公开(公告)号：KR101075580B1

公开(公告)日：2011-10-20

申请号：KR1020080111458

申请日：2008-11-11

Applicant: 한국해양연구원

Inventor： 이홍금 ,  임정한 ,  김인희 ,  박하주 ,  강필성 ,  김성진 ,  김주영 ,  김일찬 ,  홍순규 ,  김덕규 ,  이유경 ,  이성구

IPC: C12N15/56 ,  C12N9/24 ,  C12N15/09

Abstract: 본발명에따른엑소키티나아제는저온을포함한다양한온도및 pH 범위에서높은활성을나타내고, 다양한금속이온의존재하에서도안정적으로효소활성을유지하고있어, 키틴과키틴올리고머를이용하여키틴모노머의생산에유용하다.

公开(公告)号：KR1020100052650A

公开(公告)日：2010-05-20

申请号：KR1020080111458

申请日：2008-11-11

Applicant: 한국해양연구원

Inventor： 이홍금 ,  임정한 ,  김인희 ,  박하주 ,  강필성 ,  김성진 ,  김주영 ,  김일찬 ,  홍순규 ,  김덕규 ,  이유경 ,  이성구

IPC: C12N15/56 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: A novel exochitinase isolated from Pseudoalteromonas issachenkonii is provided to ensure high activity at wide temperature and pH concentration range and to maintain enzyme activity. CONSTITUTION: An exochitinase has an amino acid sequence of sequence number 1. The exochitinase is derived from Pseudoalteromonas issachenkonii KOPRI 22718(KCTC 11415BP). An exochitinase has a nucleotide sequence of sequence number 2. A recombinant vector contains the exochitinase gene. A recombinant microorganism is obtained by inserting exochitinase gene to host cell chromosome or introducing the recombinant vector to a host cell. The exochitinase is obtained by culturing the recombinant microorganism.

Abstract translation: 目的：提供从假假单胞菌属假单胞菌分离得到的新型外切蛋白酶，以确保在宽温度和pH浓度范围内的高活性，并保持酶活性。 构成：外切蛋白酶具有序列号1的氨基酸序列。外切蛋白酶衍生自假金黄色葡萄球菌KOPRI 22718（KCTC 11415BP）。 外切蛋白酶具有序列号2的核苷酸序列。重组载体含有外切蛋白酶基因。 通过将外切蛋白酶基因插入宿主细胞染色体或将重组载体引入宿主细胞获得重组微生物。 通过培养重组微生物获得外切蛋白酶。

公开(公告)号：KR100732250B1

公开(公告)日：2007-06-27

申请号：KR1020060015721

申请日：2006-02-17

Applicant: 한국해양연구원

Inventor： 이홍금 ,  임정한 ,  김인희 ,  이유경 ,  김일찬 ,  홍순규

IPC: C12N15/31

Abstract: A novel endochitinase and Sanguibacter sp. producing the same endochitinase are provided to show high activity of the endochitinase at various temperature including cool temperature and pH ranges, and stable activity in the presence of surfactants, so that the novel endochitinase and its producing microorganism are useful for production of chitinoligomer from chitin. A gene encoding the novel endochitinase has the nucleotide sequence of SEQ ID NO:1. The Sanguibacter sp. KCTC 10714BP produces the novel endochitinase encoded by the gene of SEQ ID NO:1. The novel endochitinase is produced by (a) culturing Sanguibacter sp. KCTC 10714BP or transformed Escherichia coli containing the endochitinase gene in a medium containing 0.1-1% of chitin as a carbon source at 4-25 deg.C, and (b) recovering the novel endochitinase from the cultured medium.

Abstract translation: 一种新的内切几丁质酶和Sanguibacter sp。 提供了产生相同的内切几丁质酶，以在包括冷温度和pH范围的各种温度下表现出内切壳多糖酶的高活性，并且在表面活性剂存在下具有稳定的活性，从而新型内切几丁酶及其产生微生物可用于从几丁质生产壳寡糖低聚物。 编码新内切壳多糖酶的基因具有SEQ ID NO：1的核苷酸序列。 Sanguibacter sp。 KCTC 10714BP产生由SEQ ID NO：1的基因编码的新型内切几丁质酶。 通过（a）培养Sanguibacter sp。产生新内切壳多糖酶。 KCTC 10714BP或转化的含有内切壳多糖酶基因的大肠杆菌在含有0.1-1％几丁质作为碳源的培养基中，4-25℃，和（b）从培养基中回收新的内切几丁质酶。