公开(公告)号：KR101267250B1

公开(公告)日：2013-05-31

申请号：KR1020110067331

申请日：2011-07-07

Applicant: 한국식품연구원

Inventor： 이나리 ,  최순영 ,  설자영 ,  구민선 ,  전향숙 ,  김현정 ,  장현주

IPC: C12N15/11 ,  C12N15/63 ,  G01N33/53 ,  G01N33/15

Abstract: 본발명은렙틴수용체프로모터및 이와작동가능하게연결된리포터유전자를포함하는비만또는당뇨치료제탐색용재조합발현벡터및 이를이용한비만또는당뇨치료제탐색방법에관한것이다. 본발명에따르면, 비만또는당뇨치료제를검색할수 있는렙틴수용체프로모터서열에리포터유전자가부착된발현벡터를구축할수 있으며, 구축된발현벡터이형질전환된세포주로부터렙틴수용체가발현되는양을확인함으로써, 비만또는당뇨치료제를탐지할수 있음을확인하였다.

公开(公告)号：KR1020130010040A

公开(公告)日：2013-01-24

申请号：KR1020110067331

申请日：2011-07-07

Applicant: 한국식품연구원

Inventor： 이나리 ,  최순영 ,  설자영 ,  구민선 ,  전향숙 ,  김현정 ,  장현주

IPC: C12N15/11 ,  C12N15/63 ,  G01N33/53 ,  G01N33/15

Abstract: PURPOSE: A recombinant expression vector for searching a therapeutic agent for treating obesity or diabetes and a searching method using the same are provided to develop the therapeutic agent. CONSTITUTION: A leptin receptor promoter has a base of sequence number 2. The promoter contains a base selected from sequence numbers 3-15. The promoter contains a base of sequence number 9 or 1. A recombinant vector contains the promoter and a reporter gene. The reporter gene is luciferase, HRP(horseraddish peroxidase) or alkaline phosphatase. A cell line transformed with the recombinant vector is ORp-HEK293(KCTC 11920BP). A method for searching a therapeutic agent for treating obesity or diabetes comprises: a step of treating a candidate material for treating obesity or diabetes to a cell line and a step of confirming expression level of the leptin receptor.

Abstract translation: 目的：提供用于搜索治疗剂用于治疗肥胖或糖尿病的重组表达载体和使用其的检索方法以开发治疗剂。 构成：瘦素受体启动子具有序列号2的碱基。启动子含有选自序列号3-15的碱基。 启动子含有序列号9或1的碱基。重组载体含有启动子和报告基因。 报道基因是荧光素酶，HRP（辣根过氧化物酶）或碱性磷酸酶。 用重组载体转化的细胞系是ORp-HEK293（KCTC 11920BP）。 用于搜索治疗剂用于治疗肥胖或糖尿病的方法包括：将治疗肥胖症或糖尿病的候选物质治疗至细胞系的步骤和确认瘦素受体表达水平的步骤。