucleotide sequence of SEQ ID NO: 2, and another primer selected from MCR-1 having the nucleotide sequence of SEQ ID NO: 3 or MCR-2 having the nucleotide sequence of SEQ ID NO: 4. A detection method of microcystin-producing Cyanobacteria by using PCR comprises the steps of: (1) extracting DNAs from various microorganisms; (2) PCR amplifying the DNAs as template using a primer capable of amplifying a specific region of the peptide synthetase gene; (3) subjecting the amplified genes to electrophoresis; and (4) dyeing of the developed gene to analyze the PCR product.
Abstract translation: 目的：提供使用PCR产生微藻素的蓝细菌的检测方法，从而检测少量产生微囊藻毒素的蓝藻细菌。 因此，可以适当地保持水质。 提供了能够扩增特异性存在于产生微囊藻毒素的蓝细菌的肽合成酶基因的特定区域的引物，其中肽合成酶基因的特定区域是腺苷酸化结构域; 引物是由选自具有SEQ ID NO：1的核苷酸序列的MCF-1或具有SEQ ID NO：2的核苷酸序列的MCF-2的一种引物组成的一对引物，以及选自MCR-1的另一种引物，其具有 具有SEQ ID NO：4的核苷酸序列的SEQ ID NO：3或MCR-2的核苷酸序列。通过使用PCR的微囊藻毒素生产蓝细菌的检测方法包括以下步骤：（1）从各种微生物中提取DNA; （2）使用能够扩增肽合成酶基因的特定区域的引物，PCR扩增DNA作为模板; （3）对经扩增的基因进行电泳; 和（4）开发基因的染色以分析PCR产物。
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公开(公告)号：KR1020100010916A
公开(公告)日：2010-02-02
申请号：KR1020090067159
申请日：2009-07-23
CPC classification number: C12Q1/6883 , C12Q2600/158
Abstract: PURPOSE: A method for screening dynamic stimulation for improving skin function is provided to measure biochemical change against external stimulation and screen kind, form, size, intensity, and time of dynamic stimulation. CONSTITUTION: A method for screening dynamic stimulation for improving skin function comprises: a first step of performing repeatitive dynamic stimulation to skin; a second step of measuring biochemical change of skin by dynamic stimulation; and a third step of selecting useful dynamic stimulation based on biochemical change. The measurement is performed by measuring mRNA expression level of fibroblast-derived gene. In the third step, nerve growth factor, neurotrophin 3, TIMP 1, TrkA, TrkC, TGF alpha, TGF beta1, TGF beta2 or TGF beta3 gene is selected as dynamic stimulation.
Abstract translation: 目的：提供一种用于筛选动态刺激以改善皮肤功能的方法，以测量对外部刺激和屏幕种类，形态，大小，强度和动态刺激时间的生物化学变化。 构成：用于筛选动态刺激以改善皮肤功能的方法包括：对皮肤进行重复动态刺激的第一步骤; 通过动态刺激测量皮肤生化变化的第二步; 以及基于生物化学变化选择有用的动态刺激的第三步。 通过测量成纤维细胞衍生基因的mRNA表达水平进行测量。 在第三步中，选择神经生长因子，神经营养因子3，TIMP1，TrkA，TrkC，TGFα，TGFβ1，TGFβ2或TGFβ3基因作为动态刺激。
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