公开(公告)号：KR100423551B1

公开(公告)日：2004-03-18

申请号：KR1020010035945

申请日：2001-06-22

Applicant: 한국식품연구원

Inventor： 김영붕 ,  노정해 ,  성기승 ,  한찬규 ,  손동화 ,  이남형 ,  백반석 ,  신태범 ,  고성찬 ,  허강준

IPC: A61K39/40

Abstract: PURPOSE: A Method for the production of an egg containing anti-Edwardsiella tarda IgY, anti-Streptococcus iniae IgY and Mycobacterium bovis IgY simultaneously, an egg produced thereby and a fish feed containing specific IgY thereof are provided. The produced egg and fish feed have excellent prevention effect on a flatfish disease. CONSTITUTION: An emulsion containing Edwardsiella tarda IgY, anti-Streptococcus iniae IgY, Mycobacterium bovis IgY and aluminium oxide in ratio of 3.0:3.0:1.0:3.0 is inoculated into a chicken in the amount of 1.0ml one time, and then, from a second time, the above emulsion and an adjuvant(ISA25) are inoculated together thereinto in the amount of 1.0ml at intervals of 2 weeks to produce an egg containing specific IgY. Egg yolk is then put up in a vessel, stirred in the equal amount of alkali ion water(pH 10.0), left alone for a specified period of time and then the supernatant is ultra-filtrated and freeze-dried after removing a fat layer floated on the upper layer to produce soluble IgY powder.

Abstract translation: 目的：提供一种同时生产含抗爱德华兹氏艾美尔球虫IgY，抗链球菌IgY和牛分枝杆菌IgY的蛋，由此产生的蛋和含有特定IgY的鱼饲料的方法。 生产的鸡蛋和鱼饲料对flat鱼疾病具有极好的预防效果。 构成：将含有比率为3.0:3.0:1.0:3.0的爱德华氏菌爱德华氏菌IgY，抗茵链球菌IgY，牛分枝杆菌IgY和氧化铝的乳剂一次接种到鸡1.0ml中，然后从 第二次以2周的间隔将上述乳剂和佐剂（ISA25）以1.0ml的量接种在其中，以产生含有特异性IgY的鸡蛋。 然后将蛋黄放入容器中，在等量的碱离子水（pH10.0）中搅拌，静置一段特定时间，然后将上清液超滤并在除去漂浮的脂肪层后冷冻干燥 在上层产生可溶性IgY粉末。

公开(公告)号：KR1020030000261A

公开(公告)日：2003-01-06

申请号：KR1020010035945

申请日：2001-06-22

Applicant: 한국식품연구원

Inventor： 김영붕 ,  노정해 ,  성기승 ,  한찬규 ,  손동화 ,  이남형 ,  백반석 ,  신태범 ,  고성찬 ,  허강준

IPC: A61K39/40

Abstract: PURPOSE: A Method for the production of an egg containing anti-Edwardsiella tarda IgY, anti-Streptococcus iniae IgY and Mycobacterium bovis IgY simultaneously, an egg produced thereby and a fish feed containing specific IgY thereof are provided. The produced egg and fish feed have excellent prevention effect on a flatfish disease. CONSTITUTION: An emulsion containing Edwardsiella tarda IgY, anti-Streptococcus iniae IgY, Mycobacterium bovis IgY and aluminium oxide in ratio of 3.0:3.0:1.0:3.0 is inoculated into a chicken in the amount of 1.0ml one time, and then, from a second time, the above emulsion and an adjuvant(ISA25) are inoculated together thereinto in the amount of 1.0ml at intervals of 2 weeks to produce an egg containing specific IgY. Egg yolk is then put up in a vessel, stirred in the equal amount of alkali ion water(pH 10.0), left alone for a specified period of time and then the supernatant is ultra-filtrated and freeze-dried after removing a fat layer floated on the upper layer to produce soluble IgY powder.

Abstract translation: 目的：提供一种产生含有抗Edwardsiella tarda IgY，抗链球菌IgY和牛分枝杆菌IgY的蛋的方法，由此产生的蛋和含有其特异性IgY的鱼饲料。 生产的鸡蛋和鱼饲料对鲽鱼病具有极好的预防作用。 构成：将含有Edwardsiella tarda IgY，抗链球菌IgY，牛分枝杆菌IgY和氧化铝的比例为3.0:3.0:1.0:3.0的乳剂一次接种到鸡1.0ml中，然后从 第二次，将上述乳剂和佐剂（ISA25）以2周的间隔以1.0ml的量接种在一起，以产生含有特异性IgY的蛋。 然后将蛋黄放在容器中，在等量的碱性离子水（pH10.0）中搅拌一段时间，然后将上清液超滤并冷冻干燥，除去脂肪层漂浮 在上层产生可溶性IgY粉末。