POROUS DIAPHRAGM APPARATUS FOR CHROMATO- GRAPHIC ASSAY AND METHOD OF MANUFACTURING THE SAME

    公开(公告)号:JPH03120468A

    公开(公告)日:1991-05-22

    申请号:JP26029090

    申请日:1990-09-26

    Applicant: ABBOTT LAB

    Abstract: PURPOSE: To give a mechanical strength to a nitrocellulose film while retaining hydrophilic property by laminating a wet porous film on a support with its side surface using a water-soluble solvent base adhesive. CONSTITUTION: A wet porous film 10 is laminated on a support 14 with at least one its side surface using a water-soluble solvent base adhesive 12 and is brought into contact with a biologically active reagent, thus retaining the activity of the reagent. The porous film 10 preferably consists of nitrocellulose and the adhesive 12 consists of adhesive research AS 73. Also, a surface-active agent, preferably alkyl sulfate or sulfone acid alkyl may be included. Then, the water-based solvent base adhesive 12 is used for laminating one surface or both sides of the film 10. When performing lamination only at one side of the film 10, protein is applied to a specific part 15 on the surface at the opposite side and a fluid is brought into contact from the opposite side, thus giving mechanical strength to the nitrocellulose film while retaining hydrophilic property.

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