중합효소 연쇄반응용 마이크로 바이오칩 및 이의 제조방법
    3.
    发明授权
    중합효소 연쇄반응용 마이크로 바이오칩 및 이의 제조방법 失效
    用于聚合酶链反应的微生物芯片及其制造方法

    公开(公告)号:KR100756874B1

    公开(公告)日:2007-09-07

    申请号:KR1020060027419

    申请日:2006-03-27

    CPC classification number: B01L9/527 B01L3/5027 C12M1/38 C12Q1/686

    Abstract: A micro bio chip for polymerase chain reaction and a manufacture method thereof are provided to eliminate the additional sample-introducing device and inhibit foam generation caused by the conventional channel type bio chip by conjugating PDMS(polydimethylsiloxane) to glass and reduce the using amount of sample and time, so that efficiency and accuracy of PCR are improved. A micro bio chip for polymerase chain reaction comprises a PDMS chip(10) into which a sample is easily introduced, and a glass chip which is treated by oxygen plasma, regulates the temperature and is conjugated to the PDMS chip, wherein one side of the glass chip has a micro heater consisting of chromium and gold thin film and a heat resistant sensor, a region for applying the electric source to the micro heater, a region for applying the electric current to the heat resistant sensor for measuring resistance, and a region for detecting the electric pressure value generated by relation between the resistance and electric current. The PDMS chip has a reaction tank(11) with the oblique line type micro channel structure.

    Abstract translation: 提供了用于聚合酶链式反应的微生物芯片及其制造方法,以通过将PDMS(聚二甲基硅氧烷)与玻璃结合并减少样品的使用量来消除额外的样品引入装置并抑制由常规通道型生物芯片引起的泡沫生成 和时间,从而提高PCR的效率和准确性。 用于聚合酶链反应的微生物芯片包括容易引入样品的PDMS芯片(10)和用氧等离子体处理的玻璃芯片,调节温度并与PDMS芯片共轭,其中 玻璃芯片具有由铬和金薄膜构成的微加热器和耐热传感器,将电源施加到微加热器的区域,用于将电流施加到用于测量电阻的耐热传感器的区域,以及区域 用于检测由电阻和电流之间的关系产生的电压值。 PDMS芯片具有具有斜线型微通道结构的反应槽(11)。

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