-
公开(公告)号：KR1020110020468A
公开(公告)日：2011-03-03
申请号：KR1020090078088
申请日：2009-08-24
Applicant: 한국과학기술원
CPC classification number: C12N5/0606 , C12N5/0662 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/415 , C12N2501/999 , C12N2506/02
Abstract: PURPOSE: A method for differentiating mesenchymal stem cells from human embryonic stem cells is provided to obtain a large amount of mesenchymal stem cells used in cell therapy. CONSTITUTION: A composition for inducing embryonic stem cell(hESC)-derived mesoderm differentiation contains Activin A, 6-bromoindirubin-3'-oxime(BIO), and BMP4. Activin A, 6-bromoindirubin-3'-oxime(BIO), and BMP4 are contained in a final medium in a concentration of 2-5 ng/ml, 2-5 mM, and 10-20 ng/ml, respectively. A method for differentiating hESC-derived mesoderm precursor comprises: a step of culturing hESC in a conditioned medium for 1-3 days; and a step of culturing ESCs in the final medium for 3-5 days.
Abstract translation: 目的：提供一种区分间充质干细胞与人类胚胎干细胞的方法，以获得大量用于细胞治疗的间充质干细胞。 构成：用于诱导胚胎干细胞（hESC）衍生的中胚层分化的组合物含有活化素A，6-溴靛蓝素-3'-肟（BIO）和BMP4。 活化素A，6-溴靛玉红-3'-肟（BIO）和BMP4分别以2-5ng / ml，2-5mM和10-20ng / ml的浓度包含在最终培养基中。 用于分离hESC衍生的中胚层前体的方法包括：在条件培养基中培养1-5天的hESC的步骤; 以及在最终培养基中培养ESCs 3-5天的步骤。
-
公开(公告)号：KR101193541B1
公开(公告)日：2012-10-22
申请号：KR1020090078088
申请日：2009-08-24
Applicant: 한국과학기술원
CPC classification number: C12N5/0606 , C12N5/0662 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/415 , C12N2501/999 , C12N2506/02
Abstract: 본 발명은 조성물이 최적화된 배지에서 배양함으로써 인간 배아줄기세포를 중간엽 줄기세포로 분화시키는 방법에 관한 것으로, 구체적으로 TGF-β(Transforming growth factor-β), WNT 및 BMP(Bone morphogenic protein) 신호전달 경로를 활성화함으로써 중배엽을 유도한 후, bFGF 및/또는 PDGF-BB(Platelet-derived growth factor beta polypeptide)의 처리에 의해 중간엽 줄기세포를 제조하는 방법에 관한 것이다. 본 발명의 방법은 세포기반 치료에 유용한 중간엽 줄기세포를 대량으로 제공하는 데에 유용하게 이용될 수 있다.
중간엽 줄기세포, 중배엽, 인간 배아줄기세포, TGF-β(Transforming growth factor-β), WNT, BMP(Bone morphogenic protein), bFGF, PDGF-BB(Platelet-derived growth factor beta polypeptide)
-
Search Results
2
records
(took 0.016 seconds)
