公开(公告)号：KR1020090111894A

公开(公告)日：2009-10-28

申请号：KR1020080037494

申请日：2008-04-23

Applicant: 한림대학교 산학협력단

Inventor： 최수영 ,  김대원 ,  장상호 ,  최진희 ,  음원식 ,  강태천 ,  권오신 ,  박진서

IPC: C07K19/00

CPC classification number: C12N9/1223 ,  A61K38/45 ,  A61K47/64 ,  C07K2319/01 ,  C12Y207/03002

Abstract: PURPOSE: A cell permeable creatine kinase fusion protein is provided to effectively treat epilepsy, Alzheimer disease, Parkinson disease and heart disease. CONSTITUTION: A cell permeable creatine kinase fusion protein is bound with a transport domain at the end of one side of creatine kinase by covalent bond. The transport domain comprises 9 to 15 of amino acid residues. The transport domain contains 3/4 or more of arginine or lysine residue. The transport domain is HIV Tat 49-57 residue, oligolysine, oligoarginine or oligo. The fusion protein has an amino acid of the sequence number 7.

Abstract translation: 目的：提供细胞渗透性肌酸激酶融合蛋白，有效治疗癫痫，阿尔茨海默病，帕金森病和心脏病。 构成：细胞渗透性肌酸激酶融合蛋白通过共价键与肌酸激酶一侧末端的转运结构域结合。 转运结构域包含9-15个氨基酸残基。 转运结构域含有3/4以上的精氨酸或赖氨酸残基。 运输区域是HIV Tat 49-57残基，寡聚赖氨酸，寡精氨酸或寡核苷酸。 融合蛋白具有序列号7的氨基酸。

公开(公告)号：KR1020090026838A

公开(公告)日：2009-03-16

申请号：KR1020070091867

申请日：2007-09-11

Applicant: 한림대학교 산학협력단 ,  재단법인춘천바이오산업진흥원

Inventor： 최수영 ,  음원식 ,  박진서 ,  김대원 ,  최진희 ,  장상호 ,  고인영 ,  강일준 ,  원무호 ,  권형주 ,  임순성

IPC: A61K31/7048 ,  A61K31/702

CPC classification number: A61K31/704 ,  A61K36/25

Abstract: A 3-O-(beta-D-glucopyranosyl(1->4)-alpha-L-arabinopyranosyl)-hederagenin is provided to be applied to aiding agent, medicine, cosmetic material increasing infiltration efficiency of the cell permeability fusion protein including Tat-SOD etc. and various disease treating field. A 3-O-(beta-D-glucopyranosyl(1->4)-alpha-L-arabinopyranosyl)-hederagenin improves cell permeation rate of cell permeability fusion protein. The cell permeability fusion protein is formed by covalent bonding HIV-Tat(49-57) consisting of 6~15 amino acids, oligolysine, oligoarginine, oligo(lysine and arginine), protein transport domain selected from PEP-1 and cell non-porous protein. The cell permeability fusion protein is HIV-Tat-Cu,Zn superoxide dismutase. The 3-O- (beta-D-glucopyranosyl(1->4)-alpha-L-arabinopyranosyl)-hederagenin is separated from Fatsia japonica.

Abstract translation: 提供3-O-（β-D-吡喃葡萄糖基（1→4）-alpha-L-阿拉伯吡喃糖基）-hederagenin应用于助剂，药物，化妆品，增加细胞渗透性融合蛋白（包括Tat）的渗透效率 -SOD等和各种疾病治疗领域。 3-O-（β-D-吡喃葡萄糖基（1-> 4）-alpha-L-阿拉伯吡喃糖基）-hederagenin提高细胞渗透性融合蛋白的细胞渗透速率。 细胞渗透性融合蛋白是通过共价键合形成的HIV-Tat（49-57）由6〜15个氨基酸，寡聚赖氨酸，寡精氨酸，寡核苷酸（赖氨酸和精氨酸）组成，蛋白质转运结构域选自PEP-1和细胞无孔 蛋白。 细胞渗透性融合蛋白是HIV-Tat-Cu，Zn超氧化物歧化酶。 从Fatsia japonica分离出3-O-（β-D-吡喃葡萄糖基（1-> 4）-alpha-L-阿拉伯吡喃糖基） - 原基因。

公开(公告)号：KR1020090026382A

公开(公告)日：2009-03-13

申请号：KR1020070091301

申请日：2007-09-10

Applicant: 한림대학교 산학협력단

Inventor： 최수영 ,  음원식 ,  박진서 ,  김대원 ,  최진희 ,  장상호

IPC: C07K19/00

Abstract: A cell introduction PTEN fusion protein is provided to improve cell permeation of PTEN(Phosphatase and tensin homologue deleted on chromosome 10) and to treat cancer, death of skin cell and atopy. A cell introduction PTEN fusion protein has amino acid sequences of a sequence number 7, a sequence number 9 or a sequence number 11 and is formed by covalent bonding PEP-1 protein transport domain of a sequence number 3 at one-side end of PTEN(Phosphatase and tensin homologue deleted on chromosome 10) protein. The recombinant polynucleotide has nucleotide sequence of a sequence number 6, a sequence number 8 or a sequence number 10. The cell introduction PTEN fusion protein is coded from the recombinant polynucleotide.

Abstract translation: 提供细胞介导PTEN融合蛋白，以改善PTEN（10号染色体上缺失的磷酸酶和张力蛋白同源物）的细胞渗透，并治疗癌症，皮肤细胞死亡和特应性。 细胞介导PTEN融合蛋白具有序列号7，序列号9或序列号11的氨基酸序列，并且通过在PTEN的一侧共价键合3号序列的PEP-1蛋白转运结构域（ 磷酸酶和张力蛋白同源物在染色体10）上缺失。 重组多核苷酸具有序列号6，序列号8或序列号10的核苷酸序列。细胞介导PTEN融合蛋白从重组多核苷酸编码。

公开(公告)号：KR100998498B1

公开(公告)日：2010-12-06

申请号：KR1020080037494

申请日：2008-04-23

Applicant: 한림대학교 산학협력단

Inventor： 최수영 ,  김대원 ,  장상호 ,  최진희 ,  음원식 ,  강태천 ,  권오신 ,  박진서

IPC: C07K19/00

Abstract: 본 발명은 크레아틴 키나아제 융합 단백질에 관한 것으로서, 좀더 자세히는 크레아틴 키나아제 단백질의 일측 또는 양측 말단에 단백질 수송 도메인을 공유결합시킨 융합 단백질을 제조하여 세포 또는 핵 내로 도입시키는 방법 및 융합 단백질을 이용한 약제학적 조성물, 건강기능성 식품에 관한 것이다.
크레아틴 키나아제(creatine kinase), 간질, 세포투과, 단백질 치료, 융합 단백질