-
公开(公告)号：KR1020100020394A
公开(公告)日：2010-02-22
申请号：KR1020080079155
申请日：2008-08-12
Applicant: 한양대학교 산학협력단
IPC: C12Q1/68
Abstract: PURPOSE: An integrated micro biochip is provided to perform sample pre-treatment and PCR on one chip and reduce labor, cost, time and sample amount. CONSTITUTION: An integrated micro biochip(100) comprises: a sample pre-treatment reaction bath(120) for removing residues from injected sample cells; a PCR container(140) which is formed at the exit of the sample pre-treatment reaction bath and is able to perform PCR; a check valve(160) which is formed between the reaction bath and PCR container and prevents reverse flow of DNA or PCR mixture; a heating unit(180) which regulates temperature of the reaction bath and PCR container; and thin glass chip(102).
Abstract translation: 目的：提供集成的微生物芯片，在一个芯片上进行样品预处理和PCR，降低人工成本，时间和样品量。 构成：集成的微生物芯片（100）包括：用于去除注射的样品池的残留物的样品预处理反应槽（120） PCR容器（140），其形成在样品预处理反应槽的出口处并能够进行PCR; 止回阀（160），其形成在反应槽和PCR容器之间，并防止DNA或PCR混合物的反向流动; 加热单元（180），其调节反应槽和PCR容器的温度; 和薄玻璃芯片（102）。
-
公开(公告)号：KR1020090124311A
公开(公告)日：2009-12-03
申请号：KR1020080050444
申请日：2008-05-29
Applicant: 한양대학교 산학협력단
IPC: C12Q1/68
CPC classification number: B01L7/52 , C12Q1/6806
Abstract: PURPOSE: A microbiochip for pretreatment of sample and a method for extracting DNA using the same are provided to reduce the amount of sample, time, cost, and labor. CONSTITUTION: A micro biochip(100) for pretreatment of a sample comprises: a heating unit which regulates temperature for efficient experiment; a PDMS(polydimethylsiloxane) chip(130) having a channel(132) with a micro-filter for extracting pure DNA; and thin film glass chip(140). The heating unit comprises a glass chip(160) placed at the lower part of the thin film glass chip, micro heater, and temperature sensor(180). A method for extracting DNA using the micro biochip comprise: a step of injecting microbead to the channel in reaction bath; a step of filtering microbead in a micropillar; a step of injecting sample cells to the channel; a step of heating the sample cells with the microheater to break cell wall; and a step of extracting DNA.
Abstract translation: 目的：提供用于预处理样品的微生物芯片和使用其的DNA提取方法，以减少样品量，时间，成本和劳动。 构成：用于预处理样品的微生物芯片（100）包括：加热单元，其调节温度以进行有效的实验; 具有通道（132）的PDMS（聚二甲基硅氧烷）芯片（130），其具有用于提取纯DNA的微滤器; 和薄膜玻璃芯片（140）。 加热单元包括放置在薄膜玻璃芯片，微加热器和温度传感器（180）的下部的玻璃芯片（160）。 使用微生物芯片提取DNA的方法包括：将微珠注入反应槽中的通道的步骤; 在微柱中过滤微珠的步骤; 将样品池注入通道的步骤; 用微量加热器加热样品池以破裂细胞壁的步骤; 和提取DNA的步骤。
-
公开(公告)号：KR1020080007733A
公开(公告)日：2008-01-23
申请号：KR1020060066735
申请日：2006-07-18
Applicant: 한양대학교 산학협력단
IPC: G01N33/53 , G01N33/50 , G01N33/552 , G01N33/554
Abstract: A micro bio-chip for immunoreaction is provided to maximize the reliability and the satisfaction index in use by reducing the amount of a sample used and a testing time and accurately and rapidly measuring a biochemical test. A micro bio-chip for immunoreaction(100) is characterized in that a glass chip(10) for detecting the immunoreaction using an electric signal through a microelectrode(11) and a PDMS chip(20) for injecting a sample for detecting the immunoreaction are placed on the top side of the glass chip and integrated thereinto, wherein the PDMS chip consists of a chamber(21), a microfilter(22) and a channel(23). A method for preparing the micro bio-chip for immunoreaction comprises the steps of: (a) forming the glass chip for detecting the immunoreaction using the electric signal; (b) forming the PDMS chip for injecting the sample such as a bio-chemical material for detecting the immunoreaction; and (c) after connecting the PDMS chip to the top side of the glass chip, treating it with oxygen plasma with constant power for a predetermined time to integrate the PDMS chip into the glass chip. A method for detecting the immunoreaction using the micro bio-chip comprises the steps of: (a) fixing an antigen and an antibody using the micro bio-chip and a micro-bead; and (b) performing to detect the immunoreaction through an electric signal using nano-gold and a silver solution.
