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公开(公告)号：WO2010039852A2
公开(公告)日：2010-04-08
申请号：PCT/US2009059059
申请日：2009-09-30
Applicant: ABBOTT LAB , HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
CPC classification number: C40B50/06 , C07K16/00 , C07K16/1018 , C07K16/241 , C07K16/244 , C07K2317/21 , C07K2317/622 , C12Q2563/185 , C40B40/08
Abstract: The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.
Abstract translation: 本发明的特征在于改进的体外RNA显示文库以允许来自表达文库的scFv抗体分子的可靠表达和选择。 本发明的scFv抗体文库含有改善表达scFv抗体表达的优化的缩短的域间连接体。 scFv抗体文库还包括允许识别单个文库克隆，文库或其子集的短核酸条形码。 还提供了用于产生，扩增和分光本发明的scFv抗体文库的引物。
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公开(公告)号：EP2340256A4
公开(公告)日：2012-03-21
申请号：EP09818451
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
CPC classification number: C40B50/06 , C07K16/00 , C07K16/1018 , C07K16/241 , C07K16/244 , C07K2317/21 , C07K2317/622 , C12Q2563/185 , C40B40/08
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公开(公告)号：EP2342562A4
公开(公告)日：2012-03-21
申请号：EP09818450
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
CPC classification number: G01N33/54326 , C12N15/1041
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公开(公告)号：MX2011003381A
公开(公告)日：2011-04-21
申请号：MX2011003381
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： CHUNG-MING-HSIEH , KUTSKOVA YULIYA A , MEMMOTT JOHN E
IPC: G01N33/53
Abstract: La presente invención, presenta métodos mejorados para despliegue de ARN in vitro, para permitir una expresión y selección confiable de moléculas de anticuerpo scFv a partir de bibliotecas de expresión. Los métodos mejorados, implican en parte, el uso de condiciones de reducción leves, las cuales favorecen el enlace de disulfuro de intra-cadena scFv y, por lo tanto, corrigen el pliegue de las moléculas de anticuerpo scFv. Aunque particularmente adecuados para expresión y selección de moléculas de anticuerpo scFv, los métodos de la presente invención, también son útiles para despliegue de ARN in vitro de todas las clases de proteína.
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公开(公告)号：CA2737035A1
公开(公告)日：2010-04-08
申请号：CA2737035
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
IPC: G01N33/53
Abstract: The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.
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Patent Agency Ranking
公开(公告)号：NZ591544A
公开(公告)日：2013-01-25
申请号：NZ59154409
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
IPC: G01N33/53
Abstract: Disclosed is a method of screening an scFv antibody RNA display library, the method comprising the steps of: (a) providing a puromycin or analogue thereof crosslinked single chain Fv (scFv) mRNA molecule, said molecule comprising an mRNA encoding a 5' scFv and a 3' spacer sequence, which molecule is crosslinked to a single stranded nucleic acid linker, the linker comprising a puromycin, or analogue thereof, at a 3' end and a Psoralen C6 at a 5' end; (b) in vitro translating the puromycin-crosslinked scFv mRNA molecule in the presence of a label under mildly reducing conditions such that a labeled puromycin-crosslinked scFv mRNA/protein molecule is formed; (c) purifying the labeled puromycin-crosslinked scFv mRNA/protein molecule; (d) subjecting the purified labeled puromycin-crosslinked scFv mRNA/protein molecule to antigen selection with at least one antigen; and (e) recovering the purified labeled puromycin-crosslinked scFv mRNA/protein molecules using affinity based magnetic beads.
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公开(公告)号：NZ591543A
公开(公告)日：2012-11-30
申请号：NZ59154309
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
Abstract: Disclosed are in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.
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公开(公告)号：HK1159250A1
公开(公告)日：2012-07-27
申请号：HK11113810
申请日：2011-12-21
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
IPC: G01N20060101 , C12N20060101
Abstract: The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.
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公开(公告)号：MX2011003380A
公开(公告)日：2011-04-21
申请号：MX2011003380
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
Abstract: La presente invención presenta genotecas de despliegue de ARN in vitro mejoradas para permitir la expresión y selección confiables de moléculas de anticuerpo de scFv a partir de las genotecas de expresión. Las genotecas de anticuerpo de scFv de la invención contienen un enlazador inter-dominio acortado, optimizado que mejora la expresión de la expresión de anticuerpo de scFv. Las genotecas de anticuerpo de scFv también incluyen códigos de barras de ácido nucleico cortos que permiten la identificación de clones de genoteca individuales, genotecas o subconjuntos de las mismas. También se proveen iniciadores para generar, amplificar y tipificar por espectros las genotecas de anticuerpo de scFv de la invención.
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公开(公告)号：CA2736430A1
公开(公告)日：2010-04-08
申请号：CA2736430
申请日：2009-09-30
Applicant: ABBOTT LAB
Inventor： HSIEH CHUNG-MING , KUTSKOVA YULIYA A , MEMMOTT JOHN E
Abstract: The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.
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