公开(公告)号：AU6710194A

公开(公告)日：1994-11-21

申请号：AU6710194

申请日：1994-04-21

Applicant: ABBOTT LAB

Inventor： COLEMAN CAROLE LYNN ,  GENGER KEVIN REINHOLD ,  GRAHAM HUGH WILLIAM ,  HANNA CHARLES FRANCIS ,  KHALIL OMAR SOLIMAN ,  WENDER DAVID C

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/77 ,  G01N33/543 ,  G01N21/03

Abstract: An improved device is used for performing solid-phase chemiluminescent immunoassays. The device comprises a container having a fibrous matrix and a porous absorbent material. An improvement in the device is the use of light absorbing material as the absorbent material or as a light barrier between the fibrous matrix and the absorbent material. A second improvement comprises a fibrous matrix of binderless fiber matrix, such as glass fiber. The invention improves the direct measurement of the chemiluminescent signal from a solid surface by reducing background signal. The device is disposable and is suitable for manual use or for use with an apparatus having programmed instructions. The device is designed to be employed in a variety of solid-phase diagnostic assays such as sandwich or competitive binding assays. The device is also designed for use with microparticle capture or ion capture methods of separating the immunochemical reaction products.

公开(公告)号：CA2159732A1

公开(公告)日：1994-11-10

申请号：CA2159732

申请日：1994-04-21

Applicant: ABBOTT LAB

Inventor： COLEMAN CAROLE LYNN ,  GENGER KEVIN REINHOLD ,  GRAHAM HUGH WILLIAM ,  HANNA CHARLES FRANCIS ,  KHALIL OMAR SOLIMAN ,  WENDER DAVID C

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/77 ,  G01N33/543 ,  G01N33/569 ,  G01N33/576 ,  G01N33/94

Abstract: An improved device is used for performing solid- phase chemiluminescent immunoassays. The device com- prises a container having a fibrous matrix and a porous ab- sorbent material. An improvement in the device is the use of light absorbing material as the absorbent material or as a light barrier between the fibrous matrix and the absorbent material. A second improvement comprises a fibrous ma- trix of binderless fiber matrix, such as glass fiber. The invention improves the direct measurement of the chemi- luminescent signal from a solid surface by reducing back- ground signal. The device is disposable and is suitable for manual use or for use with an apparatus having programmed instructions. The device is designed to be employed in a variety of solid- phase diagnostic assays such as sandwich or competitive binding assays. The devic e is also designed for use with microparticle capture or ion capture methods of separating the immunochemical reaction products.

公开(公告)号：ES2110236T3

公开(公告)日：1998-02-01

申请号：ES94914865

申请日：1994-04-21

Applicant: ABBOTT LAB

Inventor： COLEMAN CAROLE LYNN ,  GENGER KEVIN REINHOLD ,  GRAHAM HUGH WILLIAM ,  HANNA CHARLES FRANCIS ,  KHALIL OMAR SOLIMAN ,  WENDER DAVID C

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/77 ,  G01N33/543 ,  G01N21/03

Abstract: An improved device is used for performing solid-phase chemiluminescent immunoassays. The device comprises a container having a fibrous matrix and a porous absorbent material. An improvement in the device is the use of light absorbing material as the absorbent material or as a light barrier between the fibrous matrix and the absorbent material. A second improvement comprises a fibrous matrix of binderless fiber matrix, such as glass fiber. The invention improves the direct measurement of the chemiluminescent signal from a solid surface by reducing background signal. The device is disposable and is suitable for manual use or for use with an apparatus having programmed instructions. The device is designed to be employed in a variety of solid-phase diagnostic assays such as sandwich or competitive binding assays. The device is also designed for use with microparticle capture or ion capture methods of separating the immunochemical reaction products.