Abstract translation: 提供用于免疫反应的微生物芯片，以通过减少使用的量和测试时间并准确且快速地测量生物化学测试来最大化使用中的可靠性和满意度指数。 用于免疫反应的微生物芯片（100）的特征在于，用于通过微电极（11）和用于注射用于检测免疫反应的样品的PDMS芯片（20）检测电信号的免疫反应的玻璃芯片（10） 放置在玻璃芯片的顶侧并集成在其中，其中PDMS芯片由腔室（21），微过滤器（22）和通道（23）组成。 制备用于免疫反应的微生物芯片的方法包括以下步骤：（a）使用电信号形成用于检测免疫反应的玻璃芯片; （b）形成用于注射用于检测免疫反应的生物化学材料的样品的PDMS芯片; 和（c）在将PDMS芯片连接到玻璃芯片的顶部之后，用等离子体等离子体处理一定时间以将PDMS芯片集成到玻璃芯片中。 使用微生物芯片检测免疫反应的方法包括以下步骤：（a）使用微生物芯片和微珠固定抗原和抗体; 和（b）通过使用纳米金和银溶液的电信号来检测免疫反应。
-
公开(公告)号：KR100959399B1
公开(公告)日：2010-05-24
申请号：KR1020080097627
申请日：2008-10-06
Applicant: 한양대학교 산학협력단
IPC: F04B43/04
Abstract: 본 발명은 멤브레인형 플랩을 구비한 분리 가능한 열공압형 마이크로 펌프칩 및 이의 제작방법에 관한 것으로, 더욱 상세하게는 기판 상에 히터와 센서가 형성된 마이크로 히터칩; 상기 마이크로 히터칩과 대면하며, 시료의 유출입을 위해 유로로 연결되며 인렛부 및 아웃렛부와 이들 사이에 펌프챔버를 구비한 마이크로 반응조칩; 및 상기 마이크로 히터칩과 마이크로 반응조칩이 장착된 하우징이 구비된 열공압형 마이크로 펌프칩에 있어서, 상기 마이크로 반응조칩은 유로의 개방/폐쇄를 위해 멤브레인형 플랩(flap)을 구비하고, 상기 마이크로 히터칩과 마이크로 반응조칩은 분리가 가능한 것인 열공압형 마이크로 펌프칩 및 이의 제작방법에 관한 것이다.
상기 열공압형 마이크로 펌프칩은 시료의 역류 현상을 방지하여 측정 오차를 낮춰, 이를 구비한 바이오칩의 신뢰도를 높이고, 분리형으로 제작되어 시료 측정이 끝난 후 별도의 세척과정 없이 새로운 마이크로 반응조칩의 교체 사용이 가능하여 마이크로 반응조칩의 반복 사용이 가능하고, 결과적으로 비용과 시간이 최소화될 뿐만 아니라 저렴한 가격에 의해 대량생산이 가능하게 되는 효과가 있다.
