公开(公告)号：KR1020010009719A

公开(公告)日：2001-02-05

申请号：KR1019990028251

申请日：1999-07-13

Applicant: 한국과학기술원

Inventor： 장용근 ,  류희욱 ,  이은교 ,  조경숙

IPC: C12P7/62

Abstract: PURPOSE: A method for separating polyhydroxy-alkanoate from a microorganism is provided, thereby the installation cost and operating cost can be lowered, and polyhydroxy-alkanoate is simply separated in higher yield and purity, from a microorganism containing polyhydroxy-alkanoate in its body, with generating a little amount of liquid waste. CONSTITUTION: The method for separating polyhydroxy-alkanoate from a microorganism comprises the steps of: dissolving the cell components of a microorganism by adding SDS into the microorganism fermented culture containing polyhydroxy-alkanoate, and heating the solution with agitation to 50 to 150deg.C; recovering polyhydroxy-alkanoate from the treated solution; washing the recovered polyhydroxy-alkanoate with solvent; and drying the washed polyhydroxy-alkanoate, in which microorganism is selected from Alcaligenes latus, Alcaligenes eutrophus, Azotobacter vinelandii, Escherichia coli, Pseudomonas spp., and Methylotrophs, and SDS is added in an amount of 0.3 to 0.5g per 1g of dried microorganism containing polyhydroxy-alkanoate.

Abstract translation: 目的：提供从微生物中分离多羟基 - 链烷酸酯的方法，从而降低了安装成本和操作成本，并且从其在其体内含有聚羟基 - 链烷酸酯的微生物中以更高的产率和纯度简单地分离出多羟基 - 链烷酸酯， 产生少量的废液。 构成：从微生物分离多羟基链烷酸酯的方法包括以下步骤：通过将SDS加入到含有多羟基 - 链烷酸酯的微生物发酵培养基中，并将该溶液搅拌加热至50〜150℃，从而溶解微生物的细胞成分; 从处理的溶液中回收多羟基 - 链烷酸酯; 用溶剂洗涤回收的聚羟基链烷酸酯; 并干燥洗涤过的多羟基 - 链烷酸酯，其中微生物选自产碱杆菌，产碱杆菌，维生素Azotobacter vinelandii，大肠杆菌，假单胞菌属和甲状腺营养素，SDS以0.3至0.5g / 1g干燥微生物 含有多羟基 - 链烷酸酯。