公开(公告)号：KR100876657B1

公开(公告)日：2009-01-07

申请号：KR1020070103729

申请日：2007-10-15

Applicant: 한국기계연구원 ,  재단법인서울대학교산학협력재단

Inventor： 박상진 ,  현진호 ,  이종환 ,  김옥진 ,  김덕종 ,  고득용

IPC: G01N33/48 ,  G01N33/96

Abstract: A method for preparing a surface-modified microbead, a method for preparing a microbead array chip, and a method for checking the disease specific antigen by using the microbead array chip are provided to develop the smart system using elastin-like polypeptide (ELP). A method for preparing a surface-modified microbead comprises the steps of synthesizing the DNA base sequence by PCR based on the [(VGVPG)14(KGVPG)] trimer amino acid sequence to make an ELP expression recombinant vector, and transforming it to E. coli BLR(DE3) to make the biosynthesized ELP; and immobilizing the ELP on the surface of a microbead(40).

Abstract translation: 提供了制备表面改性微珠的方法，微珠阵列芯片的制备方法以及使用微珠阵列芯片检测疾病特异性抗原的方法，以开发使用弹性蛋白样多肽（ELP）的智能系统。 制备表面改性微珠的方法包括以下步骤：基于[（VGVPG）14（KGVPG）]三聚体氨基酸序列通过PCR合成DNA碱基序列以制备ELP表达重组载体，并将其转化为E. 大肠杆菌BLR（DE3）进行生物合成的ELP; 并将ELP固定在微珠（40）的表面上。