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1.发明授权유기황 화합물이 함유된 화석연료를 탈황시키는 신규의 크렙시엘라 옥시토카 균주 및 그를 이용한 탈황방법 失效一种新型的KLEBISIELLA OXYTOCA，其从含有机硫化合物和脱硫方法的氟系燃料中除去硫
公开(公告)号：KR100205255B1
公开(公告)日：1999-07-01
申请号：KR1019970009576
申请日：1997-03-20
Applicant: 한국과학기술원
IPC: C12N1/20
Abstract: 본 발명은 유기황 화합물을 탈황시키는 신규의 크렙시엘라 옥시토카(Klebsiella oxytoca) 균주 및 그를 이용한 탈황방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 석유와 석탄과 같은 화석연료내에 존재하는 황 함유의 유기타소 화합물로부터 황을 제거할 수 있는 신규의 미생물인 크렙시엘라 옥시토카 균주 및 그를 이용하여 화석연료내의 황 성분 또는 방향족 화합물을 제거하는 생물학적 방법에 관한 것이다. 본 발명의 분리균주는 고농도의 디벤조티오펜, 예를 들면 헥사데칸/디벤조티오펜 용해계나 유기황 농도가 높은 실제 연료유에 대해서도 효율적인 탈활효과를 나타내고, 종래의 탈황균주와는 달리, 균주생산의 탈황반응시 별도의 첨가물을 요구하지 않아 경제적이며, 바다 신속하게 탈황시킨다는 장점을 가지고 있다. 또한, 전기 분리균주는 유기황 화합물 뿐만 아니라, 기타 방향족 화합물들도 효과적으로 제거한다.
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公开(公告)号：KR1020030034945A
公开(公告)日：2003-05-09
申请号：KR1020010066772
申请日：2001-10-29
Applicant: 한국과학기술원
IPC: C10G32/00
Abstract: PURPOSE: A method for recovering a biocatalyst and desulfurization oil from the emulsion generated during the biological petroleum desulfurization process is provided, to recover the desulfurized oil to the amount of 95 % or more without deterioration of the desulfurization performance. CONSTITUTION: A biocatalyst and desulfurization oil are recovered from the emulsion generated during the biological petroleum desulfurization process, by adding an alcohol to the three-phase emulsion of oil-biocatalyst-water and stirring the mixture; and separating the pretreated emulsion into three layers of an oil, a biocatalyst and water. Preferably the biocatalyst is Gordonia nitida CYKS 1 (KCTC0431BP). Also a biocatalyst and desulfurization diesel oil are recovered by adding ethanol to the three-phase emulsion of diesel oil-Gordonia nitida CYKS 1-water to the concentration of 1-4 % (v/v) and stirring the mixture; and separating the pretreated emulsion into three layers of a diesel oil, a Gordonia nitida CYKS 1 biocatalyst and water.
Abstract translation: 目的：提供一种从生物石油脱硫工艺中产生的乳液中回收生物催化剂和脱硫油的方法，将脱硫油回收率达95％以上，脱硫性能下降。 构成：通过在生物石油脱硫工艺中生成的乳液中回收生物催化剂和脱硫油，通过向油 - 生物催化剂 - 水的三相乳液中加入醇并搅拌混合物; 并将预处理的乳液分离成三层油，生物催化剂和水。 优选地，生物催化剂是Gordonia nitida CYKS 1（KCTC0431BP）。 通过向柴油-Gordonia nitida CYKS 1-水的三相乳液中加入乙醇至1-4％（v / v）的浓度并搅拌混合物，回收生物催化剂和脱硫柴油。 并将预处理的乳液分离成三层柴油，Gordonia nitida CYKS 1生物催化剂和水。
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公开(公告)号：KR100301959B1
公开(公告)日：2001-10-29
申请号：KR1019990017483
申请日：1999-05-15
Applicant: 한국과학기술원
Abstract: 본발명은철산화미생물을이용하여기체속의황화수소(HS)를제거하는처리과정에있어서, 철산화미생물을배양시킬수 있는철산화반응조; 전기철산화반응조로부터배출되는철산화미생물배양배지와황화수소함유기체를반응시키기위한흡수탑; 전기흡수탑으로부터배출되는배양배지/S슬러리혼합액을분리시키기위한분리기; 전기흡수탑으로부터배출되는배출기체속의잔류황화수소를제거시키기위한바이오필터; 이동중의배양배지를저장시키기위한저장조; 및, 철산화반응조, 흡수탑, 분리기및 저장조로부터배양배지를이동시키기위한펌프를포함하는황화수소(HS) 제거장치및 전기황화수소제거장치를이용하여기체속의황화수소를효율적으로제거하는방법에관한것이다. 본발명에의하면, 기체속의황화수소(HS)를잔류 HS 없이완벽하게제거시킬수 있음은물론, 장기간에걸쳐사용하여도철침전물이생성되지않으므로, 경제적이고효율적으로기체속에함유된황화수소를제거시킬수 있다.
