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    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS
    4.
    发明公开
    REAGENTS AND METHODS FOR PERFORMING TWO-STEP ION-CAPTURE BINDING ASSAYS 失效
    用于实施两步离子束缚测定的试剂和方法

    公开(公告)号：EP0586605A4

    公开(公告)日：1996-01-17

    申请号：EP92914861

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  FICO ROSARIO ,  JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/545 ,  G01N33/538 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/537 ,  G01N33/541 ,  G01N33/543 ,  G01N33/544 ,  G01N33/546 ,  G01N33/551

    CPC classification number: G01N33/538

    Abstract: This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent.

    DEVICES FOR PERFORMING ION-CAPTURE BINDING ASSAYS.
    6.
    发明公开
    DEVICES FOR PERFORMING ION-CAPTURE BINDING ASSAYS. 失效
    件来进行离子捕获测定。

    公开(公告)号：EP0641442A4

    公开(公告)日：1994-11-22

    申请号：EP92911929

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/545 ,  G01N33/538 ,  G01N33/543 ,  G01N33/558 ,  G01N33/574 ,  G01N33/566 ,  G01N33/544

    CPC classification number: G01N33/558 ,  G01N33/538 ,  G01N33/54366 ,  G01N33/57473

    Abstract: This invention presents novel assay devices employing a capture reagent, involving a specific binding member attached to a charged substance, and a porous material containing a capture or reaction zone that is oppositely charged with respect to the capture reagent. In one embodiment, a test sample suspected of containing the analyte of interest is contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged capture or reaction zone to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, both sandwich and competitive assays can be performed.

    9.
    发明专利
    未知

    公开(公告)号：ES2016070T3

    公开(公告)日：1997-01-16

    申请号：ES89101263

    申请日：1989-01-25

    Applicant: ABBOTT LAB

    Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

    IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/537

    Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

    10.
    发明专利
    未知

    公开(公告)号：ES2080056T3

    公开(公告)日：1996-02-01

    申请号：ES89114103

    申请日：1989-07-31

    Applicant: ABBOTT LAB

    Inventor： HILTIBRAN ROBERT G ,  STROUPE STEPHEN DENHAM ,  JOU YI-HER

    IPC: G01N33/532 ,  C07K14/435 ,  C07K14/76 ,  C08G69/10 ,  G01N33/538 ,  G01N33/542 ,  G01N33/543 ,  G01N33/574

    Abstract: This invention presents novel polymeric anionic molecules and novel negatively charged capture reagents comprising the reaction products of said anionic molecules and a specific binding member for use in separation techniques and assay procedures wherein said activated polymeric anionic molecule comprises a compound having the formula: wherein n is about 10 to about 500; z is about 1 to about 6; W is selected from the group consisting of H , Na , K , Li , amine salts, and derivatives thereof; and X is a reactive group or a structure having a reactive group that enables the chemical binding of said activated polymer to a specific binding member.

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