公开(公告)号：CA2204027A1

公开(公告)日：1996-06-06

申请号：CA2204027

申请日：1995-11-13

Applicant: ABBOTT LAB

Inventor： ABOLENEEN HODA I ,  GROSKOPF WILLIAM R ,  KUEMMERLE STEVEN C ,  WILLIAMS GREGG T

IPC: G01N33/53 ,  A61K47/18 ,  G01N33/531 ,  G01N33/74 ,  G01N33/96

Abstract: Aqueous solutions of steroid compounds which have biological activity and have a tendency to oxidative degradation at temperatures between 2 and 8 ~C on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

公开(公告)号：DE69121635T2

公开(公告)日：1997-04-03

申请号：DE69121635

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：ES2184812T3

公开(公告)日：2003-04-16

申请号：ES95939157

申请日：1995-11-13

Applicant: ABBOTT LAB

Inventor： WILLIAMS GREGG T ,  KUEMMERLE STEVEN C ,  ABOLENEEN HODA I ,  GROSKOPF WILLIAM R

IPC: A61K47/18 ,  G01N33/531 ,  G01N33/74 ,  G01N33/53 ,  G01N33/96 ,  A61K31/56

Abstract: Aqueous solutions of steroid compounds which have biological activity and have a tendency to oxidative degradation at temperatures between 2 and 8° C. on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

公开(公告)号：DE69121635D1

公开(公告)日：1996-10-02

申请号：DE69121635

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：CA2043862A1

公开(公告)日：1991-12-07

申请号：CA2043862

申请日：1991-06-04

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN M ,  MATTINGLY PHILLIP G ,  WELCH CHRISTOPHER J ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/544 ,  G01N33/535

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：CA2204027C

公开(公告)日：2006-03-14

申请号：CA2204027

申请日：1995-11-13

Applicant: ABBOTT LAB

Inventor： ABOLENEEN HODA I ,  KUEMMERLE STEVEN C ,  WILLIAMS GREGG T ,  GROSKOPF WILLIAM R

IPC: G01N33/53 ,  G01N33/74 ,  A61K47/18 ,  G01N33/531 ,  G01N33/96

Abstract: Aqueous solutions of steroid compounds which have biological activity and ha ve a tendency to oxidative degradation at temperatures between 2 and 8 .degree.C on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

公开(公告)号：AT223060T

公开(公告)日：2002-09-15

申请号：AT95939157

申请日：1995-11-13

Applicant: ABBOTT LAB

Inventor： WILLIAMS GREGG T ,  KUEMMERLE STEVEN C ,  ABOLENEEN HODA I ,  GROSKOPF WILLIAM R

IPC: G01N33/53 ,  A61K47/18 ,  G01N33/531 ,  G01N33/74 ,  G01N33/96 ,  A61K31/56

Abstract: Aqueous solutions of steroid compounds which have biological activity and have a tendency to oxidative degradation at temperatures between 2 and 8° C. on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

公开(公告)号：ES2093655T3

公开(公告)日：1997-01-01

申请号：ES91109036

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.