公开(公告)号：WO1995017679A1

公开(公告)日：1995-06-29

申请号：PCT/US1994014716

申请日：1994-12-19

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: G01N33/84

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Abstract translation: 检测样品中存在的铅，包括以下步骤：（a）将铅回收剂加入到含有铅的测定溶液中; （b）向测定溶液中加入在铅存在下被抑制的二硫化物酶; 和（c）将二硫键的活性与样品中的铅的量相关联。 铅回收剂，优选铅螯合剂，提高了测定的灵敏度和准确性，使得该测定可以容易地自动化，以便使用市售的自动化系统检测全血中的铅。

公开(公告)号：EP0736181A1

公开(公告)日：1996-10-09

申请号：EP95906047.0

申请日：1994-12-19

Applicant: Abbott Laboratories

Inventor： WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: C12Q1 ,  G01N21 ,  G01N31 ,  G01N33

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.