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公开(公告)号：KR100447529B1
公开(公告)日：2004-09-08
申请号：KR1020010066772
申请日：2001-10-29
Applicant: 한국과학기술원
IPC: C10G32/00
Abstract: PURPOSE: A method for recovering a biocatalyst and desulfurization oil from the emulsion generated during the biological petroleum desulfurization process is provided, to recover the desulfurized oil to the amount of 95 % or more without deterioration of the desulfurization performance. CONSTITUTION: A biocatalyst and desulfurization oil are recovered from the emulsion generated during the biological petroleum desulfurization process, by adding an alcohol to the three-phase emulsion of oil-biocatalyst-water and stirring the mixture; and separating the pretreated emulsion into three layers of an oil, a biocatalyst and water. Preferably the biocatalyst is Gordonia nitida CYKS 1 (KCTC0431BP). Also a biocatalyst and desulfurization diesel oil are recovered by adding ethanol to the three-phase emulsion of diesel oil-Gordonia nitida CYKS 1-water to the concentration of 1-4 % (v/v) and stirring the mixture; and separating the pretreated emulsion into three layers of a diesel oil, a Gordonia nitida CYKS 1 biocatalyst and water.
Abstract translation: 目的：提供一种从生物石油脱硫过程中产生的乳液中回收生物催化剂和脱硫油的方法，以在不使脱硫性能劣化的情况下将脱硫油回收到95％或更多的量。 构成：生物催化剂和脱硫油从生物石油脱硫过程中产生的乳液中回收，向油 - 生物催化剂 - 水的三相乳液中加入醇并搅拌混合物; 并将预处理的乳液分成三层油，生物催化剂和水。 生物催化剂优选为Gordonia nitida CYKS1（KCTC0431BP）。 通过向柴油Gordonia nitida CYKS 1-水的三相乳液中加入乙醇至1-4％（v / v）的浓度并搅拌混合物来回收生物催化剂和脱硫柴油; 并将经预处理的乳液分离成三层柴油，Gordonia nitida CYKS 1生物催化剂和水。
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Patent Agency Ranking
公开(公告)号：KR1020010009719A
公开(公告)日：2001-02-05
申请号：KR1019990028251
申请日：1999-07-13
Applicant: 한국과학기술원
IPC: C12P7/62
Abstract: PURPOSE: A method for separating polyhydroxy-alkanoate from a microorganism is provided, thereby the installation cost and operating cost can be lowered, and polyhydroxy-alkanoate is simply separated in higher yield and purity, from a microorganism containing polyhydroxy-alkanoate in its body, with generating a little amount of liquid waste. CONSTITUTION: The method for separating polyhydroxy-alkanoate from a microorganism comprises the steps of: dissolving the cell components of a microorganism by adding SDS into the microorganism fermented culture containing polyhydroxy-alkanoate, and heating the solution with agitation to 50 to 150deg.C; recovering polyhydroxy-alkanoate from the treated solution; washing the recovered polyhydroxy-alkanoate with solvent; and drying the washed polyhydroxy-alkanoate, in which microorganism is selected from Alcaligenes latus, Alcaligenes eutrophus, Azotobacter vinelandii, Escherichia coli, Pseudomonas spp., and Methylotrophs, and SDS is added in an amount of 0.3 to 0.5g per 1g of dried microorganism containing polyhydroxy-alkanoate.
