公开(公告)号：KR1020100052650A

公开(公告)日：2010-05-20

申请号：KR1020080111458

申请日：2008-11-11

Applicant: 한국해양연구원

Inventor： 이홍금 ,  임정한 ,  김인희 ,  박하주 ,  강필성 ,  김성진 ,  김주영 ,  김일찬 ,  홍순규 ,  김덕규 ,  이유경 ,  이성구

IPC: C12N15/56 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: A novel exochitinase isolated from Pseudoalteromonas issachenkonii is provided to ensure high activity at wide temperature and pH concentration range and to maintain enzyme activity. CONSTITUTION: An exochitinase has an amino acid sequence of sequence number 1. The exochitinase is derived from Pseudoalteromonas issachenkonii KOPRI 22718(KCTC 11415BP). An exochitinase has a nucleotide sequence of sequence number 2. A recombinant vector contains the exochitinase gene. A recombinant microorganism is obtained by inserting exochitinase gene to host cell chromosome or introducing the recombinant vector to a host cell. The exochitinase is obtained by culturing the recombinant microorganism.

Abstract translation: 目的：提供从假假单胞菌属假单胞菌分离得到的新型外切蛋白酶，以确保在宽温度和pH浓度范围内的高活性，并保持酶活性。 构成：外切蛋白酶具有序列号1的氨基酸序列。外切蛋白酶衍生自假金黄色葡萄球菌KOPRI 22718（KCTC 11415BP）。 外切蛋白酶具有序列号2的核苷酸序列。重组载体含有外切蛋白酶基因。 通过将外切蛋白酶基因插入宿主细胞染色体或将重组载体引入宿主细胞获得重组微生物。 通过培养重组微生物获得外切蛋白酶。

公开(公告)号：KR101075580B1

公开(公告)日：2011-10-20

申请号：KR1020080111458

申请日：2008-11-11

Applicant: 한국해양연구원

Inventor： 이홍금 ,  임정한 ,  김인희 ,  박하주 ,  강필성 ,  김성진 ,  김주영 ,  김일찬 ,  홍순규 ,  김덕규 ,  이유경 ,  이성구

IPC: C12N15/56 ,  C12N9/24 ,  C12N15/09

Abstract: 본발명에따른엑소키티나아제는저온을포함한다양한온도및 pH 범위에서높은활성을나타내고, 다양한금속이온의존재하에서도안정적으로효소활성을유지하고있어, 키틴과키틴올리고머를이용하여키틴모노머의생산에유용하다.