中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

18 records (took 0.021 seconds)

    Method for detecting and quantifying hepatitis c virus
    1.
    发明专利
    Method for detecting and quantifying hepatitis c virus 审中-公开
    检测和定量丙型肝炎病毒的方法

    公开(公告)号：JP2010246555A

    公开(公告)日：2010-11-04

    申请号：JP2010136824

    申请日：2010-06-16

    Applicant: Abbott Lab ,  アボット・ラボラトリーズAbbott Laboratories

    Inventor： WILLIAMS GREGG T

    IPC: C12N15/09 ,  C07H21/00 ,  C12N20060101 ,  C12Q1/68 ,  C12Q1/70

    CPC classification number: C12Q1/707 ,  C12Q1/6813 ,  C12Q2545/107

    Abstract: PROBLEM TO BE SOLVED: To provide a method and a reagent for detecting and quantifying hepatitis C virus (HCV) in a biological sample, and a kit for performing the method.
    SOLUTION: In this method, the hepatitis C virus is quantified by adding a test sample suspected to contain an HCV genome nucleic acid with a known amount of endogenous control oligonucleotide containing an HCV nucleic acid sequence, a first labeled probe specific to an amplicon of the endogenous control oligonucleotide, and a second labeled probe specific to an amplicon of an HCV genome nucleic acid, competitively amplifying these substances at the same time to form a hybrid between the probe and the amplicon, and detecting the hybrid.
    COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 待解决的问题：提供生物样品中检测和定量丙型肝炎病毒（HCV）的方法和试剂，以及用于实施该方法的试剂盒。 解决方案：在该方法中，通过将怀疑含有HCV基因组核酸的测试样品与已知量的含有HCV核酸序列的内源性对照寡核苷酸，第一标记的探针 内源性对照寡核苷酸的扩增子和对HCV基因组核酸的扩增子特异的第二标记探针，同时竞争性地扩增这些物质，以形成探针和扩增子之间的杂交体，并检测杂交体。 版权所有（C）2011，JPO＆INPIT

    DETERMINATION OF STEROIDS BY COMPETITIVE IMMUNOASSAY
    3.
    发明公开
    DETERMINATION OF STEROIDS BY COMPETITIVE IMMUNOASSAY 失效
    类固醇的竞争性免疫测定

    公开(公告)号：EP0813684A1

    公开(公告)日：1997-12-29

    申请号：EP96908511

    申请日：1996-02-26

    Applicant: ABBOTT LAB

    Inventor： WILLIAMS GREGG T ,  GROSKOPF WILLIAM R ,  BAKER HAROLD N ,  AGDEPPA DALMACIO A

    IPC: G01N33/53 ,  C07J41/00 ,  G01N33/535 ,  G01N33/543 ,  G01N33/74

    CPC classification number: G01N33/743 ,  C07J41/0016 ,  C07J41/0038 ,  G01N33/535 ,  Y10S435/806 ,  Y10S436/906

    Abstract: A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of: a) incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase; b) separating said solid phase from said mixture; c) measuring the amount of label present in said mixture or in said solid phase; and d) determining the amount of steroid in said sample from the amount of label. The kit comprises a solid phase coupled to an antibody specific for a steroid and a conjugate of an analogue of that steroid.

    5.
    发明专利
    未知

    公开(公告)号：ES2176445T3

    公开(公告)日：2002-12-01

    申请号：ES96908511

    申请日：1996-02-26

    Applicant: ABBOTT LAB

    Inventor： WILLIAMS GREGG T ,  GROSKOPF WILLIAM R ,  BAKER HAROLD N ,  AGDEPPA DALMACIO A

    IPC: G01N33/53 ,  C07J41/00 ,  G01N33/535 ,  G01N33/543 ,  G01N33/74

    Abstract: A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone.The method comprises the steps of:a. incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase;b. separating said solid phase from said mixture;c. measuring the amount of label present in said mixture or in said solid phase; andd. determining the amount of steroid in said sample from the amount of label.The kit comprises a solid phase coupled to an antibody specific for a steroid and a conjugate of an analogue of that steroid.

    6.
    发明专利
    未知

    公开(公告)号：ES2184812T3

    公开(公告)日：2003-04-16

    申请号：ES95939157

    申请日：1995-11-13

    Applicant: ABBOTT LAB

    Inventor： WILLIAMS GREGG T ,  KUEMMERLE STEVEN C ,  ABOLENEEN HODA I ,  GROSKOPF WILLIAM R

    IPC: A61K47/18 ,  G01N33/531 ,  G01N33/74 ,  G01N33/53 ,  G01N33/96 ,  A61K31/56

    Abstract: Aqueous solutions of steroid compounds which have biological activity and have a tendency to oxidative degradation at temperatures between 2 and 8° C. on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

Patent Agency Ranking