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    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS
    2.
    发明公开
    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS 失效
    用于实施两步离子束缚测定的试剂和方法

    公开(公告)号：EP0586605A4

    公开(公告)日：1996-01-17

    申请号：EP92914861

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  FICO ROSARIO ,  JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/545 ,  G01N33/538 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/537 ,  G01N33/541 ,  G01N33/543 ,  G01N33/544 ,  G01N33/546 ,  G01N33/551

    CPC classification number: G01N33/538

    Abstract: This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent.

    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE
    6.
    发明专利
    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE 未知

    公开(公告)号：CA2110050C

    公开(公告)日：2004-02-24

    申请号：CA2110050

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  JOU YI-HER ,  STROUPE STEPHEN DENHAM

    IPC: G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/547 ,  G01N33/548

    Abstract: The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyze of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to th e oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyze on the solid phase even in the presence of polyanionic non-specific binding blockers.

    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS
    8.
    发明专利
    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS 未知

    公开(公告)号：CA2109925C

    公开(公告)日：2004-11-09

    申请号：CA2109925

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  FICO ROSARIO ,  JOU YI-HER ,  STROUPE STEPHEN DENHAM

    IPC: G01N33/545 ,  G01N33/538 ,  G01N33/558 ,  G01N33/543

    Abstract: This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric ration substance having a nitrogen content of at least about two percent. In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent.

    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE
    9.
    发明专利
    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE 未知

    公开(公告)号：CA2110050A1

    公开(公告)日：1992-12-10

    申请号：CA2110050

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/547 ,  G01N33/548

    Abstract: 2110050 9221772 PCTABS00017 The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.

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