Abstract translation: 目的：提供从微生物中分离多羟基 - 链烷酸酯的方法，从而降低了安装成本和操作成本，并且从其在其体内含有聚羟基 - 链烷酸酯的微生物中以更高的产率和纯度简单地分离出多羟基 - 链烷酸酯， 产生少量的废液。 构成：从微生物分离多羟基链烷酸酯的方法包括以下步骤：通过将SDS加入到含有多羟基 - 链烷酸酯的微生物发酵培养基中，并将该溶液搅拌加热至50〜150℃，从而溶解微生物的细胞成分; 从处理的溶液中回收多羟基 - 链烷酸酯; 用溶剂洗涤回收的聚羟基链烷酸酯; 并干燥洗涤过的多羟基 - 链烷酸酯，其中微生物选自产碱杆菌，产碱杆菌，维生素Azotobacter vinelandii，大肠杆菌，假单胞菌属和甲状腺营养素，SDS以0.3至0.5g / 1g干燥微生物 含有多羟基 - 链烷酸酯。
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7.发明公开
公开(公告)号：KR1019980073969A
公开(公告)日：1998-11-05
申请号：KR1019970009576
申请日：1997-03-20
Applicant: 한국과학기술원
IPC: C12N1/20
Abstract: 본 발명은 유기황 화합물을 탈황시키는 신규의 크렙시엘라 옥시토카(Klebsiella oxytoca) 균주 및 그를 이용한 탈황방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 석유와 석탄과 같은 화석연료내에 존재하는 황 함유의 유기탄소 화합물로부터 황을 제거할 수 있는 신규의 미생물인 크렙시엘라 옥시토카 균주 및 그를 이용하여 화석연료내의 황 성분 또는 방향족 화합물을 제거하는 생물학적 방법에 관한 것이다. 본 발명의 분리균주는 고농도의 디벤조티오펜, 예를 들면 헥사데칸/디벤조티오펜 용해계나 유기황 농도가 높은 실제 연료유에 대해서도 효율적인 탈황효과를 나타내고, 종래의 탈황균주와는 달리, 균주생산 및 탈황반응시 별도의 첨가물을 요구하지 않아 경제적이며, 보다 신속하게 탈황시킨다는 장점을 가지고 있다. 또한, 전기 분리균주는 유기황 화합물 뿐만 아닐, 기타 방향족 화합물들도 효과적으로 제거한다.
