公开(公告)号：WO1997024621A2

公开(公告)日：1997-07-10

申请号：PCT/US1996019544

申请日：1996-12-16

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  ADAMCZYK, Maciej ,  FISHPAUGH, Jeffrey, R. ,  HEUSER, Kevin ,  RAMP, John, M. ,  WONG, Martin

IPC: G01N33/72

CPC classification number: C07D207/337 ,  C07D405/12 ,  G01N33/532 ,  G01N33/72 ,  G01N33/84

Abstract: Immunoassay methods and reagents for measuring the levels of lead in a test sample via the quantification of porphobilinogen use antibodies prepared with compounds of Formulas (II) and (III), wherein X and Y are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to an immunogenic carrier material P wherein P is preferably bovine serum albumin. Also described are the synthesis of fluorescein tracers of the structure of Formulas (V) and (VII), wherein A and C are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to a detectable moiety Q, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.

Abstract translation: 用于通过定量用式（II）和（III）化合物制备的胆色素原使用抗体来测定测试样品中铅含量的免疫测定方法和试剂，其中X和Y是由0至2个杂原子组成的连接基团和0至 8个碳原子与免疫原性载体材料P连接，其中P优选为牛血清白蛋白。 还描述了式（V）和（VII）结构的荧光素示踪剂的合成，其中A和C是连接到可检测部分Q的0至2个杂原子和0至8个碳原子组成的连接基团，其中Q 是可检测的部分，优选荧光素或荧光素衍生物。

公开(公告)号：WO1995017679A1

公开(公告)日：1995-06-29

申请号：PCT/US1994014716

申请日：1994-12-19

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: G01N33/84

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Abstract translation: 检测样品中存在的铅，包括以下步骤：（a）将铅回收剂加入到含有铅的测定溶液中; （b）向测定溶液中加入在铅存在下被抑制的二硫化物酶; 和（c）将二硫键的活性与样品中的铅的量相关联。 铅回收剂，优选铅螯合剂，提高了测定的灵敏度和准确性，使得该测定可以容易地自动化，以便使用市售的自动化系统检测全血中的铅。

公开(公告)号：EP0736181A1

公开(公告)日：1996-10-09

申请号：EP95906047.0

申请日：1994-12-19

Applicant: Abbott Laboratories

Inventor： WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: C12Q1 ,  G01N21 ,  G01N31 ,  G01N33

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

公开(公告)号：EP0876616A2

公开(公告)日：1998-11-11

申请号：EP96943648.0

申请日：1996-12-16

Applicant: Abbott Laboratories

Inventor： ADAMCZYK, Maciej ,  FISHPAUGH, Jeffrey, R. ,  HEUSER, Kevin ,  RAMP, John, M. ,  WONG, Martin

IPC: C07D207 ,  C07D405 ,  C07K16 ,  G01N33

CPC classification number: C07D207/337 ,  C07D405/12 ,  G01N33/532 ,  G01N33/72 ,  G01N33/84

Abstract: Immunoassay methods and reagents for measuring the levels of lead in a test sample via the quantification of porphobilinogen use antibodies prepared with compounds of Formulas (II) and (III), wherein X and Y are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to an immunogenic carrier material P wherein P is preferably bovine serum albumin. Also described are the synthesis of fluorescein tracers of the structure of Formulas (V) and (VII), wherein A and C are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to a detectable moiety Q, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.