公开(公告)号：WO2008147982A1

公开(公告)日：2008-12-04

申请号：PCT/US2008/064688

申请日：2008-05-23

Applicant: ABBOTT LABORATORIES ,  BRATE, Elaine, M. ,  FINLEY, David, M. ,  HOLETS-MCCORMACK, Shelley, R. ,  PACENTI, David, P. ,  PIKTEL, Ryan, E. ,  SHIELDS, Michelle ,  SPRING, Thomas, G. ,  WANG, Philip, P.

Inventor： BRATE, Elaine, M. ,  FINLEY, David, M. ,  HOLETS-MCCORMACK, Shelley, R. ,  PACENTI, David, P. ,  PIKTEL, Ryan, E. ,  SHIELDS, Michelle ,  SPRING, Thomas, G. ,  WANG, Philip, P.

IPC: G01N33/543 ,  G01N33/94

CPC classification number: G01N33/54393 ,  G01N33/9493

Abstract: The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

Abstract translation: 本公开提供了与分析物代谢物显示降低的交叉反应性的免疫测定法。 此外，本公开提供诊断免疫测定以确定在体内或体外代谢形成交叉反应代谢物的疏水性药物的测试样品中的浓度或水平，其中与药物分析物的这种代谢物的交叉反应性降低。 具体地，本公开提供了这样的免疫测定，其中疏水性药物是免疫抑制剂药物，例如环孢菌素A.

公开(公告)号：WO1995017679A1

公开(公告)日：1995-06-29

申请号：PCT/US1994014716

申请日：1994-12-19

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: G01N33/84

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Abstract translation: 检测样品中存在的铅，包括以下步骤：（a）将铅回收剂加入到含有铅的测定溶液中; （b）向测定溶液中加入在铅存在下被抑制的二硫化物酶; 和（c）将二硫键的活性与样品中的铅的量相关联。 铅回收剂，优选铅螯合剂，提高了测定的灵敏度和准确性，使得该测定可以容易地自动化，以便使用市售的自动化系统检测全血中的铅。

公开(公告)号：EP2174137B1

公开(公告)日：2011-11-30

申请号：EP08769692.8

申请日：2008-05-23

Applicant: Abbott Laboratories

Inventor： BRATE, Elaine, M. ,  FINLEY, David, M. ,  HOLETS-MCCORMACK, Shelley, R. ,  PACENTI, David, P. ,  PIKTEL, Ryan, E. ,  SHIELDS, Michelle ,  SPRING, Thomas, G. ,  WANG, Philip, P.

IPC: G01N33/543 ,  G01N33/94

CPC classification number: G01N33/54393 ,  G01N33/9493

Abstract: The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

公开(公告)号：EP0736181A1

公开(公告)日：1996-10-09

申请号：EP95906047.0

申请日：1994-12-19

Applicant: Abbott Laboratories

Inventor： WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: C12Q1 ,  G01N21 ,  G01N31 ,  G01N33

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

公开(公告)号：EP2174137A1

公开(公告)日：2010-04-14

申请号：EP08769692.8

申请日：2008-05-23

Applicant: Abbott Laboratories

Inventor： BRATE, Elaine, M. ,  FINLEY, David, M. ,  HOLETS-MCCORMACK, Shelley, R. ,  PACENTI, David, P. ,  PIKTEL, Ryan, E. ,  SHIELDS, Michelle ,  SPRING, Thomas, G. ,  WANG, Philip, P.

IPC: G01N33/543 ,  G01N33/94

CPC classification number: G01N33/54393 ,  G01N33/9493

Abstract: The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

公开(公告)号：EP1320753A2

公开(公告)日：2003-06-25

申请号：EP01971231.4

申请日：2001-09-20

Applicant: Abbott Laboratories

Inventor： SCOPP, Richard, L. ,  FINLEY, David, M. ,  TRIMPE, Kevin, L. ,  LACH, Agnieszka ,  PESTEL, Cynthia, D. ,  RAMP, John, M.

IPC: G01N33/53

CPC classification number: G01N33/57434 ,  G01N33/5306 ,  G01N33/54333 ,  G01N33/54393 ,  G01N33/76

Abstract: Methods and kits are provided for decreasing interferences and inaccuracies due to nonoptimal sample handling of blood samples in plasma or serum containing assay samples of specific binding assays by addition of a large polycation to the assay sample during the specific binding assay.