公开(公告)号：KR101097882B1

公开(公告)日：2011-12-23

申请号：KR1020090091123

申请日：2009-09-25

Applicant: 한국과학기술원

Inventor： 박현규 ,  이진우 ,  김문일 ,  심종민 ,  이태화

IPC: C12N11/14 ,  G01N33/552

Abstract: 본발명에따른다공성실리카복합체는다양한질병의마커가되는포도당, 갈락토스, 콜레스테롤등의소분자물질, 단백질, DNA, 병원균등을민감하게진단할수 있고, 자성나노입자및 효소를다공성실리카에집적함으로써단위개체당활성이높아고감도정량분석이가능하며, 자성나노입자를이용하여효율적인분리와재사용이가능한효과를가진다.

公开(公告)号：KR1020110033575A

公开(公告)日：2011-03-31

申请号：KR1020090091123

申请日：2009-09-25

Applicant: 한국과학기술원

Inventor： 박현규 ,  이진우 ,  김문일 ,  심종민 ,  이태화

IPC: C12N11/14 ,  G01N33/552

Abstract: PURPOSE: A porous silica composite and a method for preparing the same are provided to sensitively diagnose pathogens, small molecular material, proteins, and DNA. CONSTITUTION: A porous silica composite comprises: magnetic nanoparticles with peroxidase activity; and an enzyme which provides a reactive substrate catalyzed by peroxidase or an enzyme using a reactant catalyzed by peroxidase. The substrate catalyzed by hydrogen peroxide is hydrogen peroxide. The reactant catalyzed by peroxidase is water(H_2O) or oxygen(O_2). The porous silica is MSU, SBA, MCM, or KIT. A method for preparing the porous silica composite comprises: a step of precipitating the magnetic particles inside a pore of the silica; and a step of fixing the enzyme into the pore.

Abstract translation: 目的：提供多孔二氧化硅复合物及其制备方法，以敏感地诊断病原体，小分子材料，蛋白质和DNA。 构成：多孔二氧化硅复合材料包括：具有过氧化物酶活性的磁性纳米颗粒; 以及提供由过氧化物酶或酶催化的反应性底物的酶，其使用由过氧化物酶催化的反应物。 由过氧化氢催化的底物是过氧化氢。 由过氧化物酶催化的反应物是水（H_2O）或氧（O_2）。 多孔二氧化硅是MSU，SBA，MCM或KIT。 一种多孔二氧化硅复合体的制造方法，其特征在于，包括使所述二氧化硅的细孔内的磁性粒子析出的工序; 以及将酶固定到孔中的步骤。