열공압, 분리형, 박막-
公开(公告)号：KR1020090021957A
公开(公告)日：2009-03-04
申请号：KR1020070086890
申请日：2007-08-29
Applicant: 한양대학교 산학협력단
IPC: C12Q1/68
CPC classification number: B01L3/5027 , B01L7/52 , C12Q1/686
Abstract: Provided is a method for manufacturing a chip for a polymerase chain reaction, which includes a disposable micro reaction bath chip and a semi-permanent micro heater chip, to reduce manufacturing costs and time. A method for manufacturing a chip(100) with a micro reaction bath chip(10) and a micro heater chip(20) for a polymerase chain reaction comprises the following steps. The micro reaction bath chip injects a sample for a biochemistry experiment. The micro heater chip heats the sample and controls a temperature of the sample. The micro reaction bath chip is located above the micro heater chip to be separated from the micro heater chip.
Abstract translation: 提供一种用于聚合酶链式反应的芯片的制造方法，其包括一次性微反应槽芯片和半永久性微加热器芯片，以降低制造成本和时间。 利用微反应槽芯片（10）和用于聚合酶链式反应的微加热器芯片（20）制造芯片（100）的方法包括以下步骤。 微反应槽芯片注入生物化学实验样品。 微加热器芯片加热样品并控制样品的温度。 微反应槽芯片位于微加热器芯片上方，与微加热器芯片分离。
-
Patent Agency Ranking
公开(公告)号：KR100866836B1
公开(公告)日：2008-11-04
申请号：KR1020070046013
申请日：2007-05-11
Applicant: 한양대학교 산학협력단
CPC classification number: B01L3/5027 , C12Q1/6862
Abstract: A micro bio-chip for the ligation reaction is provided to perform a mixing process having high efficiency in a structure of a single layer by combination-arranging in a form of a micro-pillar and a micro-nozzle at the micromixer part, to manufacture it easily because a micromixer does not use an actuator, to diminish an installation cost and to generate stabilized biochemical reaction. A micro bio-chip for the ligation reaction is made of 2 layer of structure by integrating polydimethylsiloxane(PDMS) chip and glass(20). The PDMS chip(10) comprises an inlet part, a micro-mixer, a reactor and an outlet part. The micro-mixer mixes a sample injected through the inlet part(11). A reactor ligation-reacts the mixed sample through the micromixer(12). The micromixer comprises a micro pillar and a micro-nozzle.
Abstract translation: 提供了一种用于连接反应的微生物芯片，通过在微型混合器部分上以微柱和微型喷嘴的形式组合配置，进行单层结构的高效率的混合过程，以制造 这很容易，因为微混合器不使用致动器，以减少安装成本并产生稳定的生化反应。 用于连接反应的微生物芯片由聚二甲基硅氧烷（PDMS）芯片和玻璃（20）组成，由2层结构构成。 PDMS芯片（10）包括入口部分，微混合器，反应器和出口部分。 微混合器混合通过入口部分（11）注入的样品。 反应器连接 - 通过微混合器（12）将混合样品反应。 微混合器包括微柱和微喷嘴。
-
公开(公告)号：KR1020080067797A
公开(公告)日：2008-07-22
申请号：KR1020070005147
申请日：2007-01-17
Applicant: 한양대학교 산학협력단
CPC classification number: B81B3/0018 , B81B3/0035 , B81B7/008 , B81B2201/0214 , B81B2201/036 , B81B2201/054 , B81B2201/06
Abstract: A thermo-pneumatic micro pump chip applicable to a micro bio chip and a manufacturing method thereof are provided to increase precision of flow control of a pump by bonding a poly dimethyl siloxane(PDMS) chip with a glass chip integrally. A thermo-pneumatic micro pump chip(100) that is applicable to a micro bio chip comprises a glass chip(10) and a PDMS chip(20) that are integrally bonded to each other. The glass chip is equipped with a heater(11) and a sensor. In addition, the PDMS chip is formed on an upper surface of the glass chip and moves fluid sample in a desired direction.