公开(公告)号：DE3855614T2

公开(公告)日：1997-05-15

申请号：DE3855614

申请日：1988-03-04

Applicant: ABBOTT LAB

Inventor： HOUSEMAN KENNETH RUSSELL ,  WENDER DAVID C ,  BANOVEZ LAWRENCE GEORGE ,  WROBLEWSKI MIECZYSLAW

IPC: G01N21/27 ,  G01J3/12 ,  G01N21/25 ,  G01N21/59 ,  G01N21/47 ,  G01N33/53 ,  G01N33/543

Abstract: A vertical beam spectrophotometer for measuring the light absorption of an assay prepared using standard wet chemistry procedures and conventional solid phase coated bead technology is disclosed. The spectrophotometer measures the absorption of the assay in a conventional reaction cuvette with the bead remaining in the cuvette. The light source of the spectrophotometer illuminates the bead, which diffuses the light into the surrounding assay solution. A lense projects the diffused light onto a photocell which converts it into an electrical signal having magnitude related to the light absorption of the assay. The signal is processed in a known manner by conventional processing circuitry to obtain an absorption value.

公开(公告)号：ES2095203T3

公开(公告)日：1997-02-16

申请号：ES88103398

申请日：1988-03-04

Applicant: ABBOTT LAB

Inventor： HOUSEMAN KENNETH RUSSELL ,  WENDER DAVID C ,  BANOVEZ LAWRENCE GEORGE ,  WROBLEWSKI MIECZYSLAW

IPC: G01N21/27 ,  G01J3/12 ,  G01N21/25 ,  G01N21/59 ,  G01N21/47 ,  G01N33/53 ,  G01N33/543

Abstract: A vertical beam spectrophotometer for measuring the light absorption of an assay prepared using standard wet chemistry procedures and conventional solid phase coated bead technology is disclosed. The spectrophotometer measures the absorption of the assay in a conventional reaction cuvette with the bead remaining in the cuvette. The light source of the spectrophotometer illuminates the bead, which diffuses the light into the surrounding assay solution. A lense projects the diffused light onto a photocell which converts it into an electrical signal having magnitude related to the light absorption of the assay. The signal is processed in a known manner by conventional processing circuitry to obtain an absorption value.

公开(公告)号：DE3855614D1

公开(公告)日：1996-11-21

申请号：DE3855614

申请日：1988-03-04

Applicant: ABBOTT LAB

Inventor： HOUSEMAN KENNETH RUSSELL ,  WENDER DAVID C ,  BANOVEZ LAWRENCE GEORGE ,  WROBLEWSKI MIECZYSLAW

IPC: G01N21/27 ,  G01J3/12 ,  G01N21/25 ,  G01N21/59 ,  G01N21/47 ,  G01N33/53 ,  G01N33/543

Abstract: A vertical beam spectrophotometer for measuring the light absorption of an assay prepared using standard wet chemistry procedures and conventional solid phase coated bead technology is disclosed. The spectrophotometer measures the absorption of the assay in a conventional reaction cuvette with the bead remaining in the cuvette. The light source of the spectrophotometer illuminates the bead, which diffuses the light into the surrounding assay solution. A lense projects the diffused light onto a photocell which converts it into an electrical signal having magnitude related to the light absorption of the assay. The signal is processed in a known manner by conventional processing circuitry to obtain an absorption value.

公开(公告)号：CA1316011C

公开(公告)日：1993-04-13

申请号：CA562527

申请日：1988-03-25

Applicant: ABBOTT LAB

Inventor： HOUSEMAN KENNETH R ,  WENDER DAVID C ,  BANOVEZ LAWRENCE G ,  WROBLEWSKI MIECZYSLAW

IPC: G01N21/27 ,  G01J3/12 ,  G01N21/25 ,  G01N21/59 ,  G01N21/47 ,  G01N33/53 ,  G01N33/543

Abstract: A vertical beam spectrophotometer for measuring the light absorption of an assay prepared using standard wet chemistry procedures and conventional solid phase coated bead technology is disclosed. The spectrophotometer measures the absorption of the assay in a conventional reaction cuvette with the bead remaining in the cuvette. The light source of the spectrophotometer illuminates the bead, which diffuses the light into the surrounding assay solution. A lense projects the diffused light onto a photocell which converts it into an electrical signal having magnitude related to the light absorption of the assay. The signal is processed in a known manner by conventional processing circuitry to obtain an absorption value.