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公开(公告)号：KR1020000073901A
公开(公告)日：2000-12-05
申请号：KR1019990017483
申请日：1999-05-15
Applicant: 한국과학기술원
CPC classification number: B01D53/52 , B01D25/12 , B01D53/1468 , B01D53/84 , B04B1/00
Abstract: PURPOSE: A sulfuretted hydrogen removing device and a method for removing sulfuretted hydrogen are provided which are able to economically and effectively remove sulfuretted hydrogen contained in gas. CONSTITUTION: The device comprises: (i) an iron oxidation reacting tank which is able to cultivate iron oxidized microorganism; (ii) an absorbing tank which reacts the iron oxidized microorganism cultivated medium with gas containing sulfuretted hydrogen; (iii) a separator which is to separate a mixture solution of the cultivated medium/S0 slurry discharged from the absorbing tank; (iv) a bio-filter which is to remove the residual sulfuretted hydrogen contained in the discharge gas discharged from the absorbing tank; (v) a storage tank which is to store the cultivated medium discharged from the reacting tank, the absorbing tank and the separator; and (vi) a pump which is to transfer the cultivated medium from the iron oxidation reacting tank, absorbing tank, separator and the storage tank. The method comprises steps of: (i) producing an iron oxide solution by cultivating an iron oxidation microorganism in an iron oxidation reacting tank containing a fibrous microorganism fixation carrier, where the iron oxidation microorganism forms a biological film, and an iron oxidation microorganism cultivating medium; (ii) contacting the iron oxide solution with gas containing sulfuretted hydrogen to oxidize the sulfuretted hydrogen contained in the gas into a slurry of S0 and reduce the iron oxide solution into reduced iron solution; (iii) discharging the produced slurry of S0 and the reduced iron solution from the absorbing tank and separating them by using a separator; (iv) adjusting pH of the separated reduced iron solution into 1.7 to 2.3 by adding lime or calcium carbonate and then re-injecting the pH-adjusted reduced iron solution to the iron oxidation reacting tank to re-use it as a cultivation medium for producing an iron oxide solution; and (v) injecting discharge gas discharged from the absorbing tank into a bio-filter containing a carrier, where sulfated microorganisms are fixed on, to remove residual sulfuretted hydrogen contained in the gas.
Abstract translation: 目的：提供一种硫化氢去除装置和一种去除硫化氢的方法，其能够经济有效地除去气体中所含的硫化氢。 构成：该装置包括：（i）能够培养铁氧化微生物的铁氧化反应槽; （ii）使铁氧化微生物培养基与含有硫化氢的气体反应的吸收罐; （iii）分离从吸收罐排出的栽培培养基/ S0浆料的混合溶液的分离器; （iv）生物过滤器，用于去除从吸收罐排出的排出气体中所含的残留的硫化氢; （v）存储从反应罐，吸收罐和分离器排出的培养基的储存罐; 和（vi）将来自铁氧化反应槽，吸收罐，分离器和储罐的培养介质转移的泵。 该方法包括以下步骤：（i）通过在含有铁氧化微生物形成生物膜的纤维微生物固定载体的铁氧化反应槽中培养铁氧化微生物和铁氧化微生物栽培培养基来生产氧化铁溶液 ; （ii）使氧化铁溶液与含有硫化氢的气体接触，将气体中所含的硫化氢氧化成S0的浆料，并将氧化铁溶液还原成还原铁溶液; （iii）从所述吸收罐排出所产生的S0浆料和还原铁溶液，并使用分离器将其分离; （iv）通过加入石灰或碳酸钙将分离的还原铁溶液的pH调节至1.7至2.3，然后将pH调节的还原铁溶液重新注入到铁氧化反应槽中，以再次将其用作生产的培养基 氧化铁溶液; 和（v）将从吸收罐排出的排放气体注入含有硫酸化微生物固定在其上的载体的生物过滤器中，以除去气体中所含的残留的硫化氢。
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公开(公告)号：KR1019940010108B1
公开(公告)日：1994-10-21
申请号：KR1019910015018
申请日：1991-08-29
Applicant: 한국과학기술원
IPC: B01J10/00
CPC classification number: B01J10/002 , C12M29/04
Abstract: The reactor (1) comprises a bubble tower (4), a cylinder-type radial air-dispense (1), a air-dispenser mount (3), air-outlet (9) and a reactor cover (8) having two inlets (10) for sensor and raw materials. The ratio of height to diameter of the bubble tower reactor (1) is more than 10:1 and the surface area of the air-dispenser is more than 1.6 times as the wide as sectional area of the bubble tower. This reactor is useful for biological reaction or air phase-liquid phase reaction.
Abstract translation: 反应器（1）包括气泡塔（4），圆柱型径向分配气（1），空气分配器支架（3），空气出口（9）和具有两个入口的反应器盖 （10）传感器和原材料。 泡沫塔式反应器（1）的高度与直径的比例大于10:1，空气分配器的表面积比气泡塔的截面面积的宽度大1.6倍。 该反应器可用于生物反应或空气相 - 液相反应。
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