Abstract translation: 提供了适用于微生物芯片的热气动微型泵芯片及其制造方法，以通过将聚二甲基硅氧烷（PDMS）芯片与玻璃芯片整体结合来提高泵的流量控制精度。 适用于微生物芯片的热气动微型泵芯片（100）包括彼此一体地结合的玻璃芯片（10）和PDMS芯片（20）。 玻璃芯片配有加热器（11）和传感器。 此外，PDMS芯片形成在玻璃芯片的上表面上，并使流体样品沿期望的方向移动。
-
公开(公告)号：KR101088984B1
公开(公告)日：2011-12-01
申请号：KR1020090046431
申请日：2009-05-27
Applicant: 한양대학교 산학협력단
Abstract: 본 발명은 활동성 정자 분리 장치에 관한 것으로서, 정자의 주화성과 주열성을 이용하여 정자 샘플 내의 활동성 정자를 간편하고 효율적으로 선별할 수 있고, 활동성 정자를 분리하기 위한 농도 구배와 온도 구배를 간단하고 콤팩트한 구조로 구현할 수 있다.
활동성 정자 분리 장치, 농도 구배 형성기, 온도 구배 형성기, 활동성 정자, 인공수정, 주열성, 주화성-
公开(公告)号：KR1020100128025A
公开(公告)日：2010-12-07
申请号：KR1020090046431
申请日：2009-05-27
Applicant: 한양대학교 산학협력단
CPC classification number: C12N5/061 , B01L3/5027 , B01L2200/147 , B01L2300/0867 , B01L2300/1827 , C12N2509/10
Abstract: PURPOSE: An apparatus for separating motile sperm is provided to simply and efficiently select motile sperms in a sperm sample and to ensure simple and contact structure. CONSTITUTION: An apparatus(100) for separating motile sperm comprises a gradient concentration former(110) and gradient temperature former(120). The gradient concentration former has an input(112) for adding sperm sample and a separator for separating motile sperms in the sperm sample. The gradient concentration former have gradually increasing concentration from the input to the separator. The gradient concentration former further comprises a microchannel(114) for connecting the input and separator.
Abstract translation: 目的：提供一种分离运动精子的装置，用于简单有效地选择精子样本中的运动精子，并确保简单和接触结构。 构成：用于分离运动精子的装置（100）包括梯度浓缩物（110）和梯度温度调节剂（120）。 梯度浓度仪具有用于添加精子样品的输入（112）和用于分离精子样品中的运动精子的分离器。 梯度浓度成分从输入到分离器的浓度逐渐增加。 梯度浓度变换器还包括用于连接输入和分离器的微通道（114）。
-
公开(公告)号：KR1020100038593A
公开(公告)日：2010-04-15
申请号：KR1020080097627
申请日：2008-10-06
Applicant: 한양대학교 산학협력단
IPC: F04B43/04
Abstract: PURPOSE: A separable thermopneumatic micropump chip with a membrane flap, and a manufacturing method thereof are provided to reduce measurement errors by preventing a sample from flowing backward. CONSTITUTION: A separable thermopneumatic micropump chip comprises a micro heater chip(10), a micro reaction chip(20), and a housing(30). The micro heater chip has a heater and a sensor formed on a substrate. The micro reaction chip is connected to a flow passage, and has an inlet, an outlet, and a pump chamber. The micro heater chip and the micro reaction chip are separated from each other. The micro reaction chip has a membrane flap which opens and closes the flow passage. The micro heater chip and the micro reaction chip are installed in the housing.
Abstract translation: 目的：提供一种具有膜瓣的可分离的热气泡微型芯片及其制造方法，以通过防止样品向后流动来减少测量误差。 构成：可分离的热空气微型泵芯片包括微加热器芯片（10），微反应芯片（20）和壳体（30）。 微加热器芯片具有形成在基板上的加热器和传感器。 微反应芯片连接到流动通道，并具有入口，出口和泵室。 微加热器芯片和微反应芯片彼此分离。 微反应芯片具有打开和关闭流路的膜片。 微加热器芯片和微反应芯片安装在壳体中。
-
-
-
-
-
-
-
-
-
Search Results
15
records
(took 0.026 seconds)
