公开(公告)号：JPS6432169A

公开(公告)日：1989-02-02

申请号：JP17365088

申请日：1988-07-12

Applicant: ABBOTT LAB

Inventor： SHIYANFUAN CHIN ,  PATORISHIA EI BIRINGU ,  JIYURIAN GOODON

IPC: G01N30/88 ,  G01N30/90 ,  G01N33/532 ,  G01N33/543 ,  G01N33/558 ,  G01N33/58 ,  G01N33/76 ,  G01N37/00

Abstract: PURPOSE: To establish a method by which a chromatographically mobile and visually detectable signals can be generated by passing a sample/indicator solution mixture containing a labeled first reagent and an object to be analyzed through a chromatographic substrate material. CONSTITUTION: A fixed amount of sample to be tested is mixed with an indicator solution composed of a first reagent labeled with colloidal particles under the presence of a chromatographic movement accelerator and the first terminal 14 of a testing device 10 is dipped in the mixture 17 contained in a container 16. The sample/indicator solution mixture containing the labeled first reagent and the object to be analyzed advances to a first zone 13 through a chromatographic substrate material 11. Then the reagent and object are competitively bonded to a second specific binding reagent immobilized to the first zone 13. However, the chromatographic solvent moves so that the object to be analyzed and the material of the first reagent labeled with colloidal particles which are not specifically bonded to the immobilized second reagent can be eliminated from the zone 13 and the movement is continued until the sample/ indicator solution mixture is exhausted.

公开(公告)号：JPH04230857A

公开(公告)日：1992-08-19

申请号：JP10416791

申请日：1991-05-09

Applicant: ABBOTT LAB

Inventor： SHIYANFUAN CHIN ,  JIYURIAN GOODAN ,  TSUNNFUI KEI JIYOU

IPC: G01N33/543 ,  G01N33/558

Abstract: PURPOSE: To provide a high sensitivity assay device capable of being used easily, accurately and quickly to detect a small amount of an analytical object. CONSTITUTION: Analyzing apparatus and method according to this invention comprises the use of a solid phase material, and a sample and bonding body move in the solid phase to sequentially make contact with at least two determined positions for relatively indicating the fixed bonding body. These two determined positions comprise a catching position for bonding a catching reagent and a bonding body recovering position for bonding a bonding body recovering reagent. The fixation of the bonding body in these positions is correlated with an amount of an analytical object in the sample.

公开(公告)号：JPH01244370A

公开(公告)日：1989-09-28

申请号：JP32320388

申请日：1988-12-21

Applicant: ABBOTT LAB

Inventor： SHIYAARON MAAFUI DEIBAROU ,  JIEIMUZU ERU UIRUKOTSUKUSU ,  TOOMASU FUREDERITSUKU HORUTSUM ,  JIYURIAN GOODON ,  SHIYANFUAN CHIN

IPC: G01N30/88 ,  G01N30/90 ,  G01N33/543 ,  G01N37/00

Abstract: PURPOSE: To form a novel assay by employing an application pad containing a diffusive indicator which can migrate from the application pad to a chromatograph substance thereby combining several elements. CONSTITUTION: The equipment for assay employs a chromatograph substance 2 which can cause migration of a liquid, and an application pad 1. The application pad 1 controls the test sample flow to chromatograph substance 2, filter the test sample and mixes an assay reagent. The assay reagent is taken into the application pad 1 and chromatograph substance 2 and produces a detectable signal when an analyte is present. Results of assay can be indicated qualitatively and quantitatively by changing the position on chromatograph substance 2 containing